Literature DB >> 3415222

A method to assess the bacterial content of refrigerated meat.

B Perez de Castro1, M A Asensio, B Sanz, J A Ordoñez.   

Abstract

A new method has been developed to estimate the levels of gram-negative bacteria on refrigerated meat. The method is based on the aminopeptidase activity of these bacteria, which cleaves L-alanine-p-nitroanilide to yield p-nitroaniline, which is easily determined spectrophotometrically. This method allows the determination of levels around 10(6) to 10(7) CFU cm-2 in about 3 h. Because of the yellow color of p-nitroaniline, bacterial loads around 10(7) CFU cm-2 develop a color intense enough to be detected with the naked eye.

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Year:  1988        PMID： 3415222      PMCID： PMC202680          DOI： 10.1128/aem.54.6.1462-1465.1988

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  9 in total

1.  The development of aerobic spoilage flora on meat stored at chill temperatures.

Authors:  C O Gill; K G Newton
Journal:  J Appl Bacteriol       Date:  1977-10

2.  Comparison of homogenizing, shaking, and blending on the recovery of microorganisms and endotoxins from fresh and frozen ground beef as assessed by plate counts and the Limulus amoebocyte lysate test.

Authors:  J M Jay; S Margitic
Journal:  Appl Environ Microbiol       Date:  1979-11       Impact factor: 4.792

3.  A new method for evaluating surface contamination of raw meat.

Authors:  M L Williams
Journal:  J Appl Bacteriol       Date:  1967-12

4.  Microbial and chemical changes in chill-stored red meats.

Authors:  R H Dainty; B G Shaw; T A Roberts
Journal:  Soc Appl Bacteriol Symp Ser       Date:  1983

5.  Use of the Limulus test to determine the hygienic status of milk products as characterized by levels of gram-negative bacterial lipopolysaccharide present.

Authors:  K Hansen; T Mikkelsen; A Møller-Madsen
Journal:  J Dairy Res       Date:  1982-05       Impact factor: 1.904

6.  A numerical taxonomic study of Pseudomonas strains from spoiled meat.

Authors:  B G Shaw; J B Latty
Journal:  J Appl Bacteriol       Date:  1982-04

7.  Aerobic metabolism of Brochothrix thermosphacta growing on meat surfaces and in laboratory media.

Authors:  R H Dainty; C M Hibbard
Journal:  J Appl Bacteriol       Date:  1980-06

8.  Comparison of a gelation and a chromogenic Limulus (LAL) assay for the detection of gram-negative bacteria, and the application of the latter assay to milk.

Authors:  A Svensson; B Hahn-Hägerdal
Journal:  J Dairy Res       Date:  1987-05       Impact factor: 1.904

9.  A study of the relative incidence of different Pseudomonas groups on meat using a computer-assisted identification technique employing only carbon source tests.

Authors:  B G Shaw; J B Latty
Journal:  J Appl Bacteriol       Date:  1984-08
  9 in total
  1 in total

1.  Effect of growth temperature and pH on the aminopeptidase activity of Pseudomonas putida, P. fluorescens and Flavobacterium odoratum; the 4-nitroaniline test is reliable.

Authors:  L A Lopez-Tomas; J A Ordoñez; C Mediavilla; J L Rodriguez-Marin; P Sarmiento; A Zamora; G Garcia de Fernando
Journal:  Folia Microbiol (Praha)       Date:  2008-12-16       Impact factor: 2.099
  1 in total

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