| Literature DB >> 33767395 |
Qiupeng Lin1,2, Shuai Jin1,2, Yuan Zong1, Hong Yu3, Zixu Zhu1,2, Guanwen Liu2,4, Liquan Kou3, Yanpeng Wang1,2, Jin-Long Qiu4,5, Jiayang Li6,7, Caixia Gao8,9.
Abstract
Prime editing (PE) applications are limited by low editing efficiency. Here we show that designing prime binding sites with a melting temperature of 30 °C leads to optimal performance in rice and that using two prime editing guide (peg) RNAs in trans encoding the same edits substantially enhances PE efficiency. Together, these approaches boost PE efficiency from 2.9-fold to 17.4-fold. Optimal pegRNAs or pegRNA pairs can be designed with our web application, PlantPegDesigner.Entities:
Year: 2021 PMID： 33767395 DOI： 10.1038/s41587-021-00868-w
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908