Literature DB >> 2710128

Transforming growth factor alpha: an aromatic side chain at position 38 is essential for biological activity.

E Lazar1, E Vicenzi, E Van Obberghen-Schilling, B Wolff, S Dalton, S Watanabe, M B Sporn.   

Abstract

Site-directed mutagenesis has been performed in the human transforming growth factor alpha gene. When tyrosine 38 is mutated into phenylalanine or tryptophane, biological activity is retained. In contrast, other alterations between cysteine 34 and cysteine 43 and disruption of disulfide bonds 8 to 21 and 34 to 43 resulted in loss of activities. The presence of an aromatic side chain at position 38 of transforming growth factor alpha seems to be essential for its activity.

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Year:  1989        PMID： 2710128      PMCID： PMC362669          DOI： 10.1128/mcb.9.2.860-864.1989

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  33 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Growth factors from murine sarcoma virus-transformed cells.

Authors:  J E de Larco; G J Todaro
Journal:  Proc Natl Acad Sci U S A       Date:  1978-08       Impact factor: 11.205

3.  Epidermal growth factor. Location of disulfide bonds.

Authors:  C R Savage; J H Hash; S Cohen
Journal:  J Biol Chem       Date:  1973-11-25       Impact factor: 5.157

4.  Proteinase mutants of Saccharomyces cerevisiae.

Authors:  E W Jones
Journal:  Genetics       Date:  1977-01       Impact factor: 4.562

5.  Rat transforming growth factor type 1: structure and relation to epidermal growth factor.

Authors:  H Marquardt; M W Hunkapiller; L E Hood; G J Todaro
Journal:  Science       Date:  1984-03-09       Impact factor: 47.728

6.  Human transforming growth factor-alpha: precursor structure and expression in E. coli.

Authors:  R Derynck; A B Roberts; M E Winkler; E Y Chen; D V Goeddel
Journal:  Cell       Date:  1984-08       Impact factor: 41.582

7.  Sarcoma growth factor from conditioned medium of virally transformed cells is composed of both type alpha and type beta transforming growth factors.

Authors:  M A Anzano; A B Roberts; J M Smith; M B Sporn; J E De Larco
Journal:  Proc Natl Acad Sci U S A       Date:  1983-10       Impact factor: 11.205

8.  Transforming growth factor alpha: mutation of aspartic acid 47 and leucine 48 results in different biological activities.

Authors:  E Lazar; S Watanabe; S Dalton; M B Sporn
Journal:  Mol Cell Biol       Date:  1988-03       Impact factor: 4.272

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Epidermal growth factor-like transforming growth factor. II. Interaction with epidermal growth factor receptors in human placenta membranes and A431 cells.

Authors:  J Massagué
Journal:  J Biol Chem       Date:  1983-11-25       Impact factor: 5.157
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  6 in total

1.  Substitution of lysine for arginine at position 42 of human transforming growth factor-alpha eliminates biological activity without changing internal disulfide bonds.

Authors:  D Defeo-Jones; J Y Tai; G A Vuocolo; R J Wegrzyn; T L Schofield; M W Riemen; A Oliff
Journal:  Mol Cell Biol       Date:  1989-09       Impact factor: 4.272

2.  Aromaticity at position 37 in human epidermal growth factor is not obligatory for activity.

Authors:  D A Engler; M R Hauser; J S Cook; S K Niyogi
Journal:  Mol Cell Biol       Date:  1991-05       Impact factor: 4.272

3.  The amphiregulin gene encodes a novel epidermal growth factor-related protein with tumor-inhibitory activity.

Authors:  G D Plowman; J M Green; V L McDonald; M G Neubauer; C M Disteche; G J Todaro; M Shoyab
Journal:  Mol Cell Biol       Date:  1990-05       Impact factor: 4.272

4.  Comparison of the antisecretory and antiulcer activity of epidermal growth factor, urogastrone and transforming growth factor alpha and its derivative in rodents in vivo.

Authors:  S M A Bastaki; S I Chandranath; J Singh
Journal:  Mol Cell Biochem       Date:  2002-07       Impact factor: 3.396

5.  NeissLock provides an inducible protein anhydride for covalent targeting of endogenous proteins.

Authors:  Arne H A Scheu; Sheryl Y T Lim; Felix J Metzner; Shabaz Mohammed; Mark Howarth
Journal:  Nat Commun       Date:  2021-01-29       Impact factor: 14.919

6.  Epidermal growth factor receptor binding is affected by structural determinants in the toxin domain of transforming growth factor-alpha-Pseudomonas exotoxin fusion proteins.

Authors:  G M Edwards; D DeFeo-Jones; J Y Tai; G A Vuocolo; D R Patrick; D C Heimbrook; A Oliff
Journal:  Mol Cell Biol       Date:  1989-07       Impact factor: 5.069
  6 in total

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