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Literature DB >> 649568

Biochemical and genetic properties of site-specific restriction endonucleases in Bacillus globigii.

Abstract

Bacillus globigii contains two site-specific endonucleases, BPGLI AND BglI. A rapid technique for selection of mutants deficient in each of these enzymes was developed using sensitivity to infection by bacteriophage SP50 as an indication of the levels of enzyme. Mutants defective in BglI, BglII, and both BglI and BglII retained the wild-type modification phenotype. Genetic and biochemical studies have established that these enzymes are involved in restriction in vivo. Simplified purification procedures for BglI and BglII using these mutants are described.

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Year: 1978 PMID： 649568 PMCID： PMC222251 DOI： 10.1128/jb.134.1.338-344.1978

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

19 in total

1. Isolation and properties of a thermostable restriction endonuclease (ENDO R-Bst1503).

Authors: J F Catterall; N E Welker
Journal: J Bacteriol Date: 1977-02 Impact factor: 3.490

2. INFECTIOUS DNA FROM A NEWLY ISOLATED B. SUBTILIS PHAGE.

Authors: J FOELDES; T A TRAUTNER
Journal: Z Vererbungsl Date: 1964-04-10

3. Two restriction endonucleases from Bacillus globiggi.

Authors: V Pirrotta
Journal: Nucleic Acids Res Date: 1976-07 Impact factor: 16.971

4. R factor-controlled restriction and modification of deoxyribonucleic acid: restriction mutants.

Authors: R Yoshimori; D Roulland-Dussoix; H W Boyer
Journal: J Bacteriol Date: 1972-12 Impact factor: 3.490

5. Host specificity of DNA in Haemophilus influenzae: the two restriction and modification systems in strain Ra.

Authors: A Piekarowicz; S W Glover
Journal: Mol Gen Genet Date: 1972

6. Restriction and modification in B. subtilis. Biological aspects.

Authors: T A Trautner; B Pawlek; S Bron; C Anagnostopoulos
Journal: Mol Gen Genet Date: 1974

7. Genetic and transfection studies with B. subtilis phage SP50. 3. Biological effects of DNA cleavage and the physical basis of the map.

Authors: W R Havender; T A Trautner
Journal: Mol Gen Genet Date: 1972

8. Transformation of Bacillus subtilis and Escherichia coli by a hybrid plasmid pCD1.

Authors: C H Duncan; G A Wilson; F E Young
Journal: Gene Date: 1977-03 Impact factor: 3.688

9. Regulation of the bacterial cell wall: analysis of a mutant of Bacillus subtilis defective in biosynthesis of teichoic acid.

Authors: R J Boylan; N H Mendelson; D Brooks; F E Young
Journal: J Bacteriol Date: 1972-04 Impact factor: 3.490

10. Intergenotic transformation of the Bacillus subtilis genospecies.

Authors: G A Wilson; F E Young
Journal: J Bacteriol Date: 1972-09 Impact factor: 3.490

25 in total

1. The plastome of a brown alga,Dictyota dichotoma : I. Physical properties and the Bam HI/Sal I/Bgl II cleavage site map.

Authors: M Kuhsel; K V Kowallik
Journal: Plant Mol Biol Date: 1985-11 Impact factor: 4.076

2. Restriction enzymes and their isoschizomers.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1990-04-25 Impact factor: 16.971

3. Restriction enzymes and their isoschizomers.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1989 Impact factor: 16.971

4. A simple and rapid method for screening bacteria for type II restriction endonucleases: enzymes in Aphanothece halophytica.

Authors: P R Whitehead; N L Brown
Journal: Arch Microbiol Date: 1985-02 Impact factor: 2.552

5. Restriction and modification enzymes and their recognition sequences.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1985 Impact factor: 16.971

6. Synthesis of decadeoxyribonucleotides containing N6-methyladenine, N4-methylcytosine, and 5-methylcytosine: recognition and cleavage by restriction endonucleases (nucleosides and nucleotides part 74).

Authors: A Ono; T Ueda
Journal: Nucleic Acids Res Date: 1987-01-12 Impact factor: 16.971

7. RNA polymerase III transcriptional units are interspersed among human non-alpha-globin genes.

Authors: C Duncan; P A Biro; P V Choudary; J T Elder; R R Wang; B G Forget; J K de Riel; S M Weissman
Journal: Proc Natl Acad Sci U S A Date: 1979-10 Impact factor: 11.205

8. Restriction enzymes and their isoschizomers.

Authors: R J Roberts
Journal: Nucleic Acids Res Date: 1988 Impact factor: 16.971

9. Analysis of the recognition mechanism involved in the EcoRV catalyzed cleavage of DNA using modified oligodeoxynucleotides.

Authors: A Fliess; H Wolfes; F Seela; A Pingoud
Journal: Nucleic Acids Res Date: 1988-12-23 Impact factor: 16.971

10. Unusually infrequent cleavage with several endonucleases and physical map construction of Bacillus subtilis bacteriophage phi 1 DNA.

Authors: F Kawamura; T Mizukami; H Shimotsu; H Anzai; H Takahashi; H Saito
Journal: J Virol Date: 1981-03 Impact factor: 5.103