Samaneh Bourbour1, Mohammad Emaneini1, Mahmoud Jabalameli2, Seyed Mohammad Javad Mortazavi3, Mohamad Naghi Tahmasebi3, Amirheckmat Taghizadeh4, Arash Sharafatvaziri3, Reza Beigverdi1, Fereshteh Jabalameli5. 1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2. Department of Orthopedic Surgery, Shafa Yahyaiyan Hospital, Iran University of Medical Sciences, Tehran, Iran. 3. Department of Orthopedic Surgery, Imam Khomaini Hospital, Tehran University of Medical Sciences, knee and hip surgeon, Tehran, Iran. 4. School of Electrical and Computer engineering, college of engineering, University of Tehran, Tehran, Iran. 5. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. jabalamf@tums.ac.ir.
Abstract
BACKGROUND: Accurate and rapid identification of microorganisms causing periprosthetic joint infections (PJIs) are necessary for choosing an appropriate antibiotic therapy. Therefore, molecular techniques are suggested for diagnosis in suspected PJIs. The Broad-range PCR and High-Resolution Melt Analysis (HRMA) were evaluated for the identification of causative organisms of PJIs in this study. RESULTS: For 47 of 63 specimens, both the culture and broad-range PCR were positive. The culture was found to be able of organism's detection in 74.6% (47/63) of patients. Of 47 positive cultures, 11 (23.4%) were polymicrobial and 36 (76.59%) were monomicrobial cultures, in which 34 (91.89%) cases were detected by HRM assay. The sensitivity, specificity of HRMA vs monomicrobial culture were 91.89, 93.75%, respectively. The sensitivity, specificity of total HRMA (mono + poly) vs culture were 82.92, 93.75%. CONCLUSIONS: HRM assay coupled with broad-range PCR are effective screening, rapid, and relatively cost-effective methods for discrimination of PJIs especially in aiding culture method. Using computer programs such as the Matlab-2018b program for HRM data analysis is also valuable and helpful in diagnosis.
BACKGROUND: Accurate and rapid identification of microorganisms causing periprosthetic joint infections (PJIs) are necessary for choosing an appropriate antibiotic therapy. Therefore, molecular techniques are suggested for diagnosis in suspected PJIs. The Broad-range PCR and High-Resolution Melt Analysis (HRMA) were evaluated for the identification of causative organisms of PJIs in this study. RESULTS: For 47 of 63 specimens, both the culture and broad-range PCR were positive. The culture was found to be able of organism's detection in 74.6% (47/63) of patients. Of 47 positive cultures, 11 (23.4%) were polymicrobial and 36 (76.59%) were monomicrobial cultures, in which 34 (91.89%) cases were detected by HRM assay. The sensitivity, specificity of HRMA vs monomicrobial culture were 91.89, 93.75%, respectively. The sensitivity, specificity of total HRMA (mono + poly) vs culture were 82.92, 93.75%. CONCLUSIONS: HRM assay coupled with broad-range PCR are effective screening, rapid, and relatively cost-effective methods for discrimination of PJIs especially in aiding culture method. Using computer programs such as the Matlab-2018b program for HRM data analysis is also valuable and helpful in diagnosis.
Authors: Kevin Jeng; Samuel Yang; Helen Won; Charlotte A Gaydos; Yu-Hsiang Hsieh; Alex Kecojevic; Karen C Carroll; Justin Hardick; Richard E Rothman Journal: J Clin Microbiol Date: 2012-01-11 Impact factor: 5.948
Authors: Teresa L Street; Nicholas D Sanderson; Bridget L Atkins; Andrew J Brent; Kevin Cole; Dona Foster; Martin A McNally; Sarah Oakley; Leon Peto; Adrian Taylor; Tim E A Peto; Derrick W Crook; David W Eyre Journal: J Clin Microbiol Date: 2017-05-10 Impact factor: 5.948
Authors: Marcio de Castro Ferreira; Julio Cesar Pinto Oliveira; Flavio Ferreira Zidan; Carlos Eduardo da Silveira Franciozi; Marcus Vinicius Malheiros Luzo; Rene Jorge Abdalla Journal: Rev Bras Ortop Date: 2018-06-08