Literature DB >> 395029

Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.

J R Broach, J N Strathern, J B Hicks.   

Abstract

We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2 mu circule. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.

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Year:  1979        PMID： 395029     DOI： 10.1016/0378-1119(79)90012-x

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  481 in total

1.  Evidence for a protein mutator in yeast: role of the Hsp70-related chaperone ssb in formation, stability, and toxicity of the [PSI] prion.

Authors:  Y O Chernoff; G P Newnam; J Kumar; K Allen; A D Zink
Journal:  Mol Cell Biol       Date:  1999-12       Impact factor: 4.272

2.  Analysis of yeast retrotransposon Ty insertions at the CAN1 locus.

Authors:  C M Wilke; S H Heidler; N Brown; S W Liebman
Journal:  Genetics       Date:  1989-12       Impact factor: 4.562

3.  Saccharomyces cerevisiae coordinates accumulation of yeast ribosomal proteins by modulating mRNA splicing, translational initiation, and protein turnover.

Authors:  J R Warner; G Mitra; W F Schwindinger; M Studeny; H M Fried
Journal:  Mol Cell Biol       Date:  1985-06       Impact factor: 4.272

4.  Isolation of the catalase A gene of Saccharomyces cerevisiae by complementation of the cta1 mutation.

Authors:  G Cohen; F Fessl; A Traczyk; J Rytka; H Ruis
Journal:  Mol Gen Genet       Date:  1985

5.  Cauliflower mosaic virus promoters direct efficient expression of a bacterial G418 resistance gene in Schizosaccharomyces pombe.

Authors:  H Gmünder; J Kohli
Journal:  Mol Gen Genet       Date:  1989-12

6.  Identification and cloning of a yeast nuclear gene (CBP1) involved in expression of mitochondrial cytochrome b.

Authors:  C L Dieckmann; L K Pape; A Tzagoloff
Journal:  Proc Natl Acad Sci U S A       Date:  1982-03       Impact factor: 11.205

7.  Molecular genetics of cryptopleurine resistance in Saccharomyces cerevisiae: expression of a ribosomal protein gene family.

Authors:  A G Paulovich; J R Thompson; J C Larkin; Z Li; J L Woolford
Journal:  Genetics       Date:  1993-11       Impact factor: 4.562

8.  Cloning and manipulation of the Schizosaccharomyces pombe his7+ gene as a new selectable marker for molecular genetic studies.

Authors:  E Apolinario; M Nocero; M Jin; C S Hoffman
Journal:  Curr Genet       Date:  1993-12       Impact factor: 3.886

9.  Oxygen regulation of anaerobic and aerobic genes mediated by a common factor in yeast.

Authors:  C V Lowry; R S Zitomer
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

10.  Temperature-sensitive lethal mutations on yeast chromosome I appear to define only a small number of genes.

Authors:  D B Kaback; P W Oeller; H Yde Steensma; J Hirschman; D Ruezinsky; K G Coleman; J R Pringle
Journal:  Genetics       Date:  1984-09       Impact factor: 4.562
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