Literature DB >> 21543563

RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA test v1.5) for early diagnosis of HIV-1 infection in infants in Senegal.

K Kébé1, O Ndiaye, H Diop Ndiaye, P Mbakob Mengue, P M M Guindo, S Diallo, N Léye, S B Gueye, A Gaye Diallo, C Touré Kane, S Mboup.   

Abstract

The objective of this study was to compare the performance of the NucliSENS EasyQ HIV-1 v1.2 platform (bioMérieux, France) to the Amplicor HIV-1 DNA test v1.5 (Roche Molecular Systems, Switzerland) in detecting HIV-1 infection in infants using venipuncture-derived whole blood in tubes and dried blood spots. A total of 149 dried blood spots and 43 EDTA-anticoagulated peripheral blood samples were collected throughout Dakar and other areas in Senegal from infants and children aged 3 weeks to 24 months who were born to HIV-1-infected mothers. Samples were tested using the NucliSENS and Amplicor technologies. The NucliSENS and Amplicor results were 100% concordant using either EDTA-anticoagulated peripheral blood or dried blood spots. Compared to Amplicor, the sensitivity and specificity of the NucliSENS test were 100%. The NucliSENS EasyQ HIV-1 RNA assay performed as well as the Amplicor HIV-1 DNA test in detecting HIV-1 infection in infants. In addition, this platform can give an indication of the viral load baseline. The NucliSENS EasyQ platform is a good alternative for early infant diagnosis of HIV-1 infection.

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Year:  2011        PMID： 21543563      PMCID： PMC3147881          DOI： 10.1128/JCM.02402-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  31 in total

1.  Quantitation of HIV-1 RNA in dried blood and plasma spots.

Authors:  S Reigadas; M H Schrive; V Aurillac-Lavignolle; H J Fleury
Journal:  J Virol Methods       Date:  2009-06-11       Impact factor: 2.014

2.  Natural history of human immunodeficiency virus type 1 infection in children: a five-year prospective study in Rwanda. Mother-to-Child HIV-1 Transmission Study Group.

Authors:  R Spira; P Lepage; P Msellati; P Van De Perre; V Leroy; A Simonon; E Karita; F Dabis
Journal:  Pediatrics       Date:  1999-11       Impact factor: 7.124

3.  Early diagnosis of human immunodeficiency virus-1 infection in infants with the NucliSens EasyQ assay on dried blood spots.

Authors:  R R Lilian; K Bhowan; G G Sherman
Journal:  J Clin Virol       Date:  2010-03-07       Impact factor: 3.168

4.  Early infant diagnosis of HIV infection in southeastern Nigeria: prevalence of HIV infection among HIV-exposed babies.

Authors:  E F Ugochukwu; S O Kanu
Journal:  West Afr J Med       Date:  2010 Jan-Feb

5.  Quantitative detection of plasma human immunodeficiency virus type 2 subtype A RNA by the Nuclisens EasyQ Assay (version 1.1).

Authors:  Berta Rodés; Julie Sheldon; Carlos Toro; Laureano Cuevas; Esperanza Pérez-Pastrana; Inmaculada Herrera; Vincent Soriano
Journal:  J Clin Microbiol       Date:  2006-11-08       Impact factor: 5.948

6.  Dried blood spots for the diagnosis and quantitation of HIV-1: stability studies and evaluation of sensitivity and specificity for the diagnosis of infant HIV-1 infection in Thailand.

Authors:  W Leelawiwat; N L Young; T Chaowanachan; C Y Ou; M Culnane; N Vanprapa; N Waranawat; P Wasinrapee; P A Mock; J Tappero; J M McNicholl
Journal:  J Virol Methods       Date:  2008-11-18       Impact factor: 2.014

7.  Early HIV-1 diagnosis using in-house real-time PCR amplification on dried blood spots for infants in remote and resource-limited settings.

Authors:  Nicole Ngo-Giang-Huong; Woottichai Khamduang; Baptiste Leurent; Intira Collins; Issaren Nantasen; Pranee Leechanachai; Wasna Sirirungsi; Aram Limtrakul; Tasana Leusaree; Anne Marie Comeau; Marc Lallemant; Gonzague Jourdain
Journal:  J Acquir Immune Defic Syndr       Date:  2008-12-15       Impact factor: 3.731

8.  Evaluation of a dried blood spot HIV-1 RNA program for early infant diagnosis and viral load monitoring at rural and remote healthcare facilities.

Authors:  Sarah M Lofgren; Anne B Morrissey; Caroline C Chevallier; Anangisye I Malabeja; Sally Edmonds; Ben Amos; David J Sifuna; Lorenz von Seidlein; Werner Schimana; Wendy S Stevens; John A Bartlett; John A Crump
Journal:  AIDS       Date:  2009-11-27       Impact factor: 4.177

9.  Early antiretroviral therapy and mortality among HIV-infected infants.

Authors:  Avy Violari; Mark F Cotton; Diana M Gibb; Abdel G Babiker; Jan Steyn; Shabir A Madhi; Patrick Jean-Philippe; James A McIntyre
Journal:  N Engl J Med       Date:  2008-11-20       Impact factor: 91.245

10.  Low-cost HIV-1 diagnosis and quantification in dried blood spots by real time PCR.

Authors:  Nishaki Mehta; Sonia Trzmielina; Bareng A S Nonyane; Melissa N Eliot; Rongheng Lin; Andrea S Foulkes; Kristina McNeal; Arthur Ammann; Vindu Eulalievyolo; John L Sullivan; Katherine Luzuriaga; Mohan Somasundaran
Journal:  PLoS One       Date:  2009-06-05       Impact factor: 3.240
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  8 in total

1.  The stability of HIV-1 nucleic acid in whole blood and improved detection of HIV-1 in alternative specimen types when compared to Dried Blood Spot (DBS) specimens.

Authors:  Cheryl Jennings; Brian Harty; Salvatore R Scianna; Suzanne Granger; Amy Couzens; Daniel Zaccaro; James W Bremer
Journal:  J Virol Methods       Date:  2018-08-17       Impact factor: 2.014

2.  Single genome amplification of proviral HIV-1 DNA from dried blood spot specimens collected during early infant screening programs in Lusaka, Zambia.

Authors:  Lillian Seu; Innocent Mwape; M Bradford Guffey
Journal:  J Virol Methods       Date:  2014-03-22       Impact factor: 2.014

3.  Enhancing Maternal and Child Health using a Combined Mother & Child Health Booklet in Kenya.

Authors:  Mildred A Mudany; Martin Sirengo; George W Rutherford; Mary Mwangi; Lucy W Nganga; Anthony Gichangi
Journal:  J Trop Pediatr       Date:  2015-09-04       Impact factor: 1.165

4.  Rapid detection of HIV-1 proviral DNA for early infant diagnosis using recombinase polymerase amplification.

Authors:  David S Boyle; Dara A Lehman; Lorraine Lillis; Dylan Peterson; Mitra Singhal; Niall Armes; Mathew Parker; Olaf Piepenburg; Julie Overbaugh
Journal:  MBio       Date:  2013-04-02       Impact factor: 7.867

Review 5.  Systematic review of the use of dried blood spots for monitoring HIV viral load and for early infant diagnosis.

Authors:  Pieter W Smit; Kimberly A Sollis; Susan Fiscus; Nathan Ford; Marco Vitoria; Shaffiq Essajee; David Barnett; Ben Cheng; Suzanne M Crowe; Thomas Denny; Alan Landay; Wendy Stevens; Vincent Habiyambere; Joseph H Perriens; Rosanna W Peeling
Journal:  PLoS One       Date:  2014-03-06       Impact factor: 3.240

Review 6.  Diagnostic devices for isothermal nucleic acid amplification.

Authors:  Chia-Chen Chang; Chien-Cheng Chen; Shih-Chung Wei; Hui-Hsin Lu; Yang-Hung Liang; Chii-Wann Lin
Journal:  Sensors (Basel)       Date:  2012-06-14       Impact factor: 3.576

Review 7.  An overview of the clinical use of filter paper in the diagnosis of tropical diseases.

Authors:  Pieter W Smit; Ivo Elliott; Rosanna W Peeling; David Mabey; Paul N Newton
Journal:  Am J Trop Med Hyg       Date:  2013-12-23       Impact factor: 2.345

Review 8.  Point-of-care tests detecting HIV nucleic acids for diagnosis of HIV-1 or HIV-2 infection in infants and children aged 18 months or less.

Authors:  Eleanor A Ochodo; Fatuma Guleid; Jonathan J Deeks; Sue Mallett
Journal:  Cochrane Database Syst Rev       Date:  2021-08-12
  8 in total

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