Literature DB >> 8192681

Purification and properties of porphobilinogen deaminase from Arabidopsis thaliana.

R M Jones1, P M Jordan.   

Abstract

Porphobilinogen deaminase (EC 4.3.1.8) has been purified to homogeneity (16,000-fold) from the plant Arabidopsis thaliana in yields of 8%. The deaminase is a monomer of M(r) 35,000, as shown by SDS/PAGE, and 31,000, using gel-filtration chromatography. The pure enzyme has a Vmax. of 4.5 mumol/h per mg and a Km of 17 +/- 4 microM. Determination of the pI and pH optimum revealed values of 5.2 and 8.0 respectively. The sequence of the N-terminus was found to be NH2-XVAVEQKTRTAI. The deaminase is heat-stable up to 70 degrees C and is inhibited by NH3 and hydroxylamine. The enzyme is inactivated by arginine-, histidine- and lysine-specific reagents. Incubation with the substrate analogue and suicide inhibitor, 2-bromoporphobilinogen, results in chain termination and in inactivation.

Entities:  

Mesh：

Substances：

Year:  1994        PMID： 8192681      PMCID： PMC1138105          DOI： 10.1042/bj2990895

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  42 in total

1.  The occurrence and determination of delta-amino-levulinic acid and porphobilinogen in urine.

Authors:  D MAUZERALL; S GRANICK
Journal:  J Biol Chem       Date:  1956-03       Impact factor: 5.157

2.  Structure of porphobilinogen deaminase reveals a flexible multidomain polymerase with a single catalytic site.

Authors:  G V Louie; P D Brownlie; R Lambert; J B Cooper; T L Blundell; S P Wood; M J Warren; S C Woodcock; P M Jordan
Journal:  Nature       Date:  1992-09-03       Impact factor: 49.962

3.  The spectrophotometric determination of uroporphyrin.

Authors:  C RIMINGTON; S L SVEINSSON
Journal:  Scand J Clin Lab Invest       Date:  1950       Impact factor: 1.713

4.  Purification and properties of uroporphyrinogen I synthetase from Rhodopseudomonas spheroides.

Authors:  P M Jordan; D Shemin
Journal:  J Biol Chem       Date:  1973-02-10       Impact factor: 5.157

5.  A dipyrrylmethane intermediate in the enzymatic synthesis of uroporphyrinogen.

Authors:  J Pluscec; L Bogorad
Journal:  Biochemistry       Date:  1970-11-24       Impact factor: 3.162

6.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

7.  Evidence for participation of aspartate-84 as a catalytic group at the active site of porphobilinogen deaminase obtained by site-directed mutagenesis of the hemC gene from Escherichia coli.

Authors:  S C Woodcock; P M Jordan
Journal:  Biochemistry       Date:  1994-03-08       Impact factor: 3.162

8.  Structure and expression of chloroplast-localized porphobilinogen deaminase from pea (Pisum sativum L.) isolated by redundant polymerase chain reaction.

Authors:  M Witty; A D Wallace-Cook; H Albrecht; A J Spano; H Michel; J Shabanowitz; D F Hunt; M P Timko; A G Smith
Journal:  Plant Physiol       Date:  1993-09       Impact factor: 8.340

9.  Studies on porphobilinogen-deaminase from Saccharomyces cerevisiae.

Authors:  S R Correa García; M V Rossetti; A M Batlle
Journal:  Z Naturforsch C J Biosci       Date:  1991 Nov-Dec

10.  Polypyrroles formed from porphobilinogen and amines by uroporphyrinogen synthetase of Rhodopseudomonas spheroides.

Authors:  R C Davies; A Neuberger
Journal:  Biochem J       Date:  1973-07       Impact factor: 3.857
View more
  8 in total

Review 1.  Porphobilinogen deaminase and uroporphyrinogen III synthase: structure, molecular biology, and mechanism.

Authors:  P M Shoolingin-Jordan
Journal:  J Bioenerg Biomembr       Date:  1995-04       Impact factor: 2.945

2.  Subcellular location of the tetrapyrrole synthesis enzyme porphobilinogen deaminase in higher plants: an immunological investigation.

Authors:  M Witty; R M Jones; M S Robb; P M Jordan; A G Smith
Journal:  Planta       Date:  1996       Impact factor: 4.116

3.  Porphobilinogen deaminase is encoded by a single gene in Arabidopsis thaliana and is targeted to the chloroplasts.

Authors:  S H Lim; M Witty; A D Wallace-Cook; L I Ilag; A G Smith
Journal:  Plant Mol Biol       Date:  1994-11       Impact factor: 4.076

4.  The common origins of the pigments of life-early steps of chlorophyll biosynthesis.

Authors:  Y J Avissar; P A Moberg
Journal:  Photosynth Res       Date:  1995-06       Impact factor: 3.573

5.  Crystallization and preliminary X-ray characterization of the tetrapyrrole-biosynthetic enzyme porphobilinogen deaminase from Arabidopsis thaliana.

Authors:  A Roberts; R Gill; R J Hussey; H Mikolajek; P T Erskine; J B Cooper; S P Wood; E J T Chrystal; P M Shoolingin-Jordan
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-11-14

6.  A pepper MSRB2 gene confers drought tolerance in rice through the protection of chloroplast-targeted genes.

Authors:  Joung Sug Kim; Hyang-Mi Park; Songhwa Chae; Tae-Ho Lee; Duk-Ju Hwang; Sung-Dug Oh; Jong-Sug Park; Dae-Geun Song; Cheol-Ho Pan; Doil Choi; Yul-Ho Kim; Baek Hie Nahm; Yeon-Ki Kim
Journal:  PLoS One       Date:  2014-03-10       Impact factor: 3.240

Review 7.  A Recap of Heme Metabolism towards Understanding Protoporphyrin IX Selectivity in Cancer Cells.

Authors:  Martin Kiening; Norbert Lange
Journal:  Int J Mol Sci       Date:  2022-07-19       Impact factor: 6.208

8.  PORPHOBILINOGEN DEAMINASE deficiency alters vegetative and reproductive development and causes lesions in Arabidopsis.

Authors:  Víctor Quesada; Raquel Sarmiento-Mañús; Rebeca González-Bayón; Andrea Hricová; María Rosa Ponce; José Luis Micol
Journal:  PLoS One       Date:  2013-01-08       Impact factor: 3.240
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.