Cheryl Jennings1, Brian Harty2, Salvatore R Scianna3, Suzanne Granger2, Amy Couzens4, Daniel Zaccaro4, James W Bremer3. 1. Rush Medical College, Department of Microbial Pathogens and Immunity, Chicago, IL, USA. Electronic address: cheryl_jennings@rush.edu. 2. New England Research Institute, Boston, MA, USA. 3. Rush Medical College, Department of Microbial Pathogens and Immunity, Chicago, IL, USA. 4. RTI International, Research Triangle Park, NC, USA.
Abstract
BACKGROUND: Commercially-available kits for HIV-1 detection include instructions for detecting HIV-1 in plasma and DBS, but don't support other specimen types. OBJECTIVES: Show quantitative stability of HIV-1 total nucleic acid (TNA) in blood and improved HIV-1 detection in alternative specimen types. STUDY DESIGN: Whole blood and DBS specimens, tested as part of an external quality assurance program for qualitative HIV-1 detection, were used to evaluated error rates (false negative [FN], false positive [FP] and indeterminant [IND] results) across assays (internally developed [IH], Roche Amplicor [RA], and Roche TaqMan Qual [TQ]) and specimen types (frozen whole blood [BLD], DBS and cell pellets [PEL]). A modified Roche TaqMan HIV-1 assay was used to quantify HIV-1 TNA. RESULTS: Significantly higher error rates were noted in DBS across all of the assays (4% vs. 0% for DBS and PEL, IH, p = 0.005; 4% vs. 0.1% for DBS and PEL, RA, p < 0.001; 10% vs. 1% for DBS and PEL or BLD, TQ, p < 0.001). HIV TNA concentration is stable in BLD (day 1 vs. day 10, p = 0.39) and higher than DBS (p < 0.001). CONCLUSIONS: Transporting refrigerated whole blood for centralized processing into alternative specimen types will improve the sensitivitiy of HIV-1 detection in samples with low virus loads.
BACKGROUND: Commercially-available kits for HIV-1 detection include instructions for detecting HIV-1 in plasma and DBS, but don't support other specimen types. OBJECTIVES: Show quantitative stability of HIV-1 total nucleic acid (TNA) in blood and improved HIV-1 detection in alternative specimen types. STUDY DESIGN: Whole blood and DBS specimens, tested as part of an external quality assurance program for qualitative HIV-1 detection, were used to evaluated error rates (false negative [FN], false positive [FP] and indeterminant [IND] results) across assays (internally developed [IH], Roche Amplicor [RA], and Roche TaqMan Qual [TQ]) and specimen types (frozen whole blood [BLD], DBS and cell pellets [PEL]). A modified Roche TaqMan HIV-1 assay was used to quantify HIV-1 TNA. RESULTS: Significantly higher error rates were noted in DBS across all of the assays (4% vs. 0% for DBS and PEL, IH, p = 0.005; 4% vs. 0.1% for DBS and PEL, RA, p < 0.001; 10% vs. 1% for DBS and PEL or BLD, TQ, p < 0.001). HIV TNA concentration is stable in BLD (day 1 vs. day 10, p = 0.39) and higher than DBS (p < 0.001). CONCLUSIONS: Transporting refrigerated whole blood for centralized processing into alternative specimen types will improve the sensitivitiy of HIV-1 detection in samples with low virus loads.
Authors: K Kébé; O Ndiaye; H Diop Ndiaye; P Mbakob Mengue; P M M Guindo; S Diallo; N Léye; S B Gueye; A Gaye Diallo; C Touré Kane; S Mboup Journal: J Clin Microbiol Date: 2011-05-04 Impact factor: 5.948
Authors: Caroline Mitchell; Cheryl Jennings; Donald Brambilla; Grace Aldrovandi; Angela Martin Amedee; Ingrid Beck; James W Bremer; Robert Coombs; Don Decker; Susan Fiscus; Joseph Fitzgibbon; Katherine Luzuriaga; John Moye; Paul Palumbo; Patricia Reichelderfer; Mohan Somasundaran; Wendy Stevens; Lisa Frenkel Journal: J Clin Microbiol Date: 2008-07-09 Impact factor: 5.948
Authors: A Fischer; C Lejczak; C Lambert; J Servais; N Makombe; J Rusine; T Staub; R Hemmer; F Schneider; J C Schmit; V Arendt Journal: J Clin Microbiol Date: 2004-01 Impact factor: 5.948
Authors: Cheryl Jennings; Neil T Parkin; Daniel J Zaccaro; Rupert Capina; Paul Sandstrom; Hezhao Ji; Donald J Brambilla; James W Bremer Journal: Viruses Date: 2020-12-17 Impact factor: 5.048
Authors: Nishi Prabdial-Sing; Lucinda Gaelejwe; Lillian Makhathini; Jayendrie Thaver; Morubula Jack Manamela; Susan Malfeld; C Wendy Spearman; Mark Sonderup; Andrew Scheibe; Katherine Young; Harry Hausler; Adrian J Puren Journal: Health Sci Rep Date: 2021-02-11