| Literature DB >> 30531939 |
Masaki Endo1, Masafumi Mikami1,2, Akira Endo1,3, Hidetaka Kaya1,4, Takeshi Itoh5, Hiroshi Nishimasu6, Osamu Nureki6, Seiichi Toki7,8,9.
Abstract
Streptococcus pyogenes Cas9 (SpCas9) is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1) can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.Entities:
Mesh：
Substances：
Year: 2018 PMID： 30531939 DOI： 10.1038/s41477-018-0321-8
Source DB: PubMed Journal: Nat Plants ISSN： 2055-0278 Impact factor: 15.793