| Literature DB >> 32483365 |
Rina C Sakata1,2, Soh Ishiguro1,3,4, Hideto Mori1,3,4, Mamoru Tanaka1, Kenji Tatsuno5, Hiroki Ueda6, Shogo Yamamoto5, Motoaki Seki1, Nanami Masuyama1,3,4, Keiji Nishida7,8, Hiroshi Nishimasu9, Kazuharu Arakawa3,4,10, Akihiko Kondo7,8,11, Osamu Nureki9, Masaru Tomita1,3,4,10, Hiroyuki Aburatani5, Nozomu Yachie12,13,14,15,16,17.
Abstract
We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic targets. On-target as well as DNA and RNA off-target activities of Target-ACEmax were similar to those of existing single-function base editors.Entities:
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Year: 2020 PMID： 32483365 DOI： 10.1038/s41587-020-0509-0
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908