| Literature DB >> 30792304 |
Shuichi Hoshika1,2, Nicole A Leal1,2, Myong-Jung Kim1,2, Myong-Sang Kim1, Nilesh B Karalkar1,2, Hyo-Joong Kim1, Alison M Bates3, Norman E Watkins4, Holly A SantaLucia4, Adam J Meyer5, Saurja DasGupta6, Joseph A Piccirilli6, Andrew D Ellington5, John SantaLucia4, Millie M Georgiadis3, Steven A Benner7,2.
Abstract
We report DNA- and RNA-like systems built from eight nucleotide "letters" (hence the name "hachimoji") that form four orthogonal pairs. These synthetic systems meet the structural requirements needed to support Darwinian evolution, including a polyelectrolyte backbone, predictable thermodynamic stability, and stereoregular building blocks that fit a Schrödinger aperiodic crystal. Measured thermodynamic parameters predict the stability of hachimoji duplexes, allowing hachimoji DNA to increase the information density of natural terran DNA. Three crystal structures show that the synthetic building blocks do not perturb the aperiodic crystal seen in the DNA double helix. Hachimoji DNA was then transcribed to give hachimoji RNA in the form of a functioning fluorescent hachimoji aptamer. These results expand the scope of molecular structures that might support life, including life throughout the cosmos.Entities:
Mesh：
Substances：
Year: 2019 PMID： 30792304 PMCID： PMC6413494 DOI： 10.1126/science.aat0971
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728