Literature DB >> 3098169

DNA hybridization probe for the Pseudomonas fluorescens group.

H Festl, W Ludwig, K H Schleifer.   

Abstract

Plasmid pHF360 was constructed from cloned rRNA genes (rDNA) of Pseudomonas aeruginosa and used as hybridization probe for the Pseudomonas fluorescens group. The probe was tested by dot and in situ colony hybridizations to chromosomal DNAs from a wide variety of organisms. pHF360 DNA hybridized exclusively to chromosomal DNAs from bacteria representing the P. fluorescens group and separated them clearly from all other bacteria tested in the present study. Determination of the nucleotide sequence of the cloned DNA showed that it is a fragment from a 23S rRNA gene of P. aeruginosa. It was compared with the published 23S RNA sequence from Escherichia coli.

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Year:  1986        PMID： 3098169      PMCID： PMC239196          DOI： 10.1128/aem.52.5.1190-1194.1986

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  18 in total

1.  Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors:  M Grunstein; D S Hogness
Journal:  Proc Natl Acad Sci U S A       Date:  1975-10       Impact factor: 11.205

2.  A phylogenetic definition of the major eubacterial taxa.

Authors:  C R Woese; E Stackebrandt; T J Macke; G E Fox
Journal:  Syst Appl Microbiol       Date:  1985       Impact factor: 4.022

3.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

4.  Characterization of saccharolytic nonfermentative bacteria associated with man.

Authors:  M J Pickett; M M Pedersen
Journal:  Can J Microbiol       Date:  1970-05       Impact factor: 2.419

5.  Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli.

Authors:  J Brosius; T J Dull; H F Noller
Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205

6.  Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.

Authors:  F C Kafatos; C W Jones; A Efstratiadis
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

7.  Cellular fatty acids and metabolic products of Pseudomonas species obtained from clinical specimens.

Authors:  C W Moss; S B Dees
Journal:  J Clin Microbiol       Date:  1976-12       Impact factor: 5.948

8.  A novel cloning vector for the direct selection of recombinant DNA in E. coli.

Authors:  H Hennecke; I Günther; F Binder
Journal:  Gene       Date:  1982-09       Impact factor: 3.688

9.  Molecular epidemiology of Vibrio cholerae in the U.S. Gulf Coast.

Authors:  J B Kaper; H B Bradford; N C Roberts; S Falkow
Journal:  J Clin Microbiol       Date:  1982-07       Impact factor: 5.948

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  15 in total

1.  Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes.

Authors:  D Betzl; W Ludwig; K H Schleifer
Journal:  Appl Environ Microbiol       Date:  1990-09       Impact factor: 4.792

2.  Identification of mesophilic lactic acid bacteria by using polymerase chain reaction-amplified variable regions of 16S rRNA and specific DNA probes.

Authors:  N Klijn; A H Weerkamp; W M de Vos
Journal:  Appl Environ Microbiol       Date:  1991-11       Impact factor: 4.792

3.  Catabolic plasmids of environmental and ecological significance.

Authors:  G S Sayler; S W Hooper; A C Layton; J M King
Journal:  Microb Ecol       Date:  1990-01       Impact factor: 4.552

4.  Comparison of Nucleic Acid Hybridization and Fluorometry for Measurement of the Relationship between RNA/DNA Ratio and Growth Rate in a Marine Bacterium.

Authors:  L Kerkhof; B B Ward
Journal:  Appl Environ Microbiol       Date:  1993-05       Impact factor: 4.792

5.  Transport of a genetically engineered Pseudomonas fluorescens strain through a soil microcosm.

Authors:  J T Trevors; J D van Elsas; L S van Overbeek; M E Starodub
Journal:  Appl Environ Microbiol       Date:  1990-02       Impact factor: 4.792

6.  Identification of Vibrio anguillarum in fish by using partial 16S rRNA sequences and a specific 16S rRNA oligonucleotide probe.

Authors:  A S Rehnstam; A Norqvist; H Wolf-Watz; A Hagström
Journal:  Appl Environ Microbiol       Date:  1989-08       Impact factor: 4.792

7.  nodZ, a unique host-specific nodulation gene, is involved in the fucosylation of the lipooligosaccharide nodulation signal of Bradyrhizobium japonicum.

Authors:  G Stacey; S Luka; J Sanjuan; Z Banfalvi; A J Nieuwkoop; J Y Chun; L S Forsberg; R Carlson
Journal:  J Bacteriol       Date:  1994-02       Impact factor: 3.490

8.  Identification and characterization of a novel Bradyrhizobium japonicum gene involved in host-specific nitrogen fixation.

Authors:  J Y Chun; G L Sexton; L E Roth; G Stacey
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

9.  Nitrification and Denitrification: Probing the Nitrogen Cycle in Aquatic Environments

Authors: 
Journal:  Microb Ecol       Date:  1996-11       Impact factor: 4.552

10.  A species-specific probe and a PCR assay for the marine bacterium, Pseudomonas stutzeri strain Zobell.

Authors:  L Kerkhof
Journal:  Microb Ecol       Date:  1994-05       Impact factor: 4.552
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