Robert V Williams1,2, Jeong-Yeh Yang2, Kelley W Moremen2,3, I Jonathan Amster1, James H Prestegard4,5,6. 1. Department of Chemistry, University of Georgia, Athens, GA, USA. 2. Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA. 3. Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA. 4. Department of Chemistry, University of Georgia, Athens, GA, USA. jpresteg@ccrc.uga.edu. 5. Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA, USA. jpresteg@ccrc.uga.edu. 6. Complex Carbohydrate Research Center, University of Georgia, Athens, GA, USA. jpresteg@ccrc.uga.edu.
Abstract
Residual dipolar couplings (RDCs) provide both structural and dynamical information useful in the characterization of biological macromolecules. While most data come from the interaction of simple pairs of directly bonded spin-1/2 nuclei (1H-15N, 1H-13C, 1H-1H), it is possible to acquire data from interactions among the multiple spins of 13C-labeled methyl groups (1H3-13C). This is especially important because of the advantages that observation of 13C-labeled methyl groups offers in working with very large molecules. Here we consider some of the options for measurement of methyl RDCs in large and often fully protonated proteins and arrive at a pulse sequence that exploits both J-modulation and direct detection of 13C. Its utility is illustrated by application to a fully protonated two domain fragment from the mammalian glycoprotein, Robo1, 13C-methyl-labeled in all valines.
Residual dipolar couplings (RDCs) provide both structural and dynamical information useful in the characterization of biological macromolecules. While most data come from the interaction of simple pairs of directly bonded spin-1/2 nuclei (n class="Chemical">1H-15N, 1H-13C, 1H-1H), it is possible to acquire data from interactions among the multiple spins of 13C-labeled methyl groups (1H3-13C). This is especially important because of the advantages that observation of 13C-labeled methyl groups offers in working with very large molecules. Here we consider some of the options for measurement of methyl RDCs in large and often fully protonated proteins and arrive at a pulse sequence that exploits both J-modulation and direct detection of 13C. Its utility is illustrated by application to a fully protonated two domain fragment from the mammalian glycoprotein, Robo1, 13C-methyl-labeled in all valines.
Authors: Robert Creutznacher; Thorben Maass; Jasmin Dülfer; Clara Feldmann; Veronika Hartmann; Miranda Sophie Lane; Jan Knickmann; Leon Torben Westermann; Lars Thiede; Thomas J Smith; Charlotte Uetrecht; Alvaro Mallagaray; Christopher A Waudby; Stefan Taube; Thomas Peters Journal: Commun Biol Date: 2022-06-09