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Literature DB >> 3282693

A colony procedure for transformation of Saccharomyces cerevisiae.

Abstract

A rapid and simple yeast transformation procedure has been developed using colonies on agar plates. Saccharomyces cerevisiae SHY3 cells were picked up from colonies on YPD plates grown freshly or stored at 4 degrees C and incubated with M13RK9-T DNA at 30 degrees C for 1-2 h in a solution of Li+, Ca2+, Mg2+, triacetin and polyethylene glycol. About 3,500 transformants were obtained per microgram of double stranded M13RK9-T DNA. Unlike the existing spheroplast techniques, single stranded M13RK9-T DNA transformed intact cells below one-hundredth frequency of the duplex form.

Entities: Chemical Species

Mesh：

Year: 1988 PMID： 3282693 DOI： 10.1007/bf00365751

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

17 in total

1. Transformation of Bacillus subtilis by single-stranded plasmid DNA.

Authors: C F Rudolph; B J Schmidt; C W Saunders
Journal: J Bacteriol Date: 1986-03 Impact factor: 3.490

2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors: H C Birnboim; J Doly
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

3. Transformation of protoplasted yeast cells is directly associated with cell fusion.

Authors: S Harashima; A Takagi; Y Oshima
Journal: Mol Cell Biol Date: 1984-04 Impact factor: 4.272

4. New M13 vectors for cloning.

Authors: J Messing
Journal: Methods Enzymol Date: 1983 Impact factor: 1.600

5. Factors enhancing genetic transformation of intact yeast cells modify cell wall porosity.

Authors: B Brzobohatý; L Kovác
Journal: J Gen Microbiol Date: 1986-11

6. One strand of ars189 from the maxicircle of Crithidia fasciculata transforms Saccharomyces cerevisiae more efficiently than its complementary strand as a single stranded DNA.

Authors: R Kim; D S Ray
Journal: Gene Date: 1985 Impact factor: 3.688

5. The Arabidopsis thaliana RER1 gene family: its potential role in the endoplasmic reticulum localization of membrane proteins.

Authors: K Sato; T Ueda; A Nakano
Journal: Plant Mol Biol Date: 1999-12 Impact factor: 4.076

6. Inhibition of endoplasmic reticulum (ER)-to-Golgi transport induces relocalization of binding protein (BiP) within the ER to form the BiP bodies.

Authors: S Nishikawa; A Hirata; A Nakano
Journal: Mol Biol Cell Date: 1994-10 Impact factor: 4.138

7. Parameters affecting lithium acetate-mediated transformation of Saccharomyces cerevisiae and development of a rapid and simplified procedure.

Authors: R Soni; J P Carmichael; J A Murray
Journal: Curr Genet Date: 1993-11 Impact factor: 3.886

8. Enhancement of plasmid DNA transformation efficiencies in early stationary-phase yeast cell cultures.

Authors: Jennifer DeMars Tripp; Jennifer L Lilley; Whitney N Wood; L Kevin Lewis
Journal: Yeast Date: 2013-04-12 Impact factor: 3.239

9. Ethanol improves the transformation efficiency of intact yeast cells.

Authors: V Lauermann
Journal: Curr Genet Date: 1991-07 Impact factor: 3.886

10. Genetic evidence for the roles of the bud-site-selection genes BUD5 and BUD2 in control of the Rsr1p (Bud1p) GTPase in yeast.

Authors: A Bender
Journal: Proc Natl Acad Sci U S A Date: 1993-11-01 Impact factor: 11.205

A colony procedure for transformation of Saccharomyces cerevisiae.

1. Transformation of Bacillus subtilis by single-stranded plasmid DNA.

2. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

3. Transformation of protoplasted yeast cells is directly associated with cell fusion.

4. New M13 vectors for cloning.

5. Factors enhancing genetic transformation of intact yeast cells modify cell wall porosity.

6. One strand of ars189 from the maxicircle of Crithidia fasciculata transforms Saccharomyces cerevisiae more efficiently than its complementary strand as a single stranded DNA.

7. Transformation of yeast by a replicating hybrid plasmid.

8. Transformation of yeast.

9. A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast.

10. Studies on transformation of Escherichia coli with plasmids.

1. Rer1p as common machinery for the endoplasmic reticulum localization of membrane proteins.

2. APT1, but not APT2, codes for a functional adenine phosphoribosyltransferase in Saccharomyces cerevisiae.

3. Site-directed mutagenesis of the Saccharomyces cerevisiae CDC25 gene: effects on mitotic growth and cAMP signalling.