| Literature DB >> 33222436 |
Kim Nguyen1, Miles Kubota1, Jon Del Arco2, Chao Feng1, Monika Singha1, Samantha Beasley1, Jasmine Sakr1, Sunil P Gandhi3, Matthew Blurton-Jones3, Jesus Fernández Lucas2,4, Robert C Spitale1,5,6.
Abstract
Profiling RNA expression in a cell-specific manner continues to be a grand challenge in biochemical research. Bioorthogonal nucleosides can be utilized to track RNA expression; however, these methods currently have limitations due to background and incorporation of analogs into undesired cells. Herein, we design and demonstrate that uracil phosphoribosyltransferase can be engineered to match 5-vinyluracil for cell-specific metabolic labeling of RNA with exceptional specificity and stringency.Entities:
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Year: 2020 PMID： 33222436 PMCID： PMC7814973 DOI： 10.1021/acschembio.0c00755
Source DB: PubMed Journal: ACS Chem Biol ISSN： 1554-8929 Impact factor: 5.100