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Literature DB >> 35063100

CRISPR-based genome editing through the lens of DNA repair.

Tarun S Nambiar¹, Lou Baudrier², Pierre Billon³, Alberto Ciccia⁴.

Abstract

Genome editing technologies operate by inducing site-specific DNA perturbations that are resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks generated by CRISPR-associated nucleases, base modifications induced by base editors, DNA flaps created by prime editors, and integration intermediates formed by site-specific recombinases and transposases associated with CRISPR systems. Here, we discuss the cellular processes that repair CRISPR-generated DNA lesions and describe strategies to obtain desirable genomic changes through modulation of DNA repair pathways. Advances in our understanding of the DNA repair circuitry, in conjunction with the rapid development of innovative genome editing technologies, promise to greatly enhance our ability to improve food production, combat environmental pollution, develop cell-based therapies, and cure genetic and infectious diseases.

Entities: Chemical

Mesh：

Substances：

Year: 2022 PMID： 35063100 PMCID： PMC8887926 DOI： 10.1016/j.molcel.2021.12.026

Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970

471 in total

1. 53BP1 fosters fidelity of homology-directed DNA repair.

Authors: Fena Ochs; Kumar Somyajit; Matthias Altmeyer; Maj-Britt Rask; Jiri Lukas; Claudia Lukas
Journal: Nat Struct Mol Biol Date: 2016-06-27 Impact factor: 15.369

2. Fast and efficient generation of knock-in human organoids using homology-independent CRISPR-Cas9 precision genome editing.

Authors: Benedetta Artegiani; Delilah Hendriks; Joep Beumer; Rutger Kok; Xuan Zheng; Indi Joore; Susana Chuva de Sousa Lopes; Jeroen van Zon; Sander Tans; Hans Clevers
Journal: Nat Cell Biol Date: 2020-03-02 Impact factor: 28.824

CRISPR-based genome editing through the lens of DNA repair.

1. 53BP1 fosters fidelity of homology-directed DNA repair.

2. Fast and efficient generation of knock-in human organoids using homology-independent CRISPR-Cas9 precision genome editing.

Review 3. The next generation of CRISPR-Cas technologies and applications.

4. Highly efficient endogenous human gene correction using designed zinc-finger nucleases.

Review 5. Trinucleotide repeat instability during double-strand break repair: from mechanisms to gene therapy.

6. Breaking the code of DNA binding specificity of TAL-type III effectors.

Review 7. Repair of DNA Double-Strand Breaks by the Nonhomologous End Joining Pathway.

8. Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering.

9. Repurposing type I-F CRISPR-Cas system as a transcriptional activation tool in human cells.

10. Identification of pathogenic variants in cancer genes using base editing screens with editing efficiency correction.

Review 1. Therapeutic in vivo delivery of gene editing agents.

Review 2. Structural biology of CRISPR-Cas immunity and genome editing enzymes.

Review 3. Tips, Tricks, and Potential Pitfalls of CRISPR Genome Editing in Saccharomyces cerevisiae.

Review 4. The Challenges in Developing Efficient and Robust Synthetic Homing Endonuclease Gene Drives.

5. High-efficiency nonviral CRISPR/Cas9-mediated gene editing of human T cells using plasmid donor DNA.

Review 6. A Novel Anti-Cancer Therapy: CRISPR/Cas9 Gene Editing.

7. Editorial: Protecting the code: DNA double-strand break repair pathway choice.

Review 8. Nanocarriers: A novel strategy for the delivery of CRISPR/Cas systems.