Literature DB >> 6293932

A novel cloning vector for the direct selection of recombinant DNA in E. coli.

H Hennecke, I Günther, F Binder.   

Abstract

A new plasmid cloning vector (pHE3) is described carrying dominant p-fluorophenylalanine-sensitivity (pheS) and chloramphenicol-resistance markers. This vector is used in combination with a p-fluorophenylalanine-resistant Escherichia coli recipient (strain RR28). Foreign DNA can be cloned into pHE3 leading to insertional inactivation of pheS. Transformation of RR28, and plating on minimal medium with chloramphenicol plus p-fluorophenylalanine (pfp) directly selects for colonies containing recombinant DNA.

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Year:  1982        PMID： 6293932     DOI： 10.1016/0378-1119(82)90011-7

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  39 in total

1.  Overproduction of an acetylxylan esterase from the extreme thermophile "Caldocellum saccharolyticum" in Escherichia coli.

Authors:  E Lüthi; N B Jasmat; P L Bergquist
Journal:  Appl Microbiol Biotechnol       Date:  1990-11       Impact factor: 4.813

2.  Complete nucleotide sequences of seven eubacterial genes coding for the elongation factor Tu: functional, structural and phylogenetic evaluations.

Authors:  W Ludwig; M Weizenegger; D Betzl; E Leidel; T Lenz; A Ludvigsen; D Möllenhoff; P Wenzig; K H Schleifer
Journal:  Arch Microbiol       Date:  1990       Impact factor: 2.552

3.  The molecular organization of the lysostaphin gene and its sequences repeated in tandem.

Authors:  P Heinrich; R Rosenstein; M Böhmer; P Sonner; F Götz
Journal:  Mol Gen Genet       Date:  1987-10

4.  Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for a beta-mannanase from the extremely thermophilic bacterium "Caldocellum saccharolyticum".

Authors:  E Lüthi; N B Jasmat; R A Grayling; D R Love; P L Bergquist
Journal:  Appl Environ Microbiol       Date:  1991-03       Impact factor: 4.792

5.  From one gene to two proteins: the biogenesis of cytochromes b and c1 in Bradyrhizobium japonicum.

Authors:  L Thöny-Meyer; P James; H Hennecke
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-01       Impact factor: 11.205

6.  DNA hybridization probe for the Pseudomonas fluorescens group.

Authors:  H Festl; W Ludwig; K H Schleifer
Journal:  Appl Environ Microbiol       Date:  1986-11       Impact factor: 4.792

7.  Insertion and deletion mutations within the nif region of Rhizobium japonicum.

Authors:  M Hahn; L Meyer; D Studer; B Regensburger; H Hennecke
Journal:  Plant Mol Biol       Date:  1984-05       Impact factor: 4.076

8.  Isolation and characterization of insertion sequence elements from gram-negative bacteria by using new broad-host-range, positive selection vectors.

Authors:  R Simon; B Hötte; B Klauke; B Kosier
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490

9.  Rhizobium japonicum nitrogenase Fe protein gene (nifH).

Authors:  M Fuhrmann; H Hennecke
Journal:  J Bacteriol       Date:  1984-06       Impact factor: 3.490

10.  Biosynthesis of riboflavin: cloning, sequencing, mapping, and expression of the gene coding for GTP cyclohydrolase II in Escherichia coli.

Authors:  G Richter; H Ritz; G Katzenmeier; R Volk; A Kohnle; F Lottspeich; D Allendorf; A Bacher
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490
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