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Literature DB >> 6276360

The herpes simplex virus thymidine kinase gene is not transcribed in Saccharomyces cerevisiae.

G B Kiss, R E Pearlman, K V Cornish, J D Friesen, V L Chan.

Abstract

The herpes simplex virus thymidine kinase gene has been cloned into a chimeric yeast plasmid cloning vehicle and transformed into appropriate yeast strains. Plasmids carrying the herpes simplex virus thymidine kinase gene can be propagated as autonomously replicating plasmids, but no RNA specific to the thymidine kinase coding sequence was detected.

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Year: 1982 PMID： 6276360 PMCID： PMC216540 DOI： 10.1128/jb.149.2.542-547.1982

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

25 in total

1. Isolation of yeast DNA.

Authors: D R Cryer; R Eccleshall; J Marmur
Journal: Methods Cell Biol Date: 1975 Impact factor: 1.441

2. Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA.

Authors: S Bacchetti; F L Graham
Journal: Proc Natl Acad Sci U S A Date: 1977-04 Impact factor: 11.205

3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

4. Cloning of herpes simplex type 1 DNA fragments in a bacteriophage lambda vector.

Authors: L W Enquist; M J Madden; P Schiop-Stanley; G F Vande Woude
Journal: Science Date: 1979-02-09 Impact factor: 47.728

5. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.

Authors: M Wigler; A Pellicer; S Silverstein; R Axel
Journal: Cell Date: 1978-07 Impact factor: 41.582

6. The synthesis of eucaryotic ribosomal proteins in vitro.

Authors: J R Warner; C Gorenstein
Journal: Cell Date: 1977-05 Impact factor: 41.582

7. A virus-specific thymidine kinase in BHK-21 cells infected with herpes simplex virus.

Authors: H G Klemperer; G R Haynes; W I Shedden; D H Watson
Journal: Virology Date: 1967-01 Impact factor: 3.616

8. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.

Authors: D B Clewell; D R Helinski
Journal: Biochemistry Date: 1970-10-27 Impact factor: 3.162

9. Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid.

Authors: A R Grivell; J F Jackson
Journal: J Gen Microbiol Date: 1968-12

The herpes simplex virus thymidine kinase gene is not transcribed in Saccharomyces cerevisiae.

1. Isolation of yeast DNA.

2. Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA.

3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

4. Cloning of herpes simplex type 1 DNA fragments in a bacteriophage lambda vector.

5. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.

6. The synthesis of eucaryotic ribosomal proteins in vitro.

7. A virus-specific thymidine kinase in BHK-21 cells infected with herpes simplex virus.

8. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.

9. Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid.

10. Transformation of yeast.

1. Development of a eukaryotic cloning system in Podospora anserina : I. Long-lived mutants as potential recipients.

2. Cloning vectors of mitochondrial origin for eukaryotes: A new concept in genetic engineering.

3. High-efficiency transformation of Saccharomyces cerevisiae cells by bacterial minicell protoplast fusion.

4. Expression of the major heat shock gene of Drosophila melanogaster in Saccharomyces cerevisiae.

5. Yeast/herpes simplex virus thymidine kinase gene fusions yield fusion proteins with thymidine kinase activity.

6. Efficient transformation of Dictyostelium discoideum amoebae.

7. Regulated high efficiency expression of human interferon-alpha in Saccharomyces cerevisiae.