Literature DB >> 22205823

Application of a 16S rRNA PCR-high-resolution melt analysis assay for rapid detection of Salmonella Bacteremia.

Kevin Jeng1, Samuel Yang, Helen Won, Charlotte A Gaydos, Yu-Hsiang Hsieh, Alex Kecojevic, Karen C Carroll, Justin Hardick, Richard E Rothman.   

Abstract

Current culture and phenotypic protocols for diagnosing Salmonella infections can be time-consuming. Here, we describe the application of a 16S rRNA PCR coupled to high-resolution melt analysis (HRMA) for species and serotype identification within 6 h of blood sample collection from a patient with Salmonella enterica serotype Enteritidis bacteremia.

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Year:  2012        PMID： 22205823      PMCID： PMC3295147          DOI： 10.1128/JCM.05121-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Quantitative detection of Salmonella enterica serovar Typhi from blood of suspected typhoid fever patients by real-time PCR.

Authors:  M Nasrum Massi; Toshiro Shirakawa; Akinobu Gotoh; Acharya Bishnu; Mochammad Hatta; Masato Kawabata
Journal:  Int J Med Microbiol       Date:  2005-06       Impact factor: 3.473

2.  Impact of reporting gram stain results from blood culture bottles on the selection of antimicrobial agents.

Authors:  Yuki Uehara; Michiko Yagoshi; Yumiko Tanimichi; Hiroko Yamada; Kazuo Shimoguchi; Sachiyo Yamamoto; Mitsuru Yanai; Kazunari Kumasaka
Journal:  Am J Clin Pathol       Date:  2009-07       Impact factor: 2.493

3.  Development of an ultra rapid and simple multiplex polymerase chain reaction technique for detection of Salmonella typhi.

Authors:  Karami Ali; Ahmadi Zeynab; Safiri Zahra; Khalilpour Akbar; Morrovati Saeid
Journal:  Saudi Med J       Date:  2006-08       Impact factor: 1.484

4.  Rapid diagnosis of typhoid fever by PCR assay using one pair of primers from flagellin gene of Salmonella typhi.

Authors:  Muhammad Nasrum Massi; Toshiro Shirakawa; Akinobu Gotoh; Acharya Bishnu; Mochammad Hatta; Masato Kawabata
Journal:  J Infect Chemother       Date:  2003-09       Impact factor: 2.211

5.  Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges.

Authors:  S E Beekmann; D J Diekema; K C Chapin; G V Doern
Journal:  J Clin Microbiol       Date:  2003-07       Impact factor: 5.948

6.  Antimicrobial therapy of septicemic patients in intensive care units before and after blood culture reporting.

Authors:  E Rintala; V Kairisto; E Eerola; J Nikoskelainen; O P Lehtonen
Journal:  Scand J Infect Dis       Date:  1991

7.  Controlled clinical comparison of the BacT/ALERT FN and the standard anaerobic SN blood culture medium.

Authors:  S Mirrett; C A Petti; C W Woods; R Magadia; M P Weinstein; L B Reller
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948

8.  FoodNet estimate of the burden of illness caused by nontyphoidal Salmonella infections in the United States.

Authors:  Andrew C Voetsch; Thomas J Van Gilder; Frederick J Angulo; Monica M Farley; Sue Shallow; Ruthanne Marcus; Paul R Cieslak; Valerie C Deneen; Robert V Tauxe
Journal:  Clin Infect Dis       Date:  2004-04-15       Impact factor: 9.079

9.  Diagnosis of typhoid fever by polymerase chain reaction.

Authors:  S R Ambati; Gopal Nath; B K Das
Journal:  Indian J Pediatr       Date:  2007-10       Impact factor: 1.967

10.  The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens.

Authors:  Tran Vu Thieu Nga; Abhilasha Karkey; Sabina Dongol; Hang Nguyen Thuy; Sarah Dunstan; Kathryn Holt; Le Thi Phuong Tu; James I Campbell; Tran Thuy Chau; Nguyen Van Vinh Chau; Amit Arjyal; Samir Koirala; Buddha Basnyat; Christiane Dolecek; Jeremy Farrar; Stephen Baker
Journal:  BMC Infect Dis       Date:  2010-05-21       Impact factor: 3.090
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  11 in total

1.  Molecular characterization of influenza viruses from women and infants in Sarlahi, Nepal.

Authors:  Jane Kuypers; Helen Y Chu; Charlotte A Gaydos; Joanne Katz; Subarna K Khatry; Steven C LeClerq; James M Tielsch; Mark C Steinhoff; Janet A Englund
Journal:  Diagn Microbiol Infect Dis       Date:  2018-11-15       Impact factor: 2.803

Review 2.  A 'culture' shift: Application of molecular techniques for diagnosing polymicrobial infections.

Authors:  Yi Zhang; Anne Hu; Nadya Andini; Samuel Yang
Journal:  Biotechnol Adv       Date:  2019-02-20       Impact factor: 14.227

3.  Sensitive detection and serovar differentiation of typhoidal and nontyphoidal Salmonella enterica species using 16S rRNA Gene PCR coupled with high-resolution melt analysis.

Authors:  Billie J Masek; Justin Hardick; Helen Won; Samuel Yang; Yu-Hsiang Hsieh; Richard E Rothman; Charlotte A Gaydos
Journal:  J Mol Diagn       Date:  2013-12-21       Impact factor: 5.568

4.  A "Culture" Shift: Broad Bacterial Detection, Identification, and Antimicrobial Susceptibility Testing Directly from Whole Blood.

Authors:  Nadya Andini; Anne Hu; Luming Zhou; Steven Cogill; Tza-Huei Wang; Carl T Wittwer; Samuel Yang
Journal:  Clin Chem       Date:  2018-08-07       Impact factor: 8.327

Review 5.  Diagnosis of Bloodstream Infections: An Evolution of Technologies towards Accurate and Rapid Identification and Antibiotic Susceptibility Testing.

Authors:  Kristel C Tjandra; Nikhil Ram-Mohan; Ryuichiro Abe; Marjan M Hashemi; Jyong-Huei Lee; Siew Mei Chin; Manuel A Roshardt; Joseph C Liao; Pak Kin Wong; Samuel Yang
Journal:  Antibiotics (Basel)       Date:  2022-04-12

6.  Trainable high resolution melt curve machine learning classifier for large-scale reliable genotyping of sequence variants.

Authors:  Pornpat Athamanolap; Vishwa Parekh; Stephanie I Fraley; Vatsal Agarwal; Dong J Shin; Michael A Jacobs; Tza-Huei Wang; Samuel Yang
Journal:  PLoS One       Date:  2014-10-02       Impact factor: 3.240

7.  Microbial Typing by Machine Learned DNA Melt Signatures.

Authors:  Nadya Andini; Bo Wang; Pornpat Athamanolap; Justin Hardick; Billie J Masek; Simone Thair; Anne Hu; Gideon Avornu; Stephen Peterson; Steven Cogill; Richard E Rothman; Karen C Carroll; Charlotte A Gaydos; Jeff Tza-Huei Wang; Serafim Batzoglou; Samuel Yang
Journal:  Sci Rep       Date:  2017-02-06       Impact factor: 4.379

8.  Massively parallel digital high resolution melt for rapid and absolutely quantitative sequence profiling.

Authors:  Daniel Ortiz Velez; Hannah Mack; Julietta Jupe; Sinead Hawker; Ninad Kulkarni; Behnam Hedayatnia; Yang Zhang; Shelley Lawrence; Stephanie I Fraley
Journal:  Sci Rep       Date:  2017-02-08       Impact factor: 4.379

9.  Nested Machine Learning Facilitates Increased Sequence Content for Large-Scale Automated High Resolution Melt Genotyping.

Authors:  Stephanie I Fraley; Pornpat Athamanolap; Billie J Masek; Justin Hardick; Karen C Carroll; Yu-Hsiang Hsieh; Richard E Rothman; Charlotte A Gaydos; Tza-Huei Wang; Samuel Yang
Journal:  Sci Rep       Date:  2016-01-18       Impact factor: 4.379

10.  Expression of DnaK and HtrA genes under high temperatures and their impact on thermotolerance of a Salmonella serotype isolated from tahini product.

Authors:  Reda M Gaafar; Marwa M Hamouda; Khalid A El-Dougdoug; Sameh Fayez Fouad
Journal:  J Genet Eng Biotechnol       Date:  2019-10-07
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