Literature DB >> 33608913

Recommendations for conducting the rodent erythrocyte Pig-a assay: A report from the HESI GTTC Pig-a Workgroup.

Stephen D Dertinger1, Javed A Bhalli2, Daniel J Roberts3, Leon F Stankowski3, B Bhaskar Gollapudi4, David P Lovell5, Leslie Recio6, Takafumi Kimoto7, Daishiro Miura7, Robert H Heflich8.   

Abstract

The rodent Pig-a assay is a flow cytometric, phenotype-based method used to measure in vivo somatic cell mutation. An Organization for Economic Co-operation and Development (OECD) test guideline is currently being developed to support routine use of the assay for regulatory purposes (OECD project number 4.93). This article provides advice on best practices for designing and conducting rodent Pig-a studies in support of evaluating test substance safety, with a focus on the rat model. Various aspects of assay conduct, including laboratory proficiency, minimum number of animals per dose group, preferred treatment and blood sampling schedule, and statistical analysis are described.
© 2021 The Authors. Environmental and Molecular Mutagenesis published by Wiley Periodicals LLC on behalf of Environmental Mutagen Society.

Entities:  

Keywords:  Pig-a gene; flow cytometry; genotoxicity; mutagen; mutation assay

Mesh：

Substances：

Year:  2021        PMID： 33608913      PMCID： PMC7986863          DOI： 10.1002/em.22427

Source DB:  PubMed          Journal:  Environ Mol Mutagen        ISSN： 0893-6692            Impact factor:   3.216


  30 in total

1.  Need and potential value of the Pig-ain vivo mutation assay-a HESI perspective.

Authors:  Maik Schuler; B Bhaskar Gollapudi; Véronique Thybaud; James H Kim
Journal:  Environ Mol Mutagen       Date:  2011-10-04       Impact factor: 3.216

2.  Comparison of male versus female responses in the Pig-a mutation assay.

Authors:  Carson Labash; Svetlana L Avlasevich; Kristine Carlson; Dorothea K Torous; Ariel Berg; Jeffrey C Bemis; James T MacGregor; Stephen D Dertinger
Journal:  Mutagenesis       Date:  2015-04-01       Impact factor: 3.000

3.  3Rs friendly study designs facilitate rat liver and blood micronucleus assays and Pig-a gene mutation assessments: Proof-of-concept with 13 reference chemicals.

Authors:  Stephen D Dertinger; Svetlana L Avlasevich; Dorothea K Torous; Priyanka Singh; Sumee Khanal; Christopher Kirby; Amanda Drake; James T MacGregor; Jeffrey C Bemis
Journal:  Environ Mol Mutagen       Date:  2019-07-26       Impact factor: 3.216

4.  CD59-deficient bone marrow erythroid cells from rats treated with procarbazine and propyl-nitrosourea have mutations in the Pig-a gene.

Authors:  Javier R Revollo; Azra Dad; Mason G Pearce; Roberta A Mittelstaedt; Andrea Casildo; Rena G Lapidus; Timothy W Robison; Vasily N Dobrovolsky
Journal:  Environ Mol Mutagen       Date:  2020-08-18       Impact factor: 3.216

5.  Manifestation of Pig-a mutant bone marrow erythroids and peripheral blood erythrocytes in mice treated with N-ethyl-N-nitrosourea: direct sequencing of Pig-a cDNA from bone marrow cells negative for GPI-anchored protein expression.

Authors:  Takafumi Kimoto; Kumiko Suzuki; Xiao Mei Kobayashi; Vasily N Dobrovolsky; Robert H Heflich; Daishiro Miura; Yoshinori Kasahara
Journal:  Mutat Res       Date:  2011-04-28       Impact factor: 2.433

6.  The cloning of PIG-A, a component in the early step of GPI-anchor biosynthesis.

Authors:  T Miyata; J Takeda; Y Iida; N Yamada; N Inoue; M Takahashi; K Maeda; T Kitani; T Kinoshita
Journal:  Science       Date:  1993-02-26       Impact factor: 47.728

7.  Molecular analysis of GPI-anchor biosynthesis pathway genes in rat strains used for the Pig-a gene mutation assay.

Authors:  Azra Dad; Vasily N Dobrovolsky; Robert H Heflich; Javier R Revollo
Journal:  Mutat Res       Date:  2020-09-20       Impact factor: 2.433

8.  Standard protocol for the total red blood cell Pig-a assay used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen Society.

Authors:  Satsuki Chikura; Takafumi Kimoto; Satoru Itoh; Hisakazu Sanada; Shigeharu Muto; Katsuyoshi Horibata
Journal:  Genes Environ       Date:  2019-02-27

9.  Standard protocol for the PIGRET assay, a high-throughput reticulocyte Pig-a assay with an immunomagnetic separation, used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen and Genome Society.

Authors:  Satsuki Chikura; Takafumi Kimoto; Satoru Itoh; Hisakazu Sanada; Shigeharu Muto; Katsuyoshi Horibata
Journal:  Genes Environ       Date:  2021-03-20

10.  A network of trans-cortical capillaries as mainstay for blood circulation in long bones.

Authors:  Anika Grüneboom; Ibrahim Hawwari; Daniela Weidner; Stephan Culemann; Sylvia Müller; Sophie Henneberg; Alexandra Brenzel; Simon Merz; Lea Bornemann; Kristina Zec; Manuela Wuelling; Lasse Kling; Mike Hasenberg; Sylvia Voortmann; Stefanie Lang; Wolfgang Baum; Alexandra Ohs; Oliver Kraff; Harald H Quick; Marcus Jäger; Stefan Landgraeber; Marcel Dudda; Renzo Danuser; Jens V Stein; Manfred Rohde; Kolja Gelse; Annette I Garbe; Alexandra Adamczyk; Astrid M Westendorf; Daniel Hoffmann; Silke Christiansen; Daniel Robert Engel; Andrea Vortkamp; Gerhard Krönke; Martin Herrmann; Thomas Kamradt; Georg Schett; Anja Hasenberg; Matthias Gunzer
Journal:  Nat Metab       Date:  2019-01-21
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  1 in total

Review 1.  Recommendations for conducting the rodent erythrocyte Pig-a assay: A report from the HESI GTTC Pig-a Workgroup.

Authors:  Stephen D Dertinger; Javed A Bhalli; Daniel J Roberts; Leon F Stankowski; B Bhaskar Gollapudi; David P Lovell; Leslie Recio; Takafumi Kimoto; Daishiro Miura; Robert H Heflich
Journal:  Environ Mol Mutagen       Date:  2021-03-02       Impact factor: 3.216
  1 in total

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