Literature DB >> 11430758

Multiplet component separation for measurement of methyl 13C-1H dipolar couplings in weakly aligned proteins.

G Kontaxis1, A Bax.   

Abstract

A simple spectral editing procedure is described that generates separate subspectra for the methyl 13C-[1H3] multiplet components of 1H-13C HSQC spectra. The editing procedure relies on co-addition of in-phase and antiphase spectra and yields 1H-coupled constant-time HSQC subspectra for the methyl region that have the simplicity of the regular decoupled CT-HSQC spectrum. Resulting spectra permit rapid and reliable measurement of 1H-13C J and dipolar couplings. The editing procedure is illustrated for a Ca2+-calmodulin sample in isotropic and liquid crystalline phases.

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Year:  2001        PMID： 11430758     DOI： 10.1023/a:1011280529850

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  20 in total

1.  The use of dipolar couplings for determining the solution structure of rat apo-S100B(betabeta).

Authors:  A C Drohat; N Tjandra; D M Baldisseri; D J Weber
Journal:  Protein Sci       Date:  1999-04       Impact factor: 6.725

2.  Protein side-chain rotamers from dipolar couplings in a liquid crystalline phase.

Authors:  J J Chou; A Bax
Journal:  J Am Chem Soc       Date:  2001-04-25       Impact factor: 15.419

3.  Measurement of homonuclear proton couplings from regular 2D COSY spectra.

Authors:  F Delaglio; Z Wu; A Bax
Journal:  J Magn Reson       Date:  2001-04       Impact factor: 2.229

4.  Integration of spin-state-selective excitation into 2D NMR correlation experiments with the heteronuclear ZQ/2Q pi rotations for 1JXH- resolved E.COSY-type measurements of heteronuclear coupling constants in proteins.

Authors:  A Meissner; J O Duus; O W Sørensen
Journal:  J Biomol NMR       Date:  1997-07       Impact factor: 2.835

Review 5.  Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra.

Authors:  M Ottiger; F Delaglio; A Bax
Journal:  J Magn Reson       Date:  1998-04       Impact factor: 2.229

6.  Measurement of dipolar contributions to 1JCH splittings from magnetic-field dependence of J modulation in two-dimensional NMR spectra.

Authors:  N Tjandra; A Bax
Journal:  J Magn Reson       Date:  1997-02       Impact factor: 2.229

7.  NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors:  F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal:  J Biomol NMR       Date:  1995-11       Impact factor: 2.835

Review 8.  Pulsed-field gradients: theory and practice.

Authors:  J Keeler; R T Clowes; A L Davis; E D Laue
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

9.  Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.

Authors:  M Piotto; V Saudek; V Sklenár
Journal:  J Biomol NMR       Date:  1992-11       Impact factor: 2.835

10.  Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution.

Authors:  J R Tolman; J M Flanagan; M A Kennedy; J H Prestegard
Journal:  Proc Natl Acad Sci U S A       Date:  1995-09-26       Impact factor: 11.205
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  16 in total

1.  Ensembles of a small number of conformations with relative populations.

Authors:  Vijay Vammi; Guang Song
Journal:  J Biomol NMR       Date:  2015-10-17       Impact factor: 2.835

2.  Accurate determination of leucine and valine side-chain conformations using U-[15N/13C/2H]/[1H-(methine/methyl)-Leu/Val] isotope labeling, NOE pattern recognition, and methine Cgamma-Hgamma/Cbeta-Hbeta residual dipolar couplings: application to the 34-kDa enzyme IIA(chitobiose).

Authors:  Chun Tang; Junji Iwahara; G Marius Clore
Journal:  J Biomol NMR       Date:  2005-10       Impact factor: 2.835

3.  Measurement of eight scalar and dipolar couplings for methine-methylene pairs in proteins and nucleic acids.

Authors:  Emeric Miclet; Jérôme Boisbouvier; Ad Bax
Journal:  J Biomol NMR       Date:  2005-03       Impact factor: 2.835

4.  Simultaneous alpha/beta spin-state selection for (13)C and (15)N from a time-shared HSQC-IPAP experiment.

Authors:  Pau Nolis; Teodor Parella
Journal:  J Biomol NMR       Date:  2006-12-12       Impact factor: 2.835

5.  Improved accuracy in measuring one-bond and two-bond (15)N, (13)C (α) coupling constants in proteins by double-inphase/antiphase (DIPAP) spectroscopy.

Authors:  Frank Löhr; Sina Reckel; Susanne Stefer; Volker Dötsch; Jürgen M Schmidt
Journal:  J Biomol NMR       Date:  2011-06-07       Impact factor: 2.835

6.  Enhancing the quality of protein conformation ensembles with relative populations.

Authors:  Vijay Vammi; Tu-Liang Lin; Guang Song
Journal:  J Biomol NMR       Date:  2014-02-12       Impact factor: 2.835

7.  Probing the Broad Time Scale and Heterogeneous Conformational Dynamics in the Catalytic Core of the Arf-GAP ASAP1 via Methyl Adiabatic Relaxation Dispersion.

Authors:  Fa-An Chao; Yifei Li; Yue Zhang; R Andrew Byrd
Journal:  J Am Chem Soc       Date:  2019-07-22       Impact factor: 15.419

8.  A spin-state-selective experiment for measuring heteronuclear one-bond and homonuclear two-bond couplings from an HSQC-type spectrum.

Authors:  Perttu Permi
Journal:  J Biomol NMR       Date:  2002-01       Impact factor: 2.835

9.  Unfolding the mechanism of the AAA+ unfoldase VAT by a combined cryo-EM, solution NMR study.

Authors:  Rui Huang; Zev A Ripstein; Rafal Augustyniak; Michal Lazniewski; Krzysztof Ginalski; Lewis E Kay; John L Rubinstein
Journal:  Proc Natl Acad Sci U S A       Date:  2016-07-11       Impact factor: 11.205

10.  Measurement of residual dipolar couplings from 1Halpha to 13Calpha and 15N using a simple HNCA-based experiment.

Authors:  Perttu Permi
Journal:  J Biomol NMR       Date:  2003-12       Impact factor: 2.835
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