Literature DB >> 3459590

Expression in rat fibroblasts of a human transforming growth factor-alpha cDNA results in transformation.

A Rosenthal, P B Lindquist, T S Bringman, D V Goeddel, R Derynck.   

Abstract

Acquisition of the ability to produce and respond to a growth factor may result in increased cellular proliferation and could lead to malignant transformation. The fact that a large variety of tumor cells secrete transforming growth factor-alpha (TGF-alpha) suggests involvement of TGF-alpha in cellular transformation and provides supporting evidence for the autocrine stimulation model. In order to determine directly the role of TGF-alpha in tumorigenicity, we introduced a human TGF-alpha cDNA expression vector into established nontransformed Fischer rat fibroblast (Rat-1) cells. Synthesis and secretion of human TGF-alpha by these cells results in the loss of anchorage-dependent growth and induces tumor formation in nude mice. Anti-human TGF-alpha monoclonal antibodies prevent TGF-alpha expressing Rat-1 cells from forming colonies in soft agar.

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Year:  1986        PMID： 3459590     DOI： 10.1016/0092-8674(86)90747-6

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  81 in total

1.  Autocrine ligand binding to cell receptors. Mathematical analysis of competition by solution "decoys".

Authors:  K E Forsten; D A Lauffenburger
Journal:  Biophys J       Date:  1992-02       Impact factor: 4.033

2.  Heparin-binding epidermal growth factor-like growth factor, a v-Jun target gene, induces oncogenic transformation.

Authors:  S l Fu; I Bottoli; M Goller; P K Vogt
Journal:  Proc Natl Acad Sci U S A       Date:  1999-05-11       Impact factor: 11.205

3.  Structure-function analysis of human transforming growth factor-alpha by site-directed mutagenesis.

Authors:  J A Feild; R H Reid; D J Rieman; T P Kline; G Sathe; R G Greig; M A Anzano
Journal:  Biochem J       Date:  1992-04-01       Impact factor: 3.857

4.  Analysis of the fibroblast transformation potential of GTPase-deficient gip2 oncogenes.

Authors:  S K Gupta; C Gallego; J M Lowndes; C M Pleiman; C Sable; B J Eisfelder; G L Johnson
Journal:  Mol Cell Biol       Date:  1992-01       Impact factor: 4.272

5.  Immunocytochemical expression of growth factors by odontogenic jaw cysts.

Authors:  T Li; R M Browne; J B Matthews
Journal:  Mol Pathol       Date:  1997-02

6.  Epidermal growth factor receptor cytoplasmic domain mutations trigger ligand-independent transformation.

Authors:  S Massoglia; A Gray; T J Dull; S Munemitsu; H J Kun; J Schlessinger; A Ullrich
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

7.  Epidermal growth factor and transforming growth factor-alpha: differential intracellular routing and processing of ligand-receptor complexes.

Authors:  R Ebner; R Derynck
Journal:  Cell Regul       Date:  1991-08

8.  Possible involvement of normal p21 H-ras in the insulin/insulinlike growth factor 1 signal transduction pathway.

Authors:  B M Burgering; A J Snijders; J A Maassen; A J van der Eb; J L Bos
Journal:  Mol Cell Biol       Date:  1989-10       Impact factor: 4.272

9.  Interleukin 1 alpha and tumor necrosis factor alpha stimulate autocrine amphiregulin expression and proliferation of human papillomavirus-immortalized and carcinoma-derived cervical epithelial cells.

Authors:  C D Woodworth; E McMullin; M Iglesias; G D Plowman
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-28       Impact factor: 11.205

10.  Transforming growth factor-alpha: characterization of the BamHI, RsaI, and TaqI polymorphic regions.

Authors:  J F Qian; J Feingold; C Stoll; E May
Journal:  Am J Hum Genet       Date:  1993-07       Impact factor: 11.025
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