Literature DB >> 3469667

Transformation of normal rat kidney (NRK) cells by an infectious retrovirus carrying a synthetic rat type alpha transforming growth factor gene.

S Watanabe, E Lazar, M B Sporn.   

Abstract

We synthesized a gene for rat type alpha transforming growth factor (TGF-alpha) consisting of the leader sequence and the sequence coding for the mature 50-amino acid peptide without the C-terminal processed region. This gene was inserted into the retrovirus vector pSW272, which is derived from spleen necrosis virus, to obtain an infectious recombinant virus carrying the rat TGF-alpha gene. This recombinant virus can infect normal rat kidney (NRK) cells and allow these cells to grow in soft agar in the presence of TGF-beta. Transformed cells isolated from colonies grown in soft agar contain an integrated form of the recombinant virus and secrete biologically active TGF-alpha into their medium. These results show that a biologically active TGF-alpha can be produced by a gene synthesized from only part of the coding region and that the infectious retrovirus carrying the TGF-alpha gene (SW355) can function as a transforming agent in NRK cells in the presence of TGF-beta.

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Year:  1987        PMID： 3469667      PMCID： PMC304406          DOI： 10.1073/pnas.84.5.1258

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  36 in total

1.  The murine transforming growth factor-beta precursor.

Authors:  R Derynck; J A Jarrett; E Y Chen; D V Goeddel
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2.  Control of multiplication of uninfected rat cells and rat cells converted by murine sarcoma virus.

Authors:  H M Temin
Journal:  J Cell Physiol       Date:  1970-02       Impact factor: 6.384

3.  "Contact inhibition" of cell division in 3T3 cells.

Authors:  R W Holley; J A Kiernan
Journal:  Proc Natl Acad Sci U S A       Date:  1968-05       Impact factor: 11.205

Review 4.  Transforming growth factors.

Authors:  A B Roberts; M B Sporn
Journal:  Cancer Surv       Date:  1985

5.  Rat epidermal growth factor: complete amino acid sequence. Homology with the corresponding murine and human proteins; isolation of a form truncated at both ends with full in vitro biological activity.

Authors:  R J Simpson; J A Smith; R L Moritz; M J O'Hare; P S Rudland; J R Morrison; C J Lloyd; B Grego; A W Burgess; E C Nice
Journal:  Eur J Biochem       Date:  1985-12-16

6.  Human transforming growth factor-beta complementary DNA sequence and expression in normal and transformed cells.

Authors:  R Derynck; J A Jarrett; E Y Chen; D H Eaton; J R Bell; R K Assoian; A B Roberts; M B Sporn; D V Goeddel
Journal:  Nature       Date:  1985 Aug 22-28       Impact factor: 49.962

7.  Expression of a hemopoietic growth factor cDNA in a factor-dependent cell line results in autonomous growth and tumorigenicity.

Authors:  R A Lang; D Metcalf; N M Gough; A R Dunn; T J Gonda
Journal:  Cell       Date:  1985-12       Impact factor: 41.582

8.  Developmental expression of rat transforming growth factor-alpha mRNA.

Authors:  D C Lee; R Rochford; G J Todaro; L P Villarreal
Journal:  Mol Cell Biol       Date:  1985-12       Impact factor: 4.272

9.  Total synthesis of a gene for bovine rhodopsin.

Authors:  L Ferretti; S S Karnik; H G Khorana; M Nassal; D D Oprian
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

10.  Expression in rat fibroblasts of a human transforming growth factor-alpha cDNA results in transformation.

Authors:  A Rosenthal; P B Lindquist; T S Bringman; D V Goeddel; R Derynck
Journal:  Cell       Date:  1986-07-18       Impact factor: 41.582
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  26 in total

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Authors:  Jaime Acquaviva; Hyun Jung Jun; Julie Lessard; Rolando Ruiz; Haihao Zhu; Melissa Donovan; Steve Woolfenden; Abraham Boskovitz; Ami Raval; Roderick T Bronson; Rolf Pfannl; Charles A Whittaker; David E Housman; Al Charest
Journal:  Cancer Res       Date:  2011-10-10       Impact factor: 12.701

2.  Transforming growth factor alpha: an aromatic side chain at position 38 is essential for biological activity.

Authors:  E Lazar; E Vicenzi; E Van Obberghen-Schilling; B Wolff; S Dalton; S Watanabe; M B Sporn
Journal:  Mol Cell Biol       Date:  1989-02       Impact factor: 4.272

3.  Expression of recombinant platelet-derived growth factor A- and B-chain homodimers in rat-1 cells and human fibroblasts reveals differences in protein processing and autocrine effects.

Authors:  M Bywater; F Rorsman; E Bongcam-Rudloff; G Mark; A Hammacher; C H Heldin; B Westermark; C Betsholtz
Journal:  Mol Cell Biol       Date:  1988-07       Impact factor: 4.272

4.  Possible involvement of normal p21 H-ras in the insulin/insulinlike growth factor 1 signal transduction pathway.

Authors:  B M Burgering; A J Snijders; J A Maassen; A J van der Eb; J L Bos
Journal:  Mol Cell Biol       Date:  1989-10       Impact factor: 4.272

5.  Transforming growth factor-alpha: characterization of the BamHI, RsaI, and TaqI polymorphic regions.

Authors:  J F Qian; J Feingold; C Stoll; E May
Journal:  Am J Hum Genet       Date:  1993-07       Impact factor: 11.025

6.  Hypertrophic gastropathy resembling Ménétrier's disease in transgenic mice overexpressing transforming growth factor alpha in the stomach.

Authors:  H Takagi; C Jhappan; R Sharp; G Merlino
Journal:  J Clin Invest       Date:  1992-09       Impact factor: 14.808

7.  Expression of mRNA for epidermal growth factor, transforming growth factor-alpha and their receptor in human prostate tissue and cell lines.

Authors:  K Z Ching; E Ramsey; N Pettigrew; R D'Cunha; M Jason; J G Dodd
Journal:  Mol Cell Biochem       Date:  1993-09-22       Impact factor: 3.396

8.  Overexpression of the epidermal growth factor receptor in human pancreatic cancer is associated with concomitant increases in the levels of epidermal growth factor and transforming growth factor alpha.

Authors:  M Korc; B Chandrasekar; Y Yamanaka; H Friess; M Buchier; H G Beger
Journal:  J Clin Invest       Date:  1992-10       Impact factor: 14.808

9.  Anti-sense transforming growth factor alpha oligonucleotides inhibit autocrine stimulated proliferation of a colon carcinoma cell line.

Authors:  A M Sizeland; A W Burgess
Journal:  Mol Biol Cell       Date:  1992-11       Impact factor: 4.138

10.  Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.

Authors:  E Lazar; S Watanabe; S Dalton; M B Sporn
Journal:  Mol Cell Biol       Date:  1988-03       Impact factor: 4.272
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