Literature DB >> 18199786

Comparison of results obtained with Amplicor HIV-1 DNA PCR test version 1.5 using 100 versus 500 microliters of whole blood.

Estelle Piwowar-Manning1, Lebah Lugalia, Bosco Kafufu, J Brooks Jackson.   

Abstract

The Amplicor HIV-1 DNA PCR assay (Roche Diagnostics, Branchburg, NJ) requires 500 microl of whole blood for a diagnosis of human immunodeficiency virus type 1 (HIV-1) infection, and this amount is often difficult to obtain from infants. A comparison was performed using 100 and 500 microl of whole blood from infants less than 18 months of age. The concordance rate for HIV DNA PCR-negative and -positive samples was 100% for the two different volumes.

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Year:  2008        PMID： 18199786      PMCID： PMC2268375          DOI： 10.1128/JCM.02259-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  3 in total

1.  Detection of HIV-1 proviral DNA with the AMPLICOR HIV-1 DNA Test, version 1.5, following sample processing by the MagNA Pure LC instrument.

Authors:  Jeffrey J Germer; Tara M Gerads; Jayawant N Mandrekar; P Shawn Mitchell; Joseph D C Yao
Journal:  J Clin Virol       Date:  2006-09-12       Impact factor: 3.168

2.  Evaluation of dried whole blood spots obtained by heel or finger stick as an alternative to venous blood for diagnosis of human immunodeficiency virus type 1 infection in vertically exposed infants in the routine diagnostic laboratory.

Authors:  Janet C Patton; Eveline Akkers; Ashraf H Coovadia; Tammy M Meyers; Wendy S Stevens; Gayle G Sherman
Journal:  Clin Vaccine Immunol       Date:  2006-12-13

Review 3.  Infant human immunodeficiency virus diagnosis in resource-limited settings: issues, technologies, and country experiences.

Authors:  Tracy L Creek; Gayle G Sherman; John Nkengasong; Lydia Lu; Thomas Finkbeiner; Mary Glenn Fowler; Emilia Rivadeneira; Nathan Shaffer
Journal:  Am J Obstet Gynecol       Date:  2007-09       Impact factor: 8.661
  3 in total
  4 in total

1.  The stability of HIV-1 nucleic acid in whole blood and improved detection of HIV-1 in alternative specimen types when compared to Dried Blood Spot (DBS) specimens.

Authors:  Cheryl Jennings; Brian Harty; Salvatore R Scianna; Suzanne Granger; Amy Couzens; Daniel Zaccaro; James W Bremer
Journal:  J Virol Methods       Date:  2018-08-17       Impact factor: 2.014

2.  RNA versus DNA (NucliSENS EasyQ HIV-1 v1.2 versus Amplicor HIV-1 DNA test v1.5) for early diagnosis of HIV-1 infection in infants in Senegal.

Authors:  K Kébé; O Ndiaye; H Diop Ndiaye; P Mbakob Mengue; P M M Guindo; S Diallo; N Léye; S B Gueye; A Gaye Diallo; C Touré Kane; S Mboup
Journal:  J Clin Microbiol       Date:  2011-05-04       Impact factor: 5.948

3.  Potential for false positive HIV test results with the serial rapid HIV testing algorithm.

Authors:  Steven Baveewo; Moses R Kamya; Harriet Mayanja-Kizza; Robin Fatch; David R Bangsberg; Thomas Coates; Judith A Hahn; Rhoda K Wanyenze
Journal:  BMC Res Notes       Date:  2012-03-19

4.  The case for addressing primary resistance mutations to non-nucleoside reverse transcriptase inhibitors to treat children born from mothers living with HIV in sub-Saharan Africa.

Authors:  Khady Kébé; Laurent Bélec; Halimatou Diop Ndiaye; Sokhna Bousso Gueye; Abou Abdallah Malick Diouara; Safiétou Ngom; Ndéye Rama Diagne Gueye; Ngagne Mbaye; Haby Signaté Sy; Souleymane Mboup; Coumba Touré Kane
Journal:  J Int AIDS Soc       Date:  2014-01-16       Impact factor: 5.396
  4 in total

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