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Literature DB >> 3023973

High-efficiency transformation of Saccharomyces cerevisiae cells by bacterial minicell protoplast fusion.

Abstract

After a new transformation procedure, 10% of Saccharomyces cerevisiae cells were found to contain transforming DNA sequences. We used direct transfer of plasmid molecules by fusing bacterial minicell protoplasts to yeast protoplasts. Since the procedure significantly reduces the toxic effect of procaryotic protoplasm on the eucaryotic organism, it might be generally applicable in other systems in which transformation is inefficient or impossible.

Entities: Disease Species

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Year: 1986 PMID： 3023973 PMCID： PMC367070 DOI： 10.1128/mcb.6.9.3295-3297.1986

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

8 in total

1. Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.

Authors: J R Broach; J N Strathern; J B Hicks
Journal: Gene Date: 1979-12 Impact factor: 3.688

2. A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors: F L Graham; A J van der Eb
Journal: Virology Date: 1973-04 Impact factor: 3.616

3. Methods for micromanipulation of human somatic cells in culture.

Authors: E G Diacumakos
Journal: Methods Cell Biol Date: 1973 Impact factor: 1.441

4. Use of minicells for bacteriophage-directed polypeptide synthesis.

Authors: J Reeve
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

5. Transformation of yeast by a replicating hybrid plasmid.

Authors: J D Beggs
Journal: Nature Date: 1978-09-14 Impact factor: 49.962

6. Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.

Authors: M J Dobson; M F Tuite; N A Roberts; A J Kingsman; S M Kingsman; R E Perkins; S C Conroy; L A Fothergill
Journal: Nucleic Acids Res Date: 1982-04-24 Impact factor: 16.971

7. Direct transfer of cloned genes from bacteria to mammalian cells.

Authors: W Schaffner
Journal: Proc Natl Acad Sci U S A Date: 1980-04 Impact factor: 11.205

8. The herpes simplex virus thymidine kinase gene is not transcribed in Saccharomyces cerevisiae.

Authors: G B Kiss; R E Pearlman; K V Cornish; J D Friesen; V L Chan
Journal: J Bacteriol Date: 1982-02 Impact factor: 3.490

8 in total

7 in total

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2. Transferring whole genomes from bacteria to yeast spheroplasts using entire bacterial cells to reduce DNA shearing.

Authors: Bogumil J Karas; Jelena Jablanovic; Edward Irvine; Lijie Sun; Li Ma; Philip D Weyman; Daniel G Gibson; John I Glass; J Craig Venter; Clyde A Hutchison; Hamilton O Smith; Yo Suzuki
Journal: Nat Protoc Date: 2014-03-06 Impact factor: 13.491

3. Direct transfer of whole genomes from bacteria to yeast.

Authors: Bogumil J Karas; Jelena Jablanovic; Lijie Sun; Li Ma; Gregory M Goldgof; Jason Stam; Adi Ramon; Micah J Manary; Elizabeth A Winzeler; J Craig Venter; Philip D Weyman; Daniel G Gibson; John I Glass; Clyde A Hutchison; Hamilton O Smith; Yo Suzuki
Journal: Nat Methods Date: 2013-03-31 Impact factor: 28.547