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Literature DB >> 32203414

The origin of genomic N⁶-methyl-deoxyadenosine in mammalian cells.

Michael U Musheev¹, Anne Baumgärtner², Laura Krebs², Christof Niehrs^3,4.

Abstract

The proposal that N6-methyl-deoxyadenosine (m6dA) acts as an epigenetic mark in mammals remains controversial. Using isotopic labeling coupled to ultrasensitive mass spectrometry, we confirm the presence of low-level m6dA in mammalian DNA. However, the bulk of genomic m6dA originates from ribo-N6-methyladenosine, which is processed via the nucleotide-salvage pathway and misincorporated by DNA polymerases. Our results argue against m6dA acting as a heritable, epigenetic DNA mark in mammalian cells.

Entities: Chemical Species

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Year: 2020 PMID： 32203414 DOI： 10.1038/s41589-020-0504-2

Source DB: PubMed Journal: Nat Chem Biol ISSN： 1552-4450 Impact factor: 15.040

1 in total

1. D(M6ATP) as a probe of the fidelity of base incorporation into polynucleotides by Escherichia coli DNA polymerase I.

Authors: J D Engel; P H von Hippel
Journal: J Biol Chem Date: 1978-02-10 Impact factor: 5.157

1 in total

20 in total

The origin of genomic N⁶-methyl-deoxyadenosine in mammalian cells.

1. D(M6ATP) as a probe of the fidelity of base incorporation into polynucleotides by Escherichia coli DNA polymerase I.

Review 1. A Role for N6-Methyladenine in DNA Damage Repair.

Review 2. Same modification, different location: the mythical role of N⁶-adenine methylation in plant genomes.

Review 3. DNA N⁶-methyldeoxyadenosine in mammals and human disease.

Review 4. DNA N6-Methyladenine Modification in Eukaryotic Genome.

Review 5. The Methylation Game: Epigenetic and Epitranscriptomic Dynamics of 5-Methylcytosine.

6. Reactivity-dependent profiling of RNA 5-methylcytidine dioxygenases.

Review 7. Means, mechanisms and consequences of adenine methylation in DNA.

8. Critical assessment of DNA adenine methylation in eukaryotes using quantitative deconvolution.

Review 9. Mapping the epigenetic modifications of DNA and RNA.

Review 10. Epigenetic Regulation in Hydra: Conserved and Divergent Roles.

The origin of genomic N6-methyl-deoxyadenosine in mammalian cells.

1. D(M6ATP) as a probe of the fidelity of base incorporation into polynucleotides by Escherichia coli DNA polymerase I.

Review 1. A Role for N6-Methyladenine in DNA Damage Repair.

Review 2. Same modification, different location: the mythical role of N6-adenine methylation in plant genomes.

Review 3. DNA N6-methyldeoxyadenosine in mammals and human disease.

Review 4. DNA N6-Methyladenine Modification in Eukaryotic Genome.

Review 5. The Methylation Game: Epigenetic and Epitranscriptomic Dynamics of 5-Methylcytosine.

6. Reactivity-dependent profiling of RNA 5-methylcytidine dioxygenases.

Review 7. Means, mechanisms and consequences of adenine methylation in DNA.

8. Critical assessment of DNA adenine methylation in eukaryotes using quantitative deconvolution.

Review 9. Mapping the epigenetic modifications of DNA and RNA.

Review 10. Epigenetic Regulation in Hydra: Conserved and Divergent Roles.

The origin of genomic N⁶-methyl-deoxyadenosine in mammalian cells.

Review 2. Same modification, different location: the mythical role of N⁶-adenine methylation in plant genomes.

Review 3. DNA N⁶-methyldeoxyadenosine in mammals and human disease.