Aspartate/asparagine-β-hydroxylase crystal structures reveal an unexpected epidermal growth factor-like domain substrate disulfide pattern.

AspH is an endoplasmic reticulum (ER) membrane-anchored 2-oxoglutarate oxygenase whose C-terminal oxygenase and tetratricopeptide repeat (TPR) domains present in the ER lumen. AspH catalyses hydroxylation of asparaginyl- and aspartyl-residues in epidermal growth factor-like domains (EGFDs). Here we report crystal structures of human AspH, with and without substrate, that reveal substantial conformational changes of the oxygenase and TPR domains during substrate binding. Fe(II)-binding by AspH is unusual, employing only two Fe(II)-binding ligands (His679/His725). Most EGFD structures adopt an established fold with a conserved Cys1-3, 2-4, 5-6 disulfide bonding pattern; an unexpected Cys3-4 disulfide bonding pattern is observed in AspH-EGFD substrate complexes, the catalytic relevance of which is supported by studies involving stable cyclic peptide substrate analogues and by effects of Ca(II) ions on activity. The results have implications for EGFD disulfide pattern processing in the ER and will enable medicinal chemistry efforts targeting human 2OG oxygenases.

Introduction

The discovery that specific asparagine- and n class="Chemical">aspartate-residues in epidermal growth factor-like domains (EGFDs) undergo hydroxylation was a landmark in post-translational modification (PTM) research, because it demonstrated proteins other than collagen type domains are directly modified by O2 (Fig. 1a)[1,2]. EGFD hydroxylation is catalysed by the aspartate/asparagine-β-hydroxylase (AspH, BAH), a non-haem ferrous iron and 2-oxoglutarate (2OG) oxygenase[3-5], which produces succinate and carbon dioxide as co-products. Human ASPH (NCBI ID: 444 [https://www.ncbi.nlm.nih.gov/gene/?term=444], AβH-J-J locus) has two promoters and undergoes extensive alternative splicing[6,7] resulting in >10 AspH isoforms (Supplementary Fig. 1). An ASPH mutation resulting in the R735W substitution correlates with Traboulsi syndrome (OMIM 601552/refSNP rs374385878), which manifests as facial dysmorphism and lens dislocation[8]; another mutation (G434V) affects human kidney function resulting in vesicoureteral reflux[9]. AspH and its truncated, likely non-catalytic, isoform Humbug are overexpressed in cancers[10-14]; AspH is strongly upregulated by hypoxia[15], consistent with its clinical use as a tumour biomarker[16,17]. Loss of murine EGFD Asp/Asn β-hydroxylation correlates with increased tumour incidence and developmental defects similar to those caused by disrupted Notch signalling[18]. AspH localises to the endoplasmic reticulum (ER) in normal cells, but (at least part of AspH) localises to the surface of tumour cells[16,17] and its hydroxylase activity was reported to enhance cell migration[19,20].

Fig. 1

EGF1 of human coagulation factor X undergoes AspH-catalysed hydroxylation at Asp103hFX. MS/MS-analysis was performed using a Bruker Daltonics amaZonTM Ion Trap LC-MS/MS system equipped with a DionexTM UltiMate® 3000 HPLC machine. Endpoint turnover assays were performed under standard (non-redox) conditions (for details see Methods section). For MS/MS-analysis, after quenching, cystine disulfides were reduced (dithiothreitol) and cysteine thiols derivatized with iodoacetamide. a Reaction scheme for diastereospecific AspH-catalysed hydroxylation of Asp/Asn-residues in EGFDs. b Schematic structure and calculated mass of the expected disulfide isomer of hFX EGF139mer (aa 86–124) featuring a canonical disulfide connectivity pattern (Cys1–3, 2–4, 5–6; green); the hydroxylation site (Asp103hFX) is in red. c His6-AspH315–758 catalysed hydroxylation of hFX EGF139mer proceeds with ~40% conversion under standard (non-redox) conditions; the light orange graph represents a control in which AspH was replaced by buffer. d LC-MS/MS analysis of the relevant digestion fragment of hFX EGF139mer (aa 101–119), which is partially hydroxylated (~40%) after treatment with His6-AspH315–758, confirms AspH-catalysed hydroxylation takes place at Asp103hFX as anticipated based on the AspH-substrate consensus sequence (fragments bearing β-hydroxy-Asp are in red). e LC-MS/MS analysis of the relevant digestion fragment of hFX EGF139mer (aa 101–119) before exposure to His6-AspH315–758

EGF1 of n class="Species">human coagulation factor X undergoes AspH-catalysed hydroxylation at Asp103hFX. MS/MS-analysis was performed using a Bruker Daltonics amaZonTM Ion Trap LC-MS/MS system equipped with a DionexTM UltiMate® 3000 HPLC machine. Endpoint turnover assays were performed under standard (non-redox) conditions (for details see Methods section). For MS/MS-analysis, after quenching, cystine disulfides were reduced (dithiothreitol) and cysteine thiols derivatized with iodoacetamide. a Reaction scheme for diastereospecific AspH-catalysed hydroxylation of Asp/Asn-residues in EGFDs. b Schematic structure and calculated mass of the expected disulfide isomer of hFX EGF139mer (aa 86–124) featuring a canonical disulfide connectivity pattern (Cys1–3, 2–4, 5–6; green); the hydroxylation site (Asp103hFX) is in red. c His6-AspH315–758 catalysed hydroxylation of hFX EGF139mer proceeds with ~40% conversion under standard (non-redox) conditions; the light orange graph represents a control in which AspH was replaced by buffer. d LC-MS/MS analysis of the relevant digestion fragment of hFX EGF139mer (aa 101–119), which is partially hydroxylated (~40%) after treatment with His6-AspH315–758, confirms AspH-catalysed hydroxylation takes place at Asp103hFX as anticipated based on the AspH-substrate consensus sequence (fragments bearing β-hydroxy-Asp are in red). e LC-MS/MS analysis of the relevant digestion fragment of hFX EGF139mer (aa 101–119) before exposure to His6-AspH315–758

EGFDs are ~40 residue domains often present in membrane protein extracellular regions; their structures are primarily comprised of β-strands stabilised by 3 conserved internal n class="Chemical">disulfides, which, as revealed by multiple crystal and NMR structures, normally adopt a thermodynamically preferred canonical Cys1–3, 2–4, 5–6 disulfide pattern[21-23]. This disulfide pattern is a functional requirement for some EGFDs[24] and for Ca(II) ion binding[25-27]. AspH catalyses (3R)-hydroxylation of specific Asp/Asn residues in EGFDs, some of which are involved in binding Ca(II) in medicinally relevant proteins, e.g. as in coagulation factors, Notch, Jagged, and Fibrillins[28,29].

There are about 60 human 2OG oxygenases with a variety of functional roles including in collagen biosynthesis, fatty acid metabolism/carnitine biosynthesis, nucleic acid repair/modification, O2 sensing, and transcriptional/translational regulation[30]. Some 2OG oxygenases are therapeutic targets, including those involved in carnitine biosynthesis and the hypoxia-inducible factor hydroxylases[31]. Structurally informed sequence alignments reveal most 2OG oxygenases possess a conserved His-Xxx-Asp/Glu…His Fe(II)-binding motif located on β-strands II and VII of the double-stranded β-helix (DSBH) core fold[32]. By contrast, sequences of AspH indicate a His-Xxx-Gly…His Fe(II)-binding motif[33], though it has been unclear if an additional residue from another part of AspH could be involved in Fe(II) binding. Despite its importance in healthy biology and cancer, few biochemical studies and no efficient substrates of isolated recombinant AspH have been reported to date.

Here, we report crystallographic and biochemical results on the structure and substrate selectivity of AspH. The results reveal a key role for the TPR domain of n class="Gene">AspH in substrate binding and, unexpectedly, that the preferred EGFD-substrates of AspH have a non-canonical (Cys3–4) disulfide pattern.

Results

AspH oxygenase domain structure

As precedented in work with other multi-domain 2OG oxygenases[34,35], we initially prepared the isolated 2OG oxygenase domain of AspH for studies on catalysis. Recombinant N-terminally hexa-His-tagged (His6) AspH562–758 (AspH-Ox) was prepared in Escherichia coli (Supplementary Fig. 2) and tested for catalytic activity using the N-terminal EGFD of human coagulation factor X (hFX EGF139mer; aa 86–124, Fig. 1b), a known cellular AspH substrate[2,36], which was prepared by solid-phase peptide synthesis followed by thiol oxidation in air-saturated buffer to give disulfides. However, no hydroxylation of hFX EGF139mer was detected by mass spectrometry (MS). Using a [1-14C]-2OG based assay, we observed AspH-Ox catalysed turnover of 2OG to CO2 in the absence of substrate, demonstrating oxygenase activity, albeit uncoupled from substrate hydroxylation as precedented with some other 2OG oxygenases under certain conditions (Supplementary Fig. 3).

To investigate the predicted unusual Fe(II)-binding motif of n class="Gene">AspH, we initiated crystallographic studies. A structure of the AspH oxygenase domain (AspH-Ox) was determined with Ni(II), substituting for Fe(II), and with L-malate (from the crystallisation buffer) bound in the predicted 2OG binding pocket (Supplementary Fig. 4). As expected, the AspH-Ox structure is comprised of 8 β-strands forming a double-stranded beta-helix (DSBH) fold, with its active site located at the more open end of the β-sandwich. In contrast to most 2OG oxygenases, the active site metal is only coordinated by two histidine residues (His679, His725), located on DSBH strands II and VII. Two water molecules and a malate molecule complete octahedral coordination of the metal ion (Supplementary Fig. 4). Overall, the Asp-Ox structure validates the expected DSBH fold with intact Fe(II) and 2OG-binding sites, consistent with the observed 2OG turnover. To further investigate the lack of EGFD substrate hydroxylation by the isolated AspH-Ox domain, we produced a longer AspH construct containing the AspH-Ox and a predicted N-terminal tetratricopeptide repeat (TPR) domain.

AspH oxygenase and TPR domain structure and activity

In contrast to AspH-Ox, the longer n class="Gene">AspH315–758 construct containing both 2OG oxygenase and TPR domains (AspH-TPR-Ox, Supplementary Fig. 5) catalysed hydroxylation of hFX EGF139mer at the anticipated Asp-residue (Asp103hFX, Fig. 1c–e). However, incomplete substrate turnover (40%) was observed even with prolonged incubation times and at higher enzyme-to-substrate ratios using hFX EGF139mer synthesized by thiol oxidation in air-saturated buffer. We next set out to obtain crystal structures of AspH-TPR-Ox, in part aiming to rationalise the observed incomplete substrate hydroxylation.

The longer AspH-TPR-Ox construct was crystallised in the presence of n class="Chemical">Mn(II), substituting for the Fe(II) co-factor, and the 2OG analogue, N-oxalylglycine (NOG); L-malate was not included in the crystallization buffer (Fig. 2 and Supplementary Fig. 6). The oxygenase domain fold conformation in the AspH-TPR-Ox structure is near identical to that of the AspH-Ox structure (main chain RMSD 0.20–0.22 Å), with the largest conformational differences occurring in an acidic loop (aa 614–620) which forms part of the active site (Supplementary Figs. 7 and 8).

Fig. 2

Crystal structure of AspH-TPR-Ox reveals Fe(II)-binding involving only two residues. Color code: grey: His6-AspH315–758; yellow: carbon-backbone of N-oxalylglycine (NOG); violet: Mn; red: oxygen; blue: nitrogen. w: water. a Topology diagram of human AspH315–758. b Overview of the AspH-TPR-Ox crystal structure. c Stereoview of the AspH-TPR-Ox crystal structure. d Close-up of the AspH-active site: Arg735 forms a salt bridge with the distal (C-5) carboxylate oxygens of NOG (2.4 and 3.1 Å) while Ser668 interacts with one oxygen lone pair through a hydrogen bond (2.7 Å). His690 is positioned to hydrogen bond to the C-1 carboxylate of NOG (2.8 Å). Mn(II) is bound to His679 (2.3 Å) and His725 (2.2 Å) of His6-AspH315–758 and coordinates two water molecules (both 2.2 Å) as well as the C-1 (2.2 Å) and C-2 (2.5 Å) carbonyl oxygens of NOG. e Representative OMIT electron density map (mFo–DFc) contoured to 3σ around NOG, Mn, water, and relevant AspH-active site residues are shown

Crystal structure of AspH-TPR-Ox reveals n class="Chemical">Fe(II)-binding involving only two residues. Color code: grey: His6-AspH315–758; yellow: carbon-backbone of N-oxalylglycine (NOG); violet: Mn; red: oxygen; blue: nitrogen. w: water. a Topology diagram of human AspH315–758. b Overview of the AspH-TPR-Ox crystal structure. c Stereoview of the AspH-TPR-Ox crystal structure. d Close-up of the AspH-active site: Arg735 forms a salt bridge with the distal (C-5) carboxylate oxygens of NOG (2.4 and 3.1 Å) while Ser668 interacts with one oxygen lone pair through a hydrogen bond (2.7 Å). His690 is positioned to hydrogen bond to the C-1 carboxylate of NOG (2.8 Å). Mn(II) is bound to His679 (2.3 Å) and His725 (2.2 Å) of His6-AspH315–758 and coordinates two water molecules (both 2.2 Å) as well as the C-1 (2.2 Å) and C-2 (2.5 Å) carbonyl oxygens of NOG. e Representative OMIT electron density map (mFo–DFc) contoured to 3σ around NOG, Mn, water, and relevant AspH-active site residues are shown

The solenoid-like TPR domain (aa 330–555) comprises 6 tandem repeating pairs of anti-parallel helices (12 helices in total, α1–α12) and is connected to the AspH-Ox domain by a hinge-loop region (aa 556–577) (Fig. 2a–c). Like that of n class="Gene">AspH-Ox, the AspH-TPR-Ox structure shows His679, His725, and two waters coordinating the metal, but instead of malate, NOG is bound in the 2OG binding pocket. The NOG C-5 carboxylate is positioned to salt bridge with Arg735 and to hydrogen-bond with the Ser668 hydroxyl (which form a “RXS motif” as observed in some other 2OG oxygenases)[31]. The NOG oxalate is bound to the active site via bidentate metal coordination and forms hydrogen bonds with the side chains of His690 and Arg688 (Fig. 2d, e). This structure indicates that the R735W substitution, present in a Traboulsi syndrome patient[8], likely interferes with 2OG binding.

The major β-sheet of the DSBH core is flanked by seven helices (α13–α19, Fig. 2a, b); the minor β-sheet is partially flanked by two helices derived from the C-terminal repeats (repeat 6) of the TPR domain. The 5 N-terminal TPR repeats form a right handed superhelix, the concave face of which partially aligns to the shorter linking loop end of repeats 2–4 that point towards the oxygenase domain and help to form the active site (Fig. 2b, c). The hinged oxygenase-TPR domain arrangement forms a large and open cavity, in which we envisaged EGFD substrates may fit. Comparison of the AspH TPR domain with other TPR domain structures identified by a DALI search reveals variations in the extent of curvature, likely due to the differences in intra-repeat interactions and loop lengths. The AspH TPR repeats 1–5 align particularly well with 5 repeats of the Candidatus magnetobacterium bavaricum magnetosome-associated (MAMA) TPR-containing protein (RMSD over 117 Cα atoms: 3.7 Å)[37]. The AspH-TPR-Ox structure suggested that the TPR domain may be directly involved in substrate binding. To investigate, we crystallized AspH-TPR-Ox in the presence of the hFX EGF139mer substrate.

AspH-TPR-Ox was successfully co-crystallized with a 39-residue fragment of hFX n class="Gene">EGF1 (AspH-TPR-Ox:hFX, Fig. 3 and Supplementary Fig. 9). The AspH-TPR-Ox structure reveals electron density at the active site corresponding to 18 of the 39 hFX EGF1 substrate residues (aa 99–116: -GKC3KGLGEYTC4TC5LEGF-) and for a disulfide linkage between Cys101hFX and Cys110hFX (Fig. 3e). Highly specific interactions, including multiple protein–protein/peptide interactions with both AspH-Ox and TPR domains are apparent (Fig. 3b–d). The N-terminal region of hFX (aa 100–105) interacts with the AspH-Ox domain and the C-terminal region of hFX (aa 106–116) interacts with the TPR domain. Comparison of AspH structures with and without substrate implies that induced fit involving substantial conformational changes during formation of the AspH-TPR-Ox:hFX protein:protein complex occurs; the distance between Leu433 on repeat 3 of the TPR domain to Pro756 of the oxygenase domain near the C-terminus is reduced from 20 to ~14 Å on substrate binding. This movement is apparently enabled by the hinge region (aa 556–577) linking the TPR and AspH-Ox domains (Fig. 4a, b).

Fig. 3

Structure of AspH-TPR-Ox:hFX features a non-canonical EGFD disulfide connectivity. Color code: magenta: carbon-backbone of NC39mer peptide; yellow: carbon-backbone of N-oxalylglycine (NOG); violet: Mn; red: oxygen; blue: nitrogen; pale yellow: sulfur. a Overview of the AspH-TPR-Ox:hFX structure; His6-AspH315–758 in grey. b Surface representation (grey) of the AspH-TPR-Ox:hFX structure showing the substrate-binding groove, His6-AspH315–758 in green. c Interactions of the TPR domain residues His493 and Arg526 as well as the hinge region residue Tyr565 with the NC39mer peptide. d Additional interactions of the TPR domain with the NC39mer peptide: Asn395 forms a hydrogen bond with Phe116hFX (3.0 Å) and Phe432 forms a hydrogen bond with Gly115hFX (3.2 Å). e OMIT electron density map (mFo–DFc) contoured to 3σ around the hFX derived NC39mer peptide supports the presence of a non-canonical disulfide bridge between Cys101hFX and Cys110hFX as a substrate requirement for AspH (canonical EGFD disulfide isomer: Cys1–3, 2–4, 5–6; non-canonical EGFD disulfide isomer: Cys1–2, 3–4, 5–6; see Fig. 5a). Note that electron density for two alternative conformation of the Asp103hFX side chain is observed

Fig. 4

Significant conformational changes occur in AspH upon substrate binding. Color code: grey: His6-AspH315–758 (AspH-TPR-Ox:hFX); turquoise: His6-AspH315–758 (AspH-TPR-Ox); magenta: carbon-backbone of NC39mer peptide; yellow: carbon-backbone of N-oxalylglycine (NOG); violet: Mn; red: oxygen; blue: nitrogen; pale yellow: sulfur. w: water. a Superimposition of the AspH-TPR-Ox:hFX (grey) and AspH-TPR-Ox (turquoise) structures indicate conformational changes in the TPR domain, the hinge region, and the oxygenase acidic loop on substrate binding. b Superimposition of the AspH-active sites of the AspH-TPR-Ox:hFX (grey) and AspH-TPR-Ox (turquoise) structures: The interaction between Glu617apo and Arg620 (2.5 Å) in the AspH-TPR-Ox structure is lost on substrate binding; both Asp616 and Glu617 interact with the substrate in the AspH-TPR-Ox:hFX structure. Gln627 (3.2 Å) and Lys666 (2.7 Å) bind to the Asp103hFX carboxylate of the active conformer of the AspH-substrate. On substrate binding, the side chain of Glu615 rotates by ~90° to interact with the side chain of Arg620 (2.8 Å) and the main chain of Leu619 (2.8 Å), rather than Lys666 (2.7 Å) as in the substrate unbound state. c The side chain of the Asp103hFX residue undergoing hydroxylation is observed in two conformations (A: magenta and B: pink; see Supplementary Fig. 10 for details). The Asp103hFX side chain carboxylate of conformation B (pink) is positioned (2.6 Å) to interact with the Mn. d Close-up of the AspH-active site: The pro-R hydrogen at the Asp103hFX β-position of the likely productive NC39mer conformation A (magenta) is positioned to interact with the Mn (distance Cβ-Mn: 4.2 Å), consistent with hydroxylation at this position

Structure of AspH-TPR-Ox:hFX features a non-canonical EGFD n class="Chemical">disulfide connectivity. Color code: magenta: carbon-backbone of NC39mer peptide; yellow: carbon-backbone of N-oxalylglycine (NOG); violet: Mn; red: oxygen; blue: nitrogen; pale yellow: sulfur. a Overview of the AspH-TPR-Ox:hFX structure; His6-AspH315–758 in grey. b Surface representation (grey) of the AspH-TPR-Ox:hFX structure showing the substrate-binding groove, His6-AspH315–758 in green. c Interactions of the TPR domain residues His493 and Arg526 as well as the hinge region residue Tyr565 with the NC39mer peptide. d Additional interactions of the TPR domain with the NC39mer peptide: Asn395 forms a hydrogen bond with Phe116hFX (3.0 Å) and Phe432 forms a hydrogen bond with Gly115hFX (3.2 Å). e OMIT electron density map (mFo–DFc) contoured to 3σ around the hFX derived NC39mer peptide supports the presence of a non-canonical disulfide bridge between Cys101hFX and Cys110hFX as a substrate requirement for AspH (canonical EGFD disulfide isomer: Cys1–3, 2–4, 5–6; non-canonical EGFD disulfide isomer: Cys1–2, 3–4, 5–6; see Fig. 5a). Note that electron density for two alternative conformation of the Asp103hFX side chain is observed

Fig. 5

AspH fully hydroxylates a mixture of canonical and non-canonical EGFD disulfides under redox conditions. End-point turnover reactions were performed as in the Methods Section. a Schematic structures of the two major hFX EGF139mer disulfide isomers identified in a single batch of hFX EGF139mer obtained by thiol oxidation in air-saturated buffer (see Supplementary Information); disulfides are in green (canonical isomer, Cys1–3, 2–4, 5–6, top; non-canonical isomer, Cys1–2, 3–4, 5–6, bottom), the hydroxylation sites (Asp103hFX) are in red. b >95% Hydroxylation of hFX EGF139mer was observed under redox conditions as opposed to partial hydroxylation under standard (non-redox) conditions (Fig. 1c), indicating that a ‘non-canonical’ EGFD-disulfide pattern (Cys 1–2, 3–4, 5–6) is the actual AspH-substrate; the light orange graph represents a control in which AspH was replaced by buffer

The side chain of Asp103hFX, the substrate n class="Chemical">asparagine undergoing hydroxylation, is at the i+2 position of a Type I β-turn adjacent to the active site metal and was modelled in two conformations (A,B; see Supplementary Fig. 10 for details), both of which are positioned to form salt bridges with the side chains of Arg688 and Lys666 (Figs. 4c and 3e). The Asp103hFX carboxylate side chain in conformation A is positioned to form a hydrogen bond (3.2 Å) with the side chain of Gln627, whilst in conformation B the carboxylate is positioned to interact with the active site metal (2.6 Å) trans to His725. Asp103hFX conformation A is poised for C-3 hydroxylation such that its pro-3R-hydrogen projects towards the metal (Cβ-metal distance ~4.5 Å) (Fig. 4d), consistent with reported (3R)-stereoselectivity for AspH catalysis[1,36]. The Asp103hFX conformation B displaces one of the two metal coordinating waters that are observed in the AspH-TPR-Ox structure without bound substrate.

Substrate binding to AspH also involves major changes within an acidic loop which forms part of the active site (aa 614–620), with the side chain n class="Chemical">carboxylates of Glu615, Asp616 and Glu617 all being shifted towards the substrate (Fig. 4b). The Glu617 side chain is positioned to form a hydrogen bond (2.8 Å) with the main chain amide nitrogen of Asp103hFX; the Asp616 side chain is positioned to form a salt bridge with the Lys102hFX side chain; and Glu615 folds into the inner apex of the acidic loop forming a salt bridge with Arg620 and a hydrogen bond with the main chain amide nitrogen of Leu619. In the AspH-Ox structure, the side chain of Glu617 appears to mimic the Asp103hFX side chain interactions with Lys666 and Arg688, possibly reflecting a function for this residue in protecting the active site from oxidation in the absence of substrate. In AspH-TPR-Ox:hFX structure, the Arg686 side chain is positioned to form a hydrogen bond with the main chain carbonyl oxygen of Lys102hFX and a salt bridge with the Asp721 side chain. The position of the side chains of Arg686, Arg688 and Asp721 in AspH-TPR-Ox:hFX does not change in comparison with AspH-TPR-Ox.

Role of the TPR domain

Notably, the highly-conn class="Chemical">served hFX tyrosine, Tyr108hFX, that is part of the consensus EGFD sequence for AspH hydroxylation[38], binds in a mostly hydrophobic pocket located between TPR repeats 5 and 6 located on the concave surface of the TPR domain. The Tyr108hFX side chain hydroxyl is positioned to form a hydrogen bond to the His493 side chain Nε2 (3.0 Å) and its main chain carbonyl to form a hydrogen bond (2.8 Å) with the Arg526 side chain. The Tyr108hFX side chain is apparently positioned to form skewed π-π-stacking interactions with the side chains of Phe496 and Arg526. The main chain nitrogen of Cys110hFX (2.9 Å) interacts with the Tyr565 side chain (Fig. 3c).

At the hFX C-terminus, the phenyl ring of Phe116hFX is buried in a hydrophobic pocket on the TPR domain formed by Ala389, Leu398, and Phe432, which are located between TPR repeats 2 and 3. The phenyl ring of Phe116hFX is positioned to π-stack with the Arg393 guanidine side chain and the peptide bonds linking Arg393 with Ser394 and Asn395 (Fig. 3d). The Phe116hFX main chain amide NH forms a hydrogen bond with the Asn395 side chain Oδ1 (2.6 Å). Sequence alignment of known hydroxylated EGFDs shows that the aromatic residues at the Phe116hFX position are highly conserved (Supplementary Fig. 11).

Unexpectedly, in the AspH-TPR-Ox substrate complex we obn class="Chemical">served a non-canonical disulfide bridge, i.e. between Cys101hFX and Cys110hFX for the hFX substrate, in effect forming a ten-residue ring (Fig. 3e). The substrate disulfide is positioned in a hydrophobic pocket formed by Tyr565, Pro682, and Ile758. This pocket is shared with Leu105hFX and expanded by Phe529 and Leu564 of AspH.

The observed hFX n class="Chemical">disulfide corresponds to a non-canonical Cys3–4 EGFD disulfide link, suggesting this pattern is required for AspH catalysis rather than the established (Cys1–3, 2–4, 5–6) disulfide pattern. The canonical EGFD disulfide pattern (Cys1–3, 2–4, 5–6) has been observed in all reported crystal and NMR structures of EGFDs bearing the AspH-substrate consensus sequence, including for hFX[39,40]. A non-canonical disulfide pattern (Cys1–2, 3–4, 5–6) has been reported in crystalline and solution state studies on the EGFD-5 of human thrombomodulin[41-43]; this, however, does not have the AspH substrate consensus sequence. MS[44] and computational[45] work also suggests the possibility of a non-canonical disulfide connectivity pattern for EGFDs bearing the consensus sequence for AspH-catalysed hydroxylation. It was, therefore, important to investigate AspH activity towards disulfide patterns with the non-canonical 3–4 link (i.e. Cys1–2, 3–4, 5–6; 1–5, 3–4, 2–6; or 1–6, 2–5, 3–4).

AspH accepts an unexpected EGFD disulfide pattern

MS analyses imply that the n class="Chemical">disulfide pattern arising from thiol oxidation of the linearly synthesised hFX EGF139mer in air-saturated buffer predominantly comprises a mixture of the canonical (Cys1–3, 2–4, 5–6) and non-canonical (Cys1–2, 3–4, 5–6) disulfide patterns (Fig. 5a); other additional disulfide isomers may be present in the mixture but were not detected. The canonical and non-canonical disulfide isomers co-elute as a single peak in MS coupled HPLC (Supplementary Figs. 12 and 13). Similar results have been reported for the synthesis of EGFD5 of human thrombomodulin[46]. The hFX EGF139mer mixture underwent partial hydroxylation (~40%, Fig. 1c) by AspH-TPR-Ox under normal assay conditions. However, it underwent complete hydroxylation (>95%) when incubated with enzyme in a redox buffer containing a mixture of reduced and oxidized glutathione (GSH and GSSG). The conditions were employed to enable reversible thiol-disulfide interchange of the canonical and non-canonical hFX forms (Fig. 5b).

AspH fully n class="Chemical">hydroxylates a mixture of canonical and non-canonical EGFD disulfides under redox conditions. End-point turnover reactions were performed as in the Methods Section. a Schematic structures of the two major hFX EGF139mer disulfide isomers identified in a single batch of hFX EGF139mer obtained by thiol oxidation in air-saturated buffer (see Supplementary Information); disulfides are in green (canonical isomer, Cys1–3, 2–4, 5–6, top; non-canonical isomer, Cys1–2, 3–4, 5–6, bottom), the hydroxylation sites (Asp103hFX) are in red. b >95% Hydroxylation of hFX EGF139mer was observed under redox conditions as opposed to partial hydroxylation under standard (non-redox) conditions (Fig. 1c), indicating that a ‘non-canonical’ EGFD-disulfide pattern (Cys 1–2, 3–4, 5–6) is the actual AspH-substrate; the light orange graph represents a control in which AspH was replaced by buffer

The complexities of the reversible nature of thiol-disulfide interchange in solution render definitive identification of the preferred n class="Chemical">disulfide pattern of the AspH EGFD substrate difficult. We thus synthesised an irreversibly-linked cyclic analogue of the Cys3–4 EGFD disulfide substrate; A 10mer cyclic peptide (CP101–110) comprising the core residues (aa 101–110) of hFX EGF139mer, wherein the Cys3–4 disulfide was replaced by a stable thioether, was prepared using the method of Suga et al.[47,48]. CP101–110 was efficiently Asp-β-hydroxylated by AspH-TPR-Ox (~85%, Supplementary Figs. 14a and 15), whereas cyclic peptides with variations in consensus sequence residues or a linear 10mer peptide were not (Supplementary Fig. 14), further supporting the crystallographically observed Cys3–4 disulfide as the preferred substrate form for AspH catalysis. An extended 19mer CP (CP101–119, Fig. 6a) was designed based on the enzyme-substrate contacts observed in the AspH-TPR-Ox:hFX structure and found to be a better substrate than CP101–110 (>95%, Fig. 6b). A structure of AspH co-crystallised with the CP101–119 (AspH-TPR-Ox:CP101–119) reveals this substrate bound to one of the two AspH molecules in the asymmetric unit (chain A). Conformational differences between the bound (chain A) and unbound (chain B) (Cα RMSD 3.82 Å) are consistent with afore-described observations implying an AspH induced fit mechanism with hFX (Fig. 6c and Supplementary Fig. 16).

Fig. 6

A cyclic peptide mimicking the non-canonical Cys3–4 EGFD is an excellent AspH-substrate. End-point turnover reactions were performed as in the Methods Section. a Schematic structure and calculated mass of the cyclic peptide CP101–119 mimicking the central macrocyclic disulfide of the non-canonical isomer of hFX EGF139mer (Cys3–4); the hydroxylation site (Asp103hfX) is in red. b >95% Hydroxylation of the CP101–119 peptide was observed under standard (non-redox) conditions; the light orange graph represents a control in which AspH was replaced by buffer. c Two His6-AspH315–758-molecules are present in the asymmetric unit of the AspH-TPR-Ox:CP101–119 crystal structure, only one binds the CP101–119 peptide (details in the Supplementary Information): The superimposition of the two independent AspH-molecules of this crystal structure (colour code: unbound AspH: raspberry; AspH bound to CP101–119: grey; CP101–119: green) highlights an induced fit mechanism of AspH upon substrate binding: Major conformational changes occur in the TPR domain, the hinge region and the acidic loop whilst the overall oxygenase domain conformation is hardly affected. The thioether linker of the cyclic peptide could not be accurately modelled into the electron density; however, complementary analytical methods imply the presence of a cyclic thioether linkage (see Supplementary Figure 16 for further details)

A cyclic peptide mimicking the non-canonical n class="Chemical">Cys3–4 EGFD is an excellent AspH-substrate. End-point turnover reactions were performed as in the Methods Section. a Schematic structure and calculated mass of the cyclic peptide CP101–119 mimicking the central macrocyclic disulfide of the non-canonical isomer of hFX EGF139mer (Cys3–4); the hydroxylation site (Asp103hfX) is in red. b >95% Hydroxylation of the CP101–119 peptide was observed under standard (non-redox) conditions; the light orange graph represents a control in which AspH was replaced by buffer. c Two His6-AspH315–758-molecules are present in the asymmetric unit of the AspH-TPR-Ox:CP101–119 crystal structure, only one binds the CP101–119 peptide (details in the Supplementary Information): The superimposition of the two independent AspH-molecules of this crystal structure (colour code: unbound AspH: raspberry; AspH bound to CP101–119: grey; CP101–119: green) highlights an induced fit mechanism of AspH upon substrate binding: Major conformational changes occur in the TPR domain, the hinge region and the acidic loop whilst the overall oxygenase domain conformation is hardly affected. The thioether linker of the cyclic peptide could not be accurately modelled into the electron density; however, complementary analytical methods imply the presence of a cyclic thioether linkage (see Supplementary Figure 16 for further details)

Substrate variant analysis

Next, we assayed n class="Gene">AspH for its ability to hydroxylate truncated 26mer hFX EGF1 substrates (aa 86–111) lacking the Cys5–6 disulfide-forming residues, synthesized using orthogonal cysteine protection to yield selectively both the pure canonical (C26mer, Fig. 7a) or non-canonical (NC26mer, Fig. 7d) disulfide isomers. Only the NC26mer peptide was hydroxylated under normal assay conditions (Fig. 7b, e), whereas both 26mer peptides were hydroxylated using redox buffer conditions (Fig. 7c, f). A structure of AspH in complex with NC26mer was obtained (AspH-TPR-Ox:NC26mer, Supplementary Fig. 17). Comparison of AspH-TPR-Ox:NC26mer and AspH-TPR-Ox:hFX structures reveals little overall difference (Cα RMSD 0.13 Å). The presence of a Cys3–4 disulfide bond indicates that the additional residues present in the longer 39mer substrate, which were not observed in electron density maps of AspH-TPR-Ox:NC26mer due to disorder, are likely not essential for substrate recognition. The efficient hydroxylation of the synthetic hFX EGF139mer with cysteines 1, 2, 5 and 6 (aa 90, 95, 112 and 121, respectively) all being replaced by serines (NC-4Ser39mer) further supports the proposal that only the substrate Cys3–4 disulfide is required for AspH-catalysed hydroxylation (Fig. 8a–c). A structure of AspH in complex with the NC-4Ser39mer substrate was obtained (AspH-TPR-Ox:NC-Ser39mer, Supplementary Fig. 18). Comparison of the AspH-TPR-Ox:NC-Ser39mer and AspH-TPR-Ox:hFX structures reveals little overall difference (Cα RMSD 0.08 Å, Fig. 8d), including for the Ser112NC-Ser39mer and Cys112hFX residues, suggesting that Cys112hFX may not necessarily form a disulfide during productive AspH-catalysis.

Fig. 7

The EGFD disulfide connectivity determines the degree of AspH-catalysed hydroxylation under non-redox conditions. End-point turnover reactions were performed as in the Methods Section. Light orange graphs represent controls in which AspH was replaced by buffer. a Schematic structure and calculated mass of the C26mer peptide, bearing a canonical disulfide arrangement (Cys1–3, 2–4); disulfides are shown in green, the hydroxylation site (Asp103hFX) is indicated in red. b No hydroxylation of the C26mer peptide was observed under standard (non-redox) conditions; ~7% impurity (might correspond to an oxidized byproduct) was observed in this sample, including the control samples. c >95% Hydroxylation of the C26mer peptide was observed under redox conditions. d Schematic structure and calculated mass of the NC26mer peptide, bearing a non-canonical disulfide arrangement (Cys1–2, 3–4); disulfides are in green, the hydroxylation site (Asp103hFX) is in red. e >95% Hydroxylation of the NC26mer peptide was observed under standard (non-redox) conditions. f ~83% Hydroxylation of the NC26mer peptide was observed under redox conditions

Fig. 8

The AspH-substrate requirement is a ring composed of 10 amino acid residues. End-point turnover reactions were performed as in the Methods Section. Light orange graphs represent controls in which AspH was replaced by buffer. a Schematic structure and calculated mass of the NC-4Ser39mer peptide featuring a single disulfide between Cys3–4 (green); its sequence is identical to the one of hFX EGF139mer (see Fig. 1a), except that Cys90hFX, 95, 112, 121 are substituted for Ser (light blue) to avoid disulfide scrambling; the hydroxylation site (Asp103hFX) is in red. b >95% Hydroxylation was observed under standard (non-redox) conditions. c >95% Hydroxylation was observed under redox conditions. d Superimposition of the AspH-TPR-Ox:hFX crystal structure (color code: AspH: grey; NC39mer peptide: magenta) with the AspH-TPR-Ox:NC-Ser39mer crystal structure (color code: AspH: gold; NC-4Ser39mer peptide: slate blue) shows a high conservation of the conformations of both enzyme and ligands

The EGFD disulfide connectivity determines the degree of n class="Gene">AspH-catalysed hydroxylation under non-redox conditions. End-point turnover reactions were performed as in the Methods Section. Light orange graphs represent controls in which AspH was replaced by buffer. a Schematic structure and calculated mass of the C26mer peptide, bearing a canonical disulfide arrangement (Cys1–3, 2–4); disulfides are shown in green, the hydroxylation site (Asp103hFX) is indicated in red. b No hydroxylation of the C26mer peptide was observed under standard (non-redox) conditions; ~7% impurity (might correspond to an oxidized byproduct) was observed in this sample, including the control samples. c >95% Hydroxylation of the C26mer peptide was observed under redox conditions. d Schematic structure and calculated mass of the NC26mer peptide, bearing a non-canonical disulfide arrangement (Cys1–2, 3–4); disulfides are in green, the hydroxylation site (Asp103hFX) is in red. e >95% Hydroxylation of the NC26mer peptide was observed under standard (non-redox) conditions. f ~83% Hydroxylation of the NC26mer peptide was observed under redox conditions

The AspH-substrate requirement is a ring composed of 10 amino acid residues. End-point turnover reactions were performed as in the Methods Section. Light orange graphs represent controls in which n class="Gene">AspH was replaced by buffer. a Schematic structure and calculated mass of the NC-4Ser39mer peptide featuring a single disulfide between Cys3–4 (green); its sequence is identical to the one of hFX EGF139mer (see Fig. 1a), except that Cys90hFX, 95, 112, 121 are substituted for Ser (light blue) to avoid disulfide scrambling; the hydroxylation site (Asp103hFX) is in red. b >95% Hydroxylation was observed under standard (non-redox) conditions. c >95% Hydroxylation was observed under redox conditions. d Superimposition of the AspH-TPR-Ox:hFX crystal structure (color code: AspH: grey; NC39mer peptide: magenta) with the AspH-TPR-Ox:NC-Ser39mer crystal structure (color code: AspH: gold; NC-4Ser39mer peptide: slate blue) shows a high conservation of the conformations of both enzyme and ligands

AspH with other EGFD protein substrates

To investigate the generality of the hFX results, we tested recombinant Ca(II)-binding EGFDs (cbEGFDs) (~10–15 kDa) from n class="Species">human Fibrillin-1 (hFib1); a two EGFD containing construct, cbEGF32-33[49], a three EGFD construct, cbEGF41-43[50], and the single EGFD construct, TB4cbEGF23 (with a cbEGFD preceded by a transforming growth factor β-binding protein-like (TB) domain)[51], and a three EGFD construct, cbEGF11-13, of Notch-1 (hNotch1)[52]. All these were hydroxylated by AspH-TPR-Ox (30–85%) under redox buffer conditions as determined by MS after proteolytic digestion, but were much poorer substrates under non-redox conditions (0–30%) (Supplementary Fig. 19).

Effect of calcium ions on EFGD stability and AspH activity

As most AspH-substrate EGFDs bear a n class="Gene">Ca(II)-binding site nearby the AspH-hydroxylation site[28], we next investigated if Ca(II) ions inhibit EGFD hydroxylation, i.e. by stabilising the canonical EGFD fold which is apparently not an AspH-substrate. No effect on AspH-TPR-Ox activity was observed on addition of 3 mM Ca(II) ions with any of the tested substrates in the absence of redox buffer. By contrast, in the presence of redox buffer, hydroxylase activity was not significantly affected for the recombinant multi-domain proteins hFib1 (cbEGF32–33 and cbEGF41–43) and hNotch 1 (cbEGF11–13). However, AspH hydroxylase activity was substantially inhibited for the recombinant hFib1 fragment, TB4cbEGF23 (aa 1527–1647, Fig. 9a), which binds Ca(II) tightly (Kd = 16 ± 1 nM)[51] and which is a poor AspH substrate under non-redox hydroxylation conditions (<5% hydroxylation, Fig. 9b), but an efficient one in redox buffer (~85% hydroxylation, Fig. 9c). The addition of Ca(II) ions at 1 mM final concentration in redox buffer reduced the extent of TB4cbEGF23 hydroxylation from ~85 to ~25%; in the presence of 3 mM Ca(II) ions in redox buffer TB4cbEGF23 hydroxylation was further reduced to ~10% (Fig. 9d, e). No inhibitory effect was observed when 3 mM Mg(II) was added instead of Ca(II) (Supplementary Fig. 20a). Ca(II) ions do not directly inhibit AspH, as the irreversibly formed cyclic thioether, CP2101–110, was fully hydroxylated in the presence of 10 mM Ca(II) (Supplementary Fig. 20b).

Fig. 9

Ca(II) ions inhibit AspH-catalysed hydroxylation of a human fibrillin-1 fragment. End-point turnover reactions were performed with an incubation time of 180 min (See Methods Section). Prior to analysis, the substrate was reduced and cysteine thiols were derivatized with N-ethylmaleimide, then digested using trypsin, and analysed using MALDI-ToF-MS. Light orange graphs represent controls in which AspH was replaced by buffer. a Sequence of the fragment of human fibrillin-1, TB4cbEGF23 (hFIB1_TB4cbEGF23, aa 1527–1647; Uniprot Database entry: FBN1_HUMAN), bearing the hydroxylation site (Asn1624hFIB1, red). b <5% Hydroxylation was observed under standard (non-redox) conditions. c ~85% Hydroxylation was observed under redox conditions. d ~25% Hydroxylation was observed under redox conditions in the presence of 1 mM Ca(II). e ~10% Hydroxylation was observed under redox conditions in the presence of 3 mM Ca(II)

Ca(II) ions inhibit n class="Gene">AspH-catalysed hydroxylation of a human fibrillin-1 fragment. End-point turnover reactions were performed with an incubation time of 180 min (See Methods Section). Prior to analysis, the substrate was reduced and cysteine thiols were derivatized with N-ethylmaleimide, then digested using trypsin, and analysed using MALDI-ToF-MS. Light orange graphs represent controls in which AspH was replaced by buffer. a Sequence of the fragment of human fibrillin-1, TB4cbEGF23 (hFIB1_TB4cbEGF23, aa 1527–1647; Uniprot Database entry: FBN1_HUMAN), bearing the hydroxylation site (Asn1624hFIB1, red). b <5% Hydroxylation was observed under standard (non-redox) conditions. c ~85% Hydroxylation was observed under redox conditions. d ~25% Hydroxylation was observed under redox conditions in the presence of 1 mM Ca(II). e ~10% Hydroxylation was observed under redox conditions in the presence of 3 mM Ca(II)

Since Ca(II) ions preferably bind to and stabilise the canonical EGFD fold[27], these results further support assignment of the non-canonical 3–4 EGFD n class="Chemical">disulfide form as a preferred AspH substrate. The effects of the neighbouring domains and the different intrinsic Ca(II) affinities of EGFD proteins complicates the understanding of the influence of Ca(II) ions on AspH-catalysis. Nonetheless, the results clearly reveal the potential for regulation of AspH activity by Ca(II) ions by stabilisation of cbEGFDs in the canonical disulfide pattern fold, so making them unavailable for disulfide shuffling to the non-canonical Cys3–4 pattern.

Discussion

There is an unmet need for further investigations into the biological functions of human n class="Gene">AspH and its role in genetic diseases and cancer. Such work is challenging given the membrane bound nature of AspH[5], the presence of multiple different isoforms/splice variants (>10 in humans)[6,7], its location in the ER[5], and the redox sensitive nature of its disulfide substrates[53]. Our work defining the first substrates for isolated AspH and the roles for the TPR domain in its catalysis provides a structural basis for future work on the cellular and physiological roles of AspH.

Whilst the oxygenase domain fold of n class="Gene">AspH is typical for the 2OG oxygenase superfamily (Fig. 2), details of its active site and the role of the TPR domain in EGFD-substrate binding are remarkable. Key insights arising from our structures include: the presence of an active site metal bound by only two protein ligands, the role of the TPR domain in substrate binding, and the fact that AspH only accepts EGFD-substrates with the non-canonical Cys3–4, instead of the canonical Cys1–3, 2–4, 5–6, disulfide pattern. The latter finding was unexpected given the prevalence of the canonical EGFD disulfide pattern (at least outside of the ER) reported in the literature[54]. The lack of in vitro hydroxylation assays for AspH has hindered progress on its characterisation; our results will enable further detailed kinetic, mechanistic, and inhibition studies on AspH.

Comparison of the AspH structures with and without substrates implies substantial induced fit during substrate binding (Fig. 4a, b); experience with n class="Chemical">NMR studies on single oxygenase domain 2OG oxygenases[55-57] implies that in the case of AspH, even more substantial changes in conformation may occur in solution, likely involving both oxygenase and TPR domains, as well as AspH-substrates. Whatever the precise extent of conformational changes during catalysis, the results reveal a central role for the TPR domain in enabling AspH catalysis—without it, 2OG turnover occurs, but EGFDs are not hydroxylated (Fig. 4 and Supplementary Fig. 3).

TPR domains are involved in protein-protein interactions and substrate recognition in other proteins/enzymes[58] including human 2OG oxygenases, i.e. the procollagen C-4 (CP4Hs) and C-3 (Leprecans) prolyl- and C-5 (PLODs) lysyl-hydroxylases, which contain (or are predicted to contain) TPR domains N-terminal to the oxygenase domain[59]. As yet, there are no structures reported for the oxygenase domains of eukaryotic members of these TPR containing enzymes, which like AspH are ER localised, though the TPR domain of CPH in complex with a collagen-like peptide has been structurally characterised[60]. Our work reveals how the AspH oxygenase and TPR domains recognise and bind a non-canonical 3–4 disulfide EGFD and, through conformational changes to the enzyme, guide and position the Asp/Asn residue to be hydroxylated to the active site in a catalytically productive manner. It would seem probable that some of the TPR domains in other ER-localised 2OG oxygenases have analogous roles.

The role of the TPR domain in AspH catan class="Chemical">lysis is precedented in the roles of some non-catalytic domains in substrate targeting by the 2OG dependent JmjC Nε-methyl lysine histone demethylases (KDMs), though this is only understood in detail from a structural perspective for two KDM7 subfamily members (KDM7A/B)[61]. Binding of a highly-conserved EGFD tyrosine (e.g. Tyr108hFX) to a hydrophobic pocket on the TPR domain surface is reminiscent of H3K4me3 binding to the plant homeobox domain (PHD) of KDM7A[61]. Analogous recognition domains to that of KDM7A/B occur in other JmjC KDMs, involving different domain types (e.g. Tudor domains), though to our knowledge TPR domains are not present in the chromatin modifying 2OG oxygenases; they thus appear to be characteristic of the ER localised 2OG oxygenases.

The combined results reveal that AspH accen class="Chemical">pts EGFD substrates with a non-canonical Cys3–4 disulfide pattern, rather than the canonical Cys1–3, 2–4, 5–6 pattern. Evidence for this comes from structural data, MS turnover assays under different redox conditions, inhibition by Ca(II) ions (which presumably bind and stabilise the canonical but not the non-canonical disulfide pattern), and the use of a stable disulfide cyclic peptide analogue. These observations are interesting because they raise the possibility of EGFDs manifesting dynamic disulfide patterns in the redox active environment of the ER, rather than being limited to the canonical form normally observed by in vitro biophysics and which current evidence implies would likely be dominating in the oxidising extracellular environment. Indeed, the combined crystallographic evidence suggests the canonical form is the most thermodynamically stable[21-23]. AspH could act as a chaperone regulating EGFD folding or recognising misfolded EGFDs, possibly as part of the redox sensitive unfolded protein response (UPR) pathway[62]. This is consistent with the identification of various degrees of β-hydroxylation in purified native proteins containing EGFDs, most notably human coagulation proteins hFIX (30%) and hFX (100%)[63], which implies they have been through a folding cycle prior to secretion into the bloodstream. Interestingly, purified hFVII does not show any β-hydroxylation, despite having the AspH-substrate consensus sequence, which suggests a possible role of AspH in recognising misfolded rather than functionally active folded EGFDs. One role of AspH could be to regulate EGFD folding with hydroxylation being an oxygen sensitive element of this process rather than an end in itself; this proposal is consistent with the lack of observed effect of Asn/Asp hydroxylation on the canonical EGFD fold conformation and on Ca(II) ion binding[63]. Extracellular AspH could play a role in recognising and labelling mis-folded EGFDs on the cell surface, possibly in a manner relating to over-production of AspH in cancer cells[10,11,13,16,17], since the latter can have dysregulated EGFD proteins involved in signalling (e.g. Notch)[64,65]. The structures reported here may also of be interest with respect to defining factors involved in AspH localisation, in particular to the surfaces of some tumour cells[16,17]. They raise the possibility of AspH localisation being regulated by EGFD binding in a manner dependent on the EGFD disulfide pattern and redox environment. The results also suggest that AspH activity may be regulated by Ca(II) ions; in this regard it is interesting that there are predicted Ca(II) binding EF-hand motifs in human AspH itself.

The results clearly reveal that, at least under the analysed conditions, n class="Gene">AspH has an unusual Fe(II) coordination chemistry for a 2OG-dependent hydroxylase (Fig. 2d, e). It should be noted that all the AspH crystal structures presented here are in complex with Ni(II) or Mn(II) and a 2OG mimetic, i.e. NOG or L-malate. We considered the possibility that in the presence of Fe(II) and 2OG a third protein-derived ligand interacts with the metal. However, this scenario  seems unlikely given that the metal coordination geometries and substrate positions observed in many other 2OG-dependent oxygenase crystal structures in complex with Fe(II) and 2OG compared to Ni(II)/Mn(II) and NOG are very similar, with no evidence for additional enzyme derived ligands being observed[31].

The reason why AspH employs two- rather than three-protein derived metal ligands is unclear, but sequence comparisons suggest this is a highly-conserved feature (Supplementary Fig. 6). It also appears to be linked to other characteristic active site features involving 2OG and substrate binding, which may compensate for the lack of a third protein ligand. It is of interest that in the structure of AspH-TPR-Ox complexed with a 39-residue fragment of hFX EGF1 (AspH-TPR-Ox:hFX, Fig. 4) the Asp residue positioned for AspH-catalysed hydroxylation is observed in two conformations. One conformer is apparently positioned for stereoselective hydroxylation in a manner precedented for other 2OG oxygenases (Supplementary Fig. 21). By contrast, the Asp side chain carboxylate of the other conformer is positioned to coordinate with the active site metal (Fig. 4). Whilst the latter conformation may be involved in catalysis, it may be that within the endoplasmic reticulum, AspH substrates are involved in maintaining Fe(II) at its active site.

The unusual coordination chemistry of AspH may relate to an as yet undetermined physiological or disease role as it is the case for the 2OG dependent n class="Disease">hypoxia-inducible factor (HIF) prolyl hydroxylases (PHDs), where it is proposed that the unusual kinetic properties of PHDs, i.e. their slow reaction with O2, both reflect their active site chemistry and roles as hypoxia sensors[31]. Given the unusual coordination chemistry of AspH, detailed kinetic and solution biophysical studies to investigate dioxygen binding and water ligand displacement are of considerable interest and are the subject of ongoing work.

We compared the AspH structures with the n class="Species">human HIF Asn-hydroxylase, factor inhibiting HIF (FIH, PDB: 1H2K)[66], and a prokaryotic asparagine hydroxylase, AsnO (PDB: 2OG7)[67] (Supplementary Fig. 21). Analysis of enzyme-substrate complex structures reveals similar substrate side chain orientations at the active sites of AsnO and FIH, consistent with knowledge that they produce the same (3S)-stereochemistry products[67,68], which contrasts with the (3R)-product of AspH[1,36]. AsnO has an active site arginine (Arg305AsnO), which interacts with the Cα carboxylate of the asparagine substrate, similarly to the interaction of AspH Arg688 with the side chain of Asp103hFX (Supplementary Fig. 20). However, Arg305AsnO is located on β-strand VIII and AspH Arg688 is located on β-strand III of their respective DSBHs, possibly reflecting convergent evolution within the 2OG oxygenase superfamily. Interestingly, FIH also interacts with the side chain of its Asn (and Asp) substrates using an arginine, Arg238FIH, but one which is located on an insert linking DSBH β-strands βIV-V (Supplementary Fig. 21). Biochemical and structural studies on FIH metal binding carboxylate variants D201A and D201G reveal coordination by only two His residues and that the D201G variant is catalytically viable[69]. Comparison of these structures with AspH shows the main chain Cα of the carboxylate ligand positions are very similar, but that the hFX substrate residue side chain likely causes changes in metal geometry. It is probable that second sphere residues, including the substrate side chain, contribute to catalysis in the two-His metal binding 2OG oxygenases. 2OG dependent halogenases (SyrB2/CytC3/WelO5/AmbO5) also have two His metal binding ligands (His-Xxx-Ala…His motif), an arrangement enabling halide coordination to the active site metal[70]. We have not observed halogenase activity with AspH in the presence of chloride ions, implying other factors in addition to the number of metal ligands control substrate hydroxylation versus halogenation.

The ability to carry out assays with isolated AspH and knowledge of its active site and substrate binding mode provide a platform from which to develop small molecule n class="Gene">AspH inhibitors. These will be useful in dissecting its biological functions and will help to enable new cancer treatments and the development of selective inhibitors for other human 2OG oxygenases, some of which are current medicinal chemistry targets[71].

Methods

Recombinant protein production and purification

A pET-28a(+) vector encoding for N-terminally His6-tagged n class="Gene">AspH315–758 was transformed into E. coli BL21 (DE3) cells. Cells were grown in 2TY media supplemented with kanamycin (0.05 mM; 30 mg/ml) at 37 °C with shaking (180 rpm). AspH production was induced by adding isopropyl ß-D-thiogalactopyranoside to a final concentration of 0.1 mM when the OD600 reached 1.2 at 18 °C. Cells were shaken for 16 h at 18 °C, then harvested by centrifugation (10,967 × g, 8 min, 4 °C); the resultant cell pellets were stored at –80 °C. The frozen cell pellets were resuspended in ice-cold (30 g/100 mL) 50 mM HEPES buffer (pH 7.5, 500 mM NaCl, 5 mM imidazole) containing EDTA-free protease inhibitor cocktail tablets (1 tablet/50 mL, Roche Diagnostics) and DNAse I (bovine pancreas, grade II, Roche Diagnostics). Cells were lysed by sonication on ice (8 × 30 s bursts; Sonics Vibra-Cell VCX500, amplitude: 60%) and the lysate was centrifuged (48,384 × g, 30 min, 4 °C). The supernatant containing AspH was purified at 4 °C using Ni(II)-affinity chromatography (HisTrap HP column, GE Healthcare) on an ÄKTA Pure machine (GE Healthcare) with a step elution gradient (to 50 mM HEPES, pH 7.5, 500 mM NaCl, 40 mM imidazole) and elution buffers (50 mM HEPES, pH 7.5, 500 mM NaCl, 500 mM imidazole; AspH typically eluted at around 150-200 mM imidazole). Eluted fractions containing AspH were pooled, concentrated using Amicon Ultra centrifugal filters (3082 × g, 4 °C), and further purified by size-exclusion chromatography using a HiLoad 26/60 Superdex 75 pg 300 mL column with a flow rate of 1 mL/min and 50 mM HEPES (pH 7.5, 500 mM NaCl) as elution buffer. The protein was subsequently concentrated and buffer exchanged into 50 mM HEPES, pH 7.5 for storage at −80 °C until further use. Purity of the proteins was assessed by SDS-PAGE and MS (Supplementary Fig. 5).

AspH-substrates

A 39mer peptide based on the amino acid sequence of the first EGFD of hFX (hFX n class="Gene">EGF139mer, aa 86–124) was synthesized by solid phase peptide synthesis (SPPS) and purified by Peptide Synthetics (Peptide Protein Research Ltd, UK). Regioselective disulfide formation to yield exclusively (within our analytical limits) the canonical disulfide pattern (Cys1–3, 2–4, 5–6) was achieved using a sequence of orthogonal cysteine protection and selective cysteine oxidation[72]: However, no hydroxylation of this peptide was observed when exposed to His6-AspH315–758 and cofactors. Therefore, the 39mer peptide was resynthesized with the disulfide bridges being formed by thiol-oxidation in air-saturated buffer. This method has previously been applied to synthesize bovine FX[4] and FIX[24] peptides that were successfully hydroxylated by bovine AspH. Using this protocol, a mixture of the canonical (Cys1–3, 2–4, 5–6; C39mer) and non-canonical (Cys1–2, 3–4, 5–6; NC39mer) disulfide-isomers was obtained (Supplementary Fig. 12 and 13), which was used in all subsequent hydroxylation assays. Derivatives of hFX EGF139mer containing either canonical (C26mer) or non-canonical (NC26mer; NC-4Ser39mer) disulfides were synthesized as single disulfide isomers by GL Biochem (Shanghai) Ltd using orthogonal cysteine protection. The Ca(II)-binding EGFD-containing multi-domain AspH-substrates (hFIB1_cbEGFD32–33[49], hFIB1_cbEGFD41–43[50], hFIB1_TB4cbEGF23[51], hNotch1_cbEGFD11–13[52]) were produced recombinantly in E. coli and purified as reported in the cited literature.

Design and synthesis of cyclic peptides

Based on the AspH-TPR-Ox:hFX crystal structure, stable cyclic pen class="Chemical">ptides comprising the core ring residues (aa 101–110) of the hFX EGF139mer-substrate with a thioether replacing the Cys3–4 disulfide were synthesized. The D-stereochemistry of the N-terminal amino acid (aa 101) aligns the peptide side chain with the main chain of the original substrate.

Commercial Fmoc-protected amino acids (AGTC Bioproducts; Alfa Aesar; CSBio; Iris Biotech; Novabiochem; Sigma-Aldrich; n class="Gene">TCI) were used as received. SPPS was performed using a CSBio CS336X automated peptide synthesizer following standard Fmoc-strategy: linear peptides were synthesized from the C- to N-termini on a 0.1 mmol scale using a Rink amide linker using N,N-diisopropylcarbodiimide/1-hydroxybenzotriazole for coupling.

To obtain cyclic peptides, the n class="Chemical">N-terminal Fmoc-protecting group was cleaved on the resin, and the resin suspended in DMF (4 mL) containing N-chloroacetylsuccinimide[73] (150 mg). The mixture was gently shaken for 3 h, filtered, and the dried resin treated with a solution of trifluoroacetic acid, triisopropylsilane, and water (4 mL; 95/2.5/2.5). After 3 h, the mixture was filtered and the solution diluted with 45 mL cold diethyl ether. The suspension was centrifuged (4000 rpm, 10 min, 4 °C), decanted, and then taken up in 1.5 mL aqueous triethylammonium acetate buffer (1 M, pH 8.5, pH readjusted with trimethylamine) and heated for 10 min at 100 °C in a microwave reactor (Biotage Initiator). Directly afterwards, the crude cyclic peptides were purified by semi-preparative HPLC (DionexTM UltiMate® 3000, Thermo Scientific) using a reverse phase column (Grace Vydac® 218TP101522) and a gradient of acetonitrile in milliQ water (each containing 0.1%v/v trifluoroacetic acid) specified in Supplementary Fig. 14.

AspH activity assays

All reagents were obtained from commercial sources (Alfa Aesar; Sigma-Aldrich; TCI). For all turn-over experiments, His6-tagged n class="Gene">AspH-constructs were used, as no effect upon cleavage of the His6-tag was observed in initial activity assays. Hydroxylation assays were initialised by mixing a solution containing AspH (10 μM), L-ascorbic acid (400 μM), and (NH4)2Fe(SO4)2·7H2O (50 μM) in the appropriate buffer with a solution containing substrate (100 μM), disodium 2-oxoglutarate (300 μM), and, if desired, additional reagents (CaCl2, EGTA) in the appropriate buffer. The buffers used comprised: Standard, non-redox, Buffer (50 mM HEPES pH 7.5, 150 mM NaCl); Redox Buffer (50 mM Tris, pH 8.5, 3.0 mM reduced L-glutathione, 0.3 mM oxidized L-glutathione, 150 mM NaCl); Refolding Buffer (50 mM Tris, pH 8.5, 3.0 mM L-cysteine, 0.3 mM L-cystine, 10 mM CaCl2). Reactions were performed at 37 °C for 60 min unless otherwise noted, and then quenched by the addition of an equal volume of 1%v/v aqueous formic acid. All assays were performed in triplicate alongside an additional no-enzyme control (His6-AspH substituted by buffer).

The hydroxylase activity of n class="Gene">AspH was assayed by MALDI-ToF MS in the positive ion reflectron mode (Bruker Daltonics Ultraflex I machine with 32–60% laser energy; Waters Micromass MALDI micro MX with flight tube voltage: 12 kV, reflectron voltage: 5.2 kV, laser fire rate: 10 Hz, pulse voltage: 1950 V, detector voltage: 2750 V): the relative quantities of substrate and hydroxylated product were analysed with respect to no-enzyme controls. A 1:4 volume ratio of sample to MALDI-matrix was used for sample preparation (20 mg/mL 2,5-dihydroxybenzoic acid, 10 mg/mL α-cyano-4-hydroxycinnamic acid or 10 mg/mL sinapinic acid in aqueous 50% CH3CN containing 0.1%v/v  trifluoroacetic acid). For the disulfide containing substrates a matrix composed of a 10:1 mixture of 10 mg/mL α-cyano-4-hydroxycinnamic acid:10 mg/mL 2-(4′-hydroxybenzeneazo)benzoic acid in aqueous 50%v/v CH3CN containing 0.1%v/v trifluoroacetic acid was used. Mass spectra were analysed using MassLynx software (Version 4.1). Apart from the AspH-substrate peaks, peaks corresponding to the desired AspH-substrate mass reduced by 18 Da are frequently observed in the MALDI-MS spectra; these peaks result from the ionization induced liberation of H2O upon intramolecular cyclization of aspartates to form aspartyl succinimides (−18 Da)[74] and are taken into account when integrating the peaks to determine conversions.

Crystallography

Crystals of N-terminally His6-tagged n class="Gene">AspH562–758 were grown by the vapour diffusion method at 20 °C in 150 nL sitting drops. The drops were prepared by mixing 100 nL of protein solution (9.5 mg/mL containing 1 mM N-oxalylglycine (NOG) and 10 mM NiSO4) and 50 nL of precipitant (50 mM disodium malate, 30%v/v PEG-3350, 30% dextran sulfate sodium salt) with a protein to well ratio of 1:2. Crystals were cryo-cooled in liquid N2 with 25% glycerol as cryoprotectant and data were collected at 100 K using synchroton radiation at the Diamond Light Source (DLS).

For the remaining structures, high-throughput crystallization screens were set up using a Phoenix RE liquid dispensing robot (Art Robbins Instruments) on 96-well, 3-subwell low profile Intelliplates (Art Robbins Instruments) using Hampton Research Molecular Dimensions crystallization screens (JCSG plus, n class="Gene">PACT, INDEX, PEG/Ion, Structure, Salt, MIDAS) or optimization screens. N-Terminally His6-tagged AspH315–758 (18 mg/mL in 50 mM HEPES buffer, pH 7.5) was mixed with 1 mM MnCl2 (pH 7.5), 2 mM N-oxalylglycine (pH 7.5), and, when appropriate, an AspH-substrate (see Supplementary Table 1 for details). Crystals were grown by the vapour diffusion method at 4 °C in 200 or 300 nL sitting drops using 2:1, 1:1 or 1:2 sample:well solution ratios. Crystals were cryoprotected using mother liquor supplemented with 25%v/v glycerol before cryo-cooling in liquid N2. Data were collected at 100 K at DLS. Data were indexed, integrated, and scaled using HKL-3000[75], XDS[76], SCALA[77], Xia2[78] or CrystalClear (Rigaku). Detailed crystallization conditions for all crystal structures and a summary of data collection parameters are given in Supplementary Table 1.

Structure solution and refinements

The structure of AspH-Ox was determined by single isomorphous replacement with anomalous scattering (SIRAS). Briefly, n class="Gene">AspH crystals were derivatised by incubating in a reservoir solution supplemented with 10 mM K2PtCl4 for 5 h. Crystals were transferred to and backsoaked for several seconds in a reservoir solution supplemented with 25% ethylene glycol instead of Pt(II) and crystals were immediately cryo-cooled in liquid N2.

The AspH-Ox (native and n class="Chemical">Pt-derivatised) datasets were integrated (XDS[76]) and subsequently scaled (SCALA). SHELXD[79] was used to locate three platinum positions in the Pt-dataset. Heavy atom positions were refined and initial phases calculated with SHARP[80] using the SIRAS method. The final electron density map after phase extension and solvent flattening with SOLOMON[81] was of excellent quality and automated model building with ARP/wARP[82] resulted in a 95% complete model. Refinement with PHENIX[83] and several rounds of manual rebuilding in COOT[84] resulted in a model with a final Rfactor/Rfree of 15.3/18.6%, respectively.

The AspH-TPR-Ox structure was determined by molecular replacement (MR) using the AutoMR (PHAn class="Chemical">SER[85]) subroutine in PHENIX. The coordinates of the AspH oxygenase domain (His6-tagged AspH562–758 as a search model, PDB: 5APA) were first used to successfully identify a rotation and translation solution for the oxygenase domain. Initial attempts to find a rotation and translation solution for the TPR using the C-terminal TPR domain of peroxisomal targeting signal 1 receptor (PEX5)[86] were complicated by the repeating nature of the TPR fold. In order to overcome problems associated with the repeating fold the search model was trimmed to include three sequential repeats (PEX5 Gln300-Tyr395, PDB: 1FCH). The 3 C-terminal TPR repeats of AspH were identified in a search after the oxygenase domain was fixed. This allowed manual fitting of 3 further TPR repeats to the N-terminus of AspH. Rigid body refinement of the three separate fragments followed by simulated annealing refinement (both cartesian and torsion) led to a model with Rfree 42.15. Iterative rounds of model building and fitting in COOT[84] and refinement in PHENIX[83] were performed until the decreasing R and Rfree no longer converged and a final model was obtained. Due to domain movements between different crystal forms and complexes, all other structures were determined by MR using PHASER with the oxygenase and TPR domains searched for independently. The oxygenase domain was always searched first followed by the TPR domain. For data from crystals grown in the presence of substrate, up to 18 residues of bound substrate were modelled. Refinement statistics are given in Supplementary Table 2. Representative electron density (with contour level and map types defined in legends) is shown in Figs. 2 and 3 and in Supplementary Figs. 4, 7, 16–18.