Literature DB >> 22875887

Microbiological applications of high-resolution melting analysis.

Steven Y C Tong1, Philip M Giffard.   

Abstract

High-resolution melting (HRM) analysis uses real-time PCR instrumentation to interrogate DNA sequence variation and is a low-cost, single-step, closed-tube method. Here we describe HRM technology and provide examples of varied clinical microbiological applications to highlight the strengths and limitations of HRM analysis.

Mesh：

Year:  2012        PMID： 22875887      PMCID： PMC3486245          DOI： 10.1128/JCM.01709-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  27 in total

1.  Amplicon melting analysis with labeled primers: a closed-tube method for differentiating homozygotes and heterozygotes.

Authors:  Cameron N Gundry; Joshua G Vandersteen; Gudrun H Reed; Robert J Pryor; Jian Chen; Carl T Wittwer
Journal:  Clin Chem       Date:  2003-03       Impact factor: 8.327

Review 2.  High resolution melting applications for clinical laboratory medicine.

Authors:  Maria Erali; Karl V Voelkerding; Carl T Wittwer
Journal:  Exp Mol Pathol       Date:  2008-04-13       Impact factor: 3.362

3.  Rapid, field-deployable method for genotyping and discovery of single-nucleotide polymorphisms associated with drug resistance in Plasmodium falciparum.

Authors:  Rachel Daniels; Daouda Ndiaye; Mikeal Wall; Jason McKinney; Papa Diogoye Séne; Pardis C Sabeti; Sarah K Volkman; Souleymane Mboup; Dyann F Wirth
Journal:  Antimicrob Agents Chemother       Date:  2012-03-19       Impact factor: 5.191

4.  Rapid species identification within the Mycobacterium chelonae-abscessus group by high-resolution melting analysis of hsp65 PCR products.

Authors:  Ian D Odell; Joann L Cloud; Michael Seipp; Carl T Wittwer
Journal:  Am J Clin Pathol       Date:  2005-01       Impact factor: 2.493

5.  High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni.

Authors:  Erin P Price; Helen Smith; Flavia Huygens; Philip M Giffard
Journal:  Appl Environ Microbiol       Date:  2007-03-30       Impact factor: 4.792

6.  Differentiation between vaccine and wild-type varicella-zoster virus genotypes by high-resolution melt analysis of single nucleotide polymorphisms.

Authors:  Cheryl S Toi; Dominic E Dwyer
Journal:  J Clin Virol       Date:  2008-05-13       Impact factor: 3.168

7.  High-resolution genotyping by amplicon melting analysis using LCGreen.

Authors:  Carl T Wittwer; Gudrun H Reed; Cameron N Gundry; Joshua G Vandersteen; Robert J Pryor
Journal:  Clin Chem       Date:  2003-06       Impact factor: 8.327

8.  Classification of fowl adenovirus serotypes by use of high-resolution melting-curve analysis of the hexon gene region.

Authors:  Penelope A Steer; Naomi C Kirkpatrick; Denise O'Rourke; Amir H Noormohammadi
Journal:  J Clin Microbiol       Date:  2008-11-26       Impact factor: 5.948

9.  High-resolution melting analysis of the spa repeat region of Staphylococcus aureus.

Authors:  Alex J Stephens; John Inman-Bamber; Philip M Giffard; Flavia Huygens
Journal:  Clin Chem       Date:  2008-02       Impact factor: 8.327

10.  Minim typing--a rapid and low cost MLST based typing tool for Klebsiella pneumoniae.

Authors:  Patiyan Andersson; Steven Y C Tong; Jan M Bell; John D Turnidge; Philip M Giffard
Journal:  PLoS One       Date:  2012-03-12       Impact factor: 3.240
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  44 in total

1.  Strengthening HIV surveillance: measurements to track the epidemic in real time.

Authors:  Usangiphile E Buthelezi; Candace L Davidson; Ayesha Bm Kharsany
Journal:  Afr J AIDS Res       Date:  2016-07       Impact factor: 1.300

2.  A Real-Time PCR with Melting Curve Analysis for Molecular Typing of Vibrio parahaemolyticus.

Authors:  Peiyan He; Henghui Wang; Jianyong Luo; Yong Yan; Zhongwen Chen
Journal:  Curr Microbiol       Date:  2018-05-23       Impact factor: 2.188

3.  Melting analysis on microbeads in rapid temperature-gradient inside microchannels for single nucleotide polymorphisms detection.

Authors:  Kan-Chien Li; Shih-Torng Ding; En-Chung Lin; Lon Alex Wang; Yen-Wen Lu
Journal:  Biomicrofluidics       Date:  2014-11-26       Impact factor: 2.800

4.  Metal-DNA Interactions Improve signal in High-Resolution Melting of DNA for Species Differentiation of Plasmodium Parasite.

Authors:  Priyamvada Jain; Babina Chakma; Naveen Singh; Sanjukta Patra; Pranab Goswami
Journal:  Mol Biotechnol       Date:  2017-06       Impact factor: 2.695

Review 5.  Direct detection of Strongyloides infection via molecular and antigen detection methods.

Authors:  Dinesh Balachandra; Hussain Ahmad; Norsyahida Arifin; Rahmah Noordin
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2020-07-29       Impact factor: 3.267

Review 6.  A 'culture' shift: Application of molecular techniques for diagnosing polymicrobial infections.

Authors:  Yi Zhang; Anne Hu; Nadya Andini; Samuel Yang
Journal:  Biotechnol Adv       Date:  2019-02-20       Impact factor: 14.227

7.  Rapid High-Resolution Melt Analysis of Cytauxzoon felis Cytochrome b To Aid in the Prognosis of Cytauxzoonosis.

Authors:  Megan E Schreeg; Henry S Marr; Jaime L Tarigo; Leah A Cohn; Michael G Levy; Adam J Birkenheuer
Journal:  J Clin Microbiol       Date:  2015-05-27       Impact factor: 5.948

Review 8.  Non-phenotypic tests to detect and characterize antibiotic resistance mechanisms in Enterobacteriaceae.

Authors:  Agnese Lupo; Krisztina M Papp-Wallace; Parham Sendi; Robert A Bonomo; Andrea Endimiani
Journal:  Diagn Microbiol Infect Dis       Date:  2013-10-03       Impact factor: 2.803

9.  Direct detection of macrolide resistance in Mycoplasma genitalium isolates from clinical specimens from France by use of real-time PCR and melting curve analysis.

Authors:  Arabella Touati; Olivia Peuchant; Jorgen S Jensen; Cécile Bébéar; Sabine Pereyre
Journal:  J Clin Microbiol       Date:  2014-02-26       Impact factor: 5.948

Review 10.  Recent trends in molecular diagnostics of yeast infections: from PCR to NGS.

Authors:  Toni Gabaldón
Journal:  FEMS Microbiol Rev       Date:  2019-09-01       Impact factor: 16.408
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