Literature DB >> 3264406

Cytotoxic activity of an interleukin 6-Pseudomonas exotoxin fusion protein on human myeloma cells.

C B Siegall1, V K Chaudhary, D J FitzGerald, I Pastan.   

Abstract

A chimeric toxin composed of human interleukin 6 (IL-6) attached to a portion of Pseudomonas exotoxin (PE) devoid of its own cell recognition domain has been produced in Escherichia coli. The fusion protein (IL-6-PE40) is cytotoxic to a human myeloma cell line expressing IL-6 receptors but has no effect on IL-6 receptor-negative cells. The specificity of IL-6-PE40 cytotoxicity was demonstrated through competition with excess IL-6 and neutralization with an antibody to IL-6. IL-6-PE40 may be useful in the selective elimination of myeloma cells and other cells with high numbers of IL-6 receptors.

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Year:  1988        PMID： 3264406      PMCID： PMC282855          DOI： 10.1073/pnas.85.24.9738

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   12.779


  19 in total

1.  Induction of neuronal differentiation in PC12 cells by B-cell stimulatory factor 2/interleukin 6.

Authors:  T Satoh; S Nakamura; T Taga; T Matsuda; T Hirano; T Kishimoto; Y Kaziro
Journal:  Mol Cell Biol       Date:  1988-08       Impact factor: 4.272

2.  A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors:  H W Boyer; D Roulland-Dussoix
Journal:  J Mol Biol       Date:  1969-05-14       Impact factor: 5.469

Review 3.  B cell differentiation factor in synovial fluid of patients with rheumatoid arthritis.

Authors:  S Al-Balaghi; H Ström; E Möller
Journal:  Immunol Rev       Date:  1984-04       Impact factor: 12.988

4.  Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.

Authors:  F W Studier; B A Moffatt
Journal:  J Mol Biol       Date:  1986-05-05       Impact factor: 5.469

5.  Human B-cell differentiation factor defined by an anti-peptide antibody and its possible role in autoantibody production.

Authors:  T Hirano; T Taga; K Yasukawa; K Nakajima; N Nakano; F Takatsuki; M Shimizu; A Murashima; S Tsunasawa; F Sakiyama
Journal:  Proc Natl Acad Sci U S A       Date:  1987-01       Impact factor: 11.205

6.  Structure of exotoxin A of Pseudomonas aeruginosa at 3.0-Angstrom resolution.

Authors:  V S Allured; R J Collier; S F Carroll; D B McKay
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 12.779

7.  Receptors for B cell stimulatory factor 2. Quantitation, specificity, distribution, and regulation of their expression.

Authors:  T Taga; Y Kawanishi; R R Hardy; T Hirano; T Kishimoto
Journal:  J Exp Med       Date:  1987-10-01       Impact factor: 14.307

8.  Activity of a recombinant fusion protein between transforming growth factor type alpha and Pseudomonas toxin.

Authors:  V K Chaudhary; D J FitzGerald; S Adhya; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1987-07       Impact factor: 12.779

9.  Exotoxin A of Pseudomonas aeruginosa: substitution of glutamic acid 553 with aspartic acid drastically reduces toxicity and enzymatic activity.

Authors:  C M Douglas; R J Collier
Journal:  J Bacteriol       Date:  1987-11       Impact factor: 3.476

10.  Identification of the human 26-kD protein, interferon beta 2 (IFN-beta 2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor.

Authors:  J Van Damme; G Opdenakker; R J Simpson; M R Rubira; S Cayphas; A Vink; A Billiau; J Van Snick
Journal:  J Exp Med       Date:  1987-03-01       Impact factor: 14.307
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  10 in total

1.  A recombinant single-chain immunotoxin composed of anti-Tac variable regions and a truncated diphtheria toxin.

Authors:  V K Chaudhary; M G Gallo; D J FitzGerald; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

2.  A rapid method of cloning functional variable-region antibody genes in Escherichia coli as single-chain immunotoxins.

Authors:  V K Chaudhary; J K Batra; M G Gallo; M C Willingham; D J FitzGerald; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1990-02       Impact factor: 11.205

3.  Pharmacokinetics of the recombinant fusion protein DAB486IL-2 in animal models.

Authors:  P Bacha; S Forte; N Kassam; J Thomas; D Akiyoshi; C Waters; J Nichols; M Rosenblum
Journal:  Cancer Chemother Pharmacol       Date:  1990       Impact factor: 3.333

Review 4.  Interleukin-6 and its receptor during homeostasis, inflammation, and tumor growth.

Authors:  J Bauer
Journal:  Klin Wochenschr       Date:  1989-07-17

5.  Cell-specific toxicity of a chimeric protein composed of interleukin-6 and Pseudomonas exotoxin (IL6-PE40) on tumor cells.

Authors:  C B Siegall; R P Nordan; D J FitzGerald; I Pastan
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

6.  In vitro and in vivo antitumor effects of the recombinant immunotoxin IL6(T23)-PE38KDEL in multiple myeloma.

Authors:  DE-Jun Guo; Jia-Shan Han; Yan-Song Li; Zeng-Shan Liu; Shi-Ying Lu; Hong-Lin Ren
Journal:  Oncol Lett       Date:  2012-05-25       Impact factor: 2.967

Review 7.  Interleukin-6 in clinical medicine.

Authors:  J Bauer; F Herrmann
Journal:  Ann Hematol       Date:  1991-06       Impact factor: 3.673

8.  Substitution of foreign protein sequences into a chimeric toxin composed of transforming growth factor alpha and Pseudomonas exotoxin.

Authors:  W Debinski; C B Siegall; D Fitzgerald; I Pastan
Journal:  Mol Cell Biol       Date:  1991-03       Impact factor: 5.069

9.  Cytotoxic activity of a recombinant fusion protein between interleukin 4 and Pseudomonas exotoxin.

Authors:  M Ogata; V K Chaudhary; D J FitzGerald; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1989-06       Impact factor: 12.779

10.  Transforming growth factor alpha-Pseudomonas exotoxin fusion protein prolongs survival of nude mice bearing tumor xenografts.

Authors:  D C Heimbrook; S M Stirdivant; J D Ahern; N L Balishin; D R Patrick; G M Edwards; D Defeo-Jones; D J FitzGerald; I Pastan; A Oliff
Journal:  Proc Natl Acad Sci U S A       Date:  1990-06       Impact factor: 12.779
  10 in total

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