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Literature DB >> 1671711

Substitution of foreign protein sequences into a chimeric toxin composed of transforming growth factor alpha and Pseudomonas exotoxin.

W Debinski¹, C B Siegall, D Fitzgerald, I Pastan.

Abstract

TGF alpha-PE40 is a chimeric toxin made by replacing domain Ia of Pseudomonas exotoxin (PE) with transforming growth factor alpha (TGF alpha). We have now replaced a portion of domain Ib of PE with different polypeptides or an extra domain III of PE in transforming growth factor alpha-PE40 and maintained cell killing. Thus, TGF alpha-PE40 can be used to transport foreign protein sequences into the cytosol of cells.

Entities: Chemical

Mesh：

Substances：

Year: 1991 PMID： 1671711 PMCID： PMC369490 DOI： 10.1128/mcb.11.3.1751-1753.1991

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 5.069

16 in total

1. Structure and activity of diphtheria toxin. I. Thiol-dependent dissociation of a fraction of toxin into enzymically active and inactive fragments.

Authors: R J Collier; J Kandel
Journal: J Biol Chem Date: 1971-03-10 Impact factor: 5.157

2. Selective killing of HIV-infected cells by recombinant human CD4-Pseudomonas exotoxin hybrid protein.

Authors: V K Chaudhary; T Mizukami; T R Fuerst; D J FitzGerald; B Moss; I Pastan; E A Berger
Journal: Nature Date: 1988-09-22 Impact factor: 49.962

3. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.

Authors: F W Studier; B A Moffatt
Journal: J Mol Biol Date: 1986-05-05 Impact factor: 5.469

4. Cytotoxic activity of an interleukin 2-Pseudomonas exotoxin chimeric protein produced in Escherichia coli.

Authors: H Lorberboum-Galski; D FitzGerald; V Chaudhary; S Adhya; I Pastan
Journal: Proc Natl Acad Sci U S A Date: 1988-03 Impact factor: 11.205

5. Cytotoxic activities of a fusion protein comprised of TGF alpha and Pseudomonas exotoxin.

Authors: C B Siegall; Y H Xu; V K Chaudhary; S Adhya; D Fitzgerald; I Pastan
Journal: FASEB J Date: 1989-12 Impact factor: 5.834

6. Processing of Pseudomonas exotoxin by a cellular protease results in the generation of a 37,000-Da toxin fragment that is translocated to the cytosol.

Authors: M Ogata; V K Chaudhary; I Pastan; D J FitzGerald
Journal: J Biol Chem Date: 1990-11-25 Impact factor: 5.486

7. Structure of exotoxin A of Pseudomonas aeruginosa at 3.0-Angstrom resolution.

Authors: V S Allured; R J Collier; S F Carroll; D B McKay
Journal: Proc Natl Acad Sci U S A Date: 1986-03 Impact factor: 12.779

8. Activity of a recombinant fusion protein between transforming growth factor type alpha and Pseudomonas toxin.

Authors: V K Chaudhary; D J FitzGerald; S Adhya; I Pastan
Journal: Proc Natl Acad Sci U S A Date: 1987-07 Impact factor: 12.779

9. Exotoxin A of Pseudomonas aeruginosa: substitution of glutamic acid 553 with aspartic acid drastically reduces toxicity and enzymatic activity.

Authors: C M Douglas; R J Collier
Journal: J Bacteriol Date: 1987-11 Impact factor: 3.476

4. A protease-resistant immunotoxin against CD22 with greatly increased activity against CLL and diminished animal toxicity.

Authors: John E Weldon; Laiman Xiang; Oleg Chertov; Inger Margulies; Robert J Kreitman; David J FitzGerald; Ira Pastan
Journal: Blood Date: 2008-11-06 Impact factor: 25.476

4 in total