New Cembranoids and a Biscembranoid Peroxide from the Soft Coral Sarcophyton cherbonnieri.

Six new cembranoids, cherbonolides A-E (1⁻5) and bischerbolide peroxide (6), along with one known cembranoid, isosarcophine (7), were isolated from the Formosan soft coral Sarcophyton cherbonnieri. The structures of these compounds were elucidated by detailed spectroscopic analysis and chemical methods. Compound 6 was discovered to be the first example of a molecular skeleton formed from two cembranoids connected by a peroxide group. Compounds 1⁻7 were shown to have the ability of inhibiting the production of superoxide anions and elastase release in N-formyl-methionyl-leucyl-phenyl-alanine/cytochalasin B (fMLF/CB)-induced human neutrophils.

1. Introduction

Many cembrane-based natural products have been shown to exhibit significant activities such as cytotoxicity [1,2,3,4,5,6,7,8,9,10,11,12,13,14] and anti-inflammatory activity [9,11,13,14,15,16,17,18]. From the experience of searching bioactive metabolites from soft corals, series of cembranoids have been unveiled from octocorals (Alcyonaceae) belonging to the genera Sarcophyton, [1,2,3,4,5,6,7,8,16], Sinularia [9,10,11,12,17,18] and Lobophyton [13,14,15]. Also, previous studies showed that two cembranoid units could form biscembranoid-type compounds by Diels-Alder reaction [19,20,21], radical dimerization [22,23], or connection with a sulfur atom [18], making the chemistry of cembranes more complex and interesting than monomeric form.

Our current chemical investigation on Sarcophyton cherbonnieri led to the discovery of six new cembranoids, cherbonolides A−E (1–5) and bischerbolide peroxide (6), and one known compound, isosarcophine (7) [24]. The structures of 1–7 (Figure 1) were elucidated by extensive spectroscopic analysis, including detailed 2D nuclear magnetic resonance (NMR) experiments and chemical methods. Compounds 2, 5, and 6 were characterized as cembranoids bearing an allylic peroxyl group as those previously discovered marine cembranoidal peroxides [25,26,27,28,29]. Furthermore, compound 6 is the first example of a biscembranoid with two cembranoidal units interconnected by a peroxyl group. The absolute configurations of 1 and 3 were further established using a modified Mosher’s reaction. Also, evaluation of the in vitro anti-inflammatory activities through the inhibition of superoxide anion (O2−•) generation and elastase release in N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLF/CB)-induced human neutrophils was carried out.

Figure 1

Cembranoid isolated from Sarcophyton cherbonnieri.

2. Results and Discussion

The soft coral S. cherbonnieri (1.2 kg, wet weight) was collected using SCUBA diving from Jihui Port of Taitung, Taiwan, in March 2013, and stored in a freezer before extraction. The freeze-dried organisms (207 g) were sliced into small pieces, followed by exhaustive extraction with ethyl acetate (EtOAc). The EtOAc extract was dried with anhydrous sodium sulfate (Na2SO4). After removal of EtOAc under reduced pressure, the residue yielded was separated by silica gel column chromatography and the resolved fractions were further purified by reverse-phase C18 high-performance liquid chromatography (HPLC) to afford compounds 1–7 (Figure 1), the structures of which were elucidated on the basis of spectroscopic analyses (Supplementary Materials, Figures S1–S46).

Cherbonolide A (1) was isolated as a colorless oil. The molecular formula C20H28O4 of 1 was determined by the high-resolution electrospray ionization mass spectrometry (HRESIMS) (m/z calcd 355.1880; found 355.1879, [M + Na]+), which required seven degrees of unsaturation. The IR spectrum of 1 showed the presence of a hydroxyl group (νmax 3457 cm−1) and a lactonic carbonyl group (νmax 1746 cm−1). The presence of 20 carbons in the structure of 1, including four methyls, five sp methylenes, three sp oxygenated methines, two sp methines, one sp and five sp nonprotoned carbon atoms were assigned with the aid of distortionless enhancement by polarization transfer (DEPT) spectra. The NMR peaks resonating at δC 174.4 (C), 160.7 (C), 123.8 (C), 77.8 (CH) and 8.8 (CH3), and δH 5.44 (1H, dd, J = 10.0, 1.6 Hz) and δH 1.86 (3H, s), are characteristic signals of an α-methyl-α,β-unsaturated-γ-lactone ring by comparison of the NMR data of the γ-lactone ring of known compound isosarcophine (7). Signals at δC 60.8 (C), 61.4 (CH) and δH 2.42 (1H, dd, J = 10.8, 2.8 Hz) showed the presence of a trisubstituted epoxide. Two trisubstituted double bonds could also be identified by NMR signals resonating at δC 122.2 (CH), δC 141.6 (C) and δH 4.90 (1H, d, J = 10.0 Hz), and at δC 128.1 (CH), δC 139.8 (C) and δH 5.20 (1H, d, J = 10.4 Hz), respectively. The correlations identified from the 1H-1H correlation spectroscopy (1H-1H COSY) spectrum revealed four separate proton sequences, as shown in Figure 2, which were assembled by heteronuclear multiple bond correlation (HMBC) correlations (Figure 2). Key HMBC correlations of H-2 (δH 5.44, 1H, dd, J = 10.0, 1.6 Hz) to C-1; H2-14 (δH 2.01, m)/C-1; H3-17 (δH 1.86, s) to C-1, C-15 and C-16; H3-18 (δH 1.70, s) to C-3, C-4 and C-5; H3-19 (δH 1.86, s) to C-7, C-8 and C-9; and H3-20 (δH 1.33, s) to C-11, C-12 and C-13, established the connection of the four proton sequences, and thus constructed the 14-membered ring carbon skeleton, which also indicated the presence of a hydroxyl at C-6. Thus, the planar structure of 1 was established.

Figure 2

Selected 1H-1H COSY and HMBC correlations of 1, 2 and 3−5.

Further, careful analysis of nuclear Overhauser enhancement (NOE) correlations was applied to establish the relative stereochemistry of 1, as shown in Figure 3. The NOE spectrum revealed that H-2 (δH 5.44, dd, J = 10.0, 1.6 Hz) showed NOE correction with H3-18 (δH 1.70, s); therefore, assuming a β-orientation of H-2, H3-18 should be β-oriented, too. Moreover, H3-18 exhibited NOE correlation with H-6 (δH 4.70, ddd, J = 10.4, 10.4, 5.2 Hz), revealing the β-orientation of H-6 and the R* configuration of C-6. One methylene proton at C-13 exhibited NOE correlation with H-2 and was characterized as H-13β (δH 1.06, m), while the other proton was assigned as H-13α (δH 2.03, m). NOE correlations of H-13β with H-11 (δH 2.42, dd, J = 10.8, 2.8 Hz) and H-13α with H3-20 (δH 1.33, s) reflected the β-orientation of H-11 and the α-orientation of H3-20, and hence the R* configurations of both C-11 and C-12. The E geometries of the trisubstituted C-3/C-4 and C-7/C-8 double bonds were also assigned from the NOE correlations of H3-18 (δH 1.70, s) with H-2, but not with H-3 (δH 4.90, d, J = 10.0 Hz), as well as H3-19 (δH 1.86, s) with H-6, but not with H-7 (δH 5.20, d, J = 10.4 Hz), and were also confirmed by the upfield chemical shifts (δC < 20 ppm) observed for both C-18 (δC 15.9) and C-19 (δC 14.9) [30]. Based on the above observations and the detailed analysis of other NOE correlations, the relative configuration of this compound was established. Furthermore, the absolute configuration of 1 at C-6 was determined by the modified Mosher’s esterification method [31,32]. The (S)- and (R)-MTPA esters of 1 (1a and 1b, respectively, as shown in Figure 4) were afforded by the reaction of 1 with (R)-(-) and (S)-(+)-MTPA chloride, respectively. Determination of the Δδ values (δS–δR) for protons nearing C-6 resulted in the establishment of the R configuration at C-6 in 1 (Figure 4). The absolute configuration of 1 was thus assigned as 2S,6R,11R,12R, mostly the same as that of isosarcophine (7) [24], except that of C-6. Therefore, cherbonolide A (1) was identified as 6-α-hydroxyisosarcophine.

Figure 3

Key NOESY correlations of 1.

Figure 4

1H NMR chemical shift differences Δδ (δS − δR) in ppm for the MTPA esters of 1 and 3.

The molecular formula of cherbonolide B (2) was determined to be C20H28O5 by the HRESIMS (m/z calcd 371.1830; found 371.1829, [M + Na]+), having one more oxygen than 1. Moreover, both 1 and 2 had almost identical 1H and 13C NMR data (Table 1), except for those of C-6. The allylic hydroxy group of 1 at C-6 was substituted by a hydroperoxyl in 2, with the characteristic signal of a broad singlet in the downfield region, δH 7.99 [26,33,34]. Obvious downfield shifts of C-6 (δC 65.2 in 1, 78.3 in 2) and H-6 (δH 4.70 in 1, 4.97 in 2) were also observed, indicating that 2 possesses the hydroperoxy group at C-6. Furthermore, reduction of 2 by reaction with triphenylphosphine afforded 1. On the basis of the above analyses, the planar structure and the (2S,6R,11R,12R)-configuration of 2 were determined.

Table 1

1H and 13C NMR chemical shifts for compounds 1–4.

Position	1		2		3		4
	δH, m (J in Hz) a	δC b, type	δH, m (J in Hz) a	δC b, type	δH, m (J in Hz) c	δC d, type	δH, m (J in Hz) c	δC d, type
1		160.7, C		160.6, C		160.6, C		159.8, C
2	5.44, dd(10.0, 1.6)	77.8, CH	5.43, dd(10.0, 1.6)	77.8, CH	4.91, dd(10.0, 1.6)	78.4, CH	4.98, d(10.4)	77.8, CH
3	4.90, d(10.0)	122.2, CH	4.91, d(10.0)	122.7, CH	4.55, d(10.0)	123.9, CH	4.45, d(10.4)	123.5, CH
4		141.6, C		140.8, C		140.2, C		139.4, C
5α	2.20, m	47.9, CH2	2.18, dd(12.4, 10.8)	42.6, CH2	2.42, dd(12.0, 3.2)	49.1, CH2	1.98, m	49.3, CH2
5β	2.76, dd(12.8, 5.2)		2.87, dd(12.4, 4.4)		189, m		2.25, dd(12.8, 3.6)
6	4.70, ddd(10.4, 10.4, 5.2)	65.2, CH	4.97, ddd(10.8, 9.2, 4.4)	78.3, CH	3.84, dd(9.2, 9.2)	69.6, CH	4.21, ddd(11.2, 9.2, 3.6)	64.8, CH
7	5.20, d (10.4)	128.1, CH	5.05, d (9.2)	123.1, CH	5.09, d (9.2)	131.6, CH	4.84, d (9.2)	131.2, CH
8		139.8, C		144.2, C		138.4, C		139.4, C
9α	2.03, m	36.8, CH2	2.07, m	36.9, CH2	2.21, ddd(13.6, 13.6, 2.4)	28.2, CH2	1.58, m	28.5, CH2
9β	2.38, m		2.42, m		1.63, m		2.30, dd(13.2, 4.8)
10α	1.29, m	23.5, CH2	1.35, m	23.6, CH2	1.16, m	23.9, CH2	1.60, m	22.7, CH2
10β	2.51, m		2.17, m		1.84, m		1.28, m
11	2.42, dd(10.8, 2.8)	61.4, CH	2.43, m	61.4, CH	2.24, dd(10.4, 2.4)	58.9, CH	1.98, m	62.6, CH
12		60.8, C		60.8, C		59.7, CH		60.9, C
13α	2.03, m	36.9, CH2	2.02, m	37.0, CH2	1.49, m	35.5, CH2	1.61, m	37.1, CH2
13β	1.06, m		1.07, m		0.98, m		0.65, m
14α	2.49, m	23.7, CH2	2.52, m	23.7, CH2	1.58, m	22.2, CH2	2.08, m	23.1, CH2
14β	2.01, m		2.03, m		1.58, m		1.65, m
15		123.8, C		123.8, C		123.8, C		123.7, C
16		174.4, C		174.4, C		173.9, C		174.3, C
17	1.86, s	8.8, CH3	1.86, s	8.7, CH3	1.64, s	8.8, CH3	1.63, s	8.8, CH3
18	1.70, s	15.9, CH3	1.72, s	15.9, CH3	1.31, s	18.1, CH3	1.28, s	16.9, CH3
19	1.86, s	14.9, CH3	1.89, s	15.3, CH3	1.45, s	22.2, CH3	1.32, s	21.8, CH3
20	1.33, s	15.8, CH3	1.33, s	15.8, CH3	1.00, s	17.1, CH3	0.99, s	16.4, CH3
6-OOH			7.99, br s

Spectrum recorded at 400 MHz in CDCl3. Spectrum recorded at 100 MHz in CDCl3. Spectrum recorded at 400 MHz in C6D6. Spectrum recorded at 100 MHz in C6D6.

Cherbonolide C (3) should have the same molecular formula as 1, according to HRESIMS data. Also, the 1H-1H COSY and HMBC correlations (Figure 2) of 3 are similar to those of 1, suggesting that these compounds possess almost the same molecular skeleton. Analysis of NOE correlations (Figure 5) showed that the relative configurations at C-2, C-11 and C-12 in 1 and 3 are the same. Assuming the β-orientation of H-2, as H3-18 showed NOE interaction with H-2 but not with H-3, the E geometry was assigned to the trisubstituted C-3/C-4 double bond. One of the methylene protons at C-5 (δH 2.42, dd, J = 12.0, 3.2 Hz) displayed NOE interaction with H-3, but not with H3-18, and was hence determined as H-5α. Further, H-6 (δH 3.84, dd, J = 9.2, 9.2 Hz) showed NOE correlations with H-5α and H-9α, but not with H-9β and H3-19. These observations, together with NOE correlations of H-9β/H3-19, H3-19/H-7 and H-7/H3-18, enabled deduction of the α-orientation of H-6 and led to the assignment of a 6S* configuration and a Z geometry of the trisubstituted C-7/C-8 double bond in 3. The olefinic methyl group attaching at C-8 showed carbon signal at δC 22.2 ppm further confirmed the Z geometry of C-7/C-8 double bond [30]. The absolute configuration of 3 at C-6 was also verified by using the modified Mosher’s method. Determination of the Δδ values (δS − δR, shown in Figure 4) for protons neighboring C-6 further confirmed the S configuration at C-6 in 3 (Figure 4). The absolute configuration of 3 was thus assigned as 2S,6S,11R and 12R. Thus, cherbonolide C (3) is the 7Z isomer of cherbonolide A (1).

Figure 5

Key NOESY correlations for 3 and 4.

Cherbonolide D (4) was found to be an isomer of 3 according to HRESIMS. Both compounds have almost the same 1H-1H COSY and HMBC correlations, indicating they have the same molecular skeleton. NMR data of 3 and 4 are nearly the same (Table 1), except for those of CH-6, suggesting that 4 could be the C-6 epimer of 3. The (2S,6R,11R,12R)-configuration and the E and Z geometries of C-3/C-4 and C-7/C-8 double bonds of 4, respectively, were also established by analysis of NOE correlations to be as those of 3 (Figure 5).

Cherbonolide E (5) was determined to have a molecular formula C20H28O5 from its HRESIMS data (m/z calcd 371.1830; found 371.1829, [M + Na]+), with one more oxygen than in 4. Compounds 4 and 5 displayed almost identical 1H and 13C NMR data (Table 2), except for those of CH-6. It was found that the hydroxy substituent of 4 at C-6 was replaced by a hydroperoxy group in 5, with the characteristic signal of a broad singlet at δH 7.25 [26,33,34]. The obvious downfield shifts of C-6 (δC 64.8 in 4, 78.9 in 5) and H-6 (δH 4.21 in 4, 4.58 in 5) also confirmed the substitution of a hydroperoxy group at C-6 of 5. Furthermore, reduction of 5 with triphenylphosphine could afford 4. Therefore, the structure of 5, with the (2S,6R,11R,12R)-configuration, was determined.

Table 2

1H and 13C NMR chemical shifts for compounds 5 and 6.

Position	5		6
	δH, m (J in Hz) a	δC b, type	δH, m (J in Hz) c	δC d, type		δH, m (J in Hz) c	δC d, type
1		160.4, C		141.4, C	1′		141.5, C
2	4.95, d (10.0)	78.4, CH	5.28, d (10.0)	82.7, CH	2′	5.50, d (10.0)	81.9, CH
3	4.42, d (10.0)	124.6, CH	5.06, d (10.0)	126.4, CH	3′	4.92, d (10.0)	125.1, CH
4		139.2, C		140.2, C	4′		141.0, C
5α	1.95, m	44.6, CH2	2.21, m	38.5, CH2	5′α	2.21, m	38.8, CH2
5β	2.47, br d (11.0)		2.32, m		5′β	2.31, m
6	4.58, ddd (11.0, 9.5, 2.5)	78.9, CH		24.2, CH2	6′		24.2, CH2
6α			2.07, m		6′α	2.07, m
6β			2.42, m		6′β	2.42, m
7	4.78, d (9.5)	126.6, CH	4.98, dd (9.2, 9.2)	125.6, CH	7′	4.95, dd (9.2, 9.2)	125.5, CH
8		143.8, C		133.1, C	8′		133.3, C
9α	1.65, m	29.8, CH2	1.96, m	36.6, CH2	9′α	1.96, m	36.6, CH2
9β	2.52, dd (14.0, 4.5)		2.27, m		9′β	2.27, m
10α	1.28, m	23.4, CH2	1.22, m	23.6, CH2	10′α	1.22, m	23.7, CH2
10β	1.62, m		2.04, m		10′β	2.04, m
11	1.97, m	63.3, CH	2.51, m	62.1, CH	11′	2.51, m	62.2, CH
12		61.5, C		61.2, CH	12′		61.3, C
13α	1.59, m	37.6, CH2	1.83, m	37.3, CH2	13′α	1.83, m	37.4, CH2
13β	0.64, m		0.95, m		13′β	0.95, m
14α	2.07, m	23.7, CH2	2.33, m	22.6, CH2	14′α	2.33, m	22.7, CH2
14β	1.61, m		1.81, m		14′β	1.81, m
15		124.3, C		124.9, C	15′		124.9, C
16		174.3, C	6.13, br s	114.3, C	16′	6.17, d (3.6)	114.4, CH
17	1.63, s	9.4, CH3	1.72, s	10.2, CH3	17′	1.73, s	10.2, CH3
18	1.29, s	17.3, CH3	1.58, s	14.6, CH3	18′	1.59, s	14.6, CH3
19	1.34, s	22.5, CH3	1.65, s	14.7, CH3	19′	1.65, s	14.7, CH3
20	0.98, s	16.9, CH3	1.27, s	15.7, CH3	20′	1.27, s	15.7, CH3
6-OOH	7.25, br s

Spectrum recorded at 500 MHz in C6D6. Spectrum recorded at 125 MHz in C6D6. Spectrum recorded at 400 MHz in CDCl3. Spectrum recorded at 100 MHz in CDCl3.

Bischerbolide peroxide (6) was afforded as a white powder with the molecular formula C40H58O6 from HRESIMS (m/z calcd 657.4124; found 657.4125, [M + Na]+), appropriate for twelve degrees of unsaturation. The 13C NMR spectroscopic data of 6 revealed the presence of 40 carbons (Table 2). The DEPT spectra of 6 showed the presence of eight methyls, twelve sp methylenes, six sp oxygenated methines, four sp methines, two sp and eight sp nonprotoned carbons (including two ester carbonyls). NMR signals resonating at δC 114.3 (CH), 141.4 (C), 124.9 (C), 82.7 (CH) and 10.2 (CH3), and δH 5.28 (1H, d, J = 10.0 Hz) and δH 1.72 (3H, s), and another group of signals observed at δC 114.4 (CH), 141.5 (C), 124.9 (C), 81.9 (CH) and 10.2 (CH3), and δH 5.50 (1H, d, J = 10.0 Hz) and δH 1.73 (3H, s), revealed the presence of two slightly different 2,5-dihydrofuran rings with a peroxyl group by comparison of the similar NMR data of five-membered rings in the literature [35]. Also, two groups of signals resonating at δC 61.2 (C), 62.1 (CH) and δH 2.51 (1H, m), and δC 61.3 (C), 62.2 (CH) and δH 2.51 (1H, m) showed the presence of two trisubstituted epoxides. Four trisubstituted olefinic bonds were revealed from NMR signals appearing at δC 126.4 (CH), δC 140.2 (C) and δH 5.06 (1H, d, J = 10.0 Hz); at δC 125.6 (CH), δC 133.1 (C) and δH 4.98 (1H, dd, J = 9.2, 9.2 Hz); at δC 125.1 (CH), δC 141.0 (C) and δH 4.92 (1H, dd, J = 10.0 Hz); and at δC 125.5 (CH), δC 133.3 (C) and δH 4.95 (1H, dd, J = 9.2, 9.2 Hz), respectively.

As the 13C NMR spectrum of 6 was constituted by twenty sets of signals with each set containing two peaks of very similar chemical shifts, 6 was thus identified as a compound formed from the connection of two quite similar diterpenoid subunits. The entire planar structure was established by examination of 1H and 13C NMR data and 1H-1H COSY and HMBC correlations (Figure 6). Two methines resonating at δC 114.3 and δC 114.4 were considered to be the positions at which the two cembranoidal units were connected by insertion of a peroxyl group. Based on the above analyses, the molecular skeleton of 6 was elucidated as the biscembranoid formed by the connection of two molecules of isosarcophytoxide [36] via a peroxyl group at C-16 and C-16′. The fragmentation pattern of ESIMS (Figure 7) could further prove the dimeric nature of 6 and the peroxyl linkage at C-16/C-16′. One ion peak displayed at m/z 339 can be explained by the cleavage of O–O bond and the following elimination of H-16 from a monocembranoidal unit in 6 to form a sodiated cembranoid lactone molecular ion A (pathway a). The other ion peaks can be interpreted by the cleavage of the single bond between C-16 and peroxyl oxygen to afford ion B (m/z 301), and a peroxycembranoidal radical which could further abstract an hydrogen atom and form the sodium adduct C (m/z 357) (pathway b). Moreover, compound 6 was found to be the first example of a biscembranoid with a molecular skeleton formed by two cembranoid units connected by a peroxyl group.

Figure 6

Selected 1H-1H COSY and HMBC correlations of 6.

Figure 7

ESIMS fragmentation of 6.

The relative configuration of 6 was determined from a literature survey [36,37] and NOE correlations (Figure 8). The 13C NMR spectrum of 6 displayed 40 signals of two sets signals with nearly identical chemical shifts, representing the very similar stereochemical environments of the two structural units. In addition, compound 6 was found to have nearly identical chemical shifts for H-11 (11′), H3-18 (18′), H3-20 (20′) and C-20 (20′) to those of (2S,11R,12R)-isosarcophytoxide (8), and were in turn found to exhibit distinguishable differences to the corresponding chemical shifts of (2R,11R,12R)-isosarcophytoxide (9) (Table 3 and Figure 9). Thus, 6 possessed the cembranoidal structural unit derived from 8, as also proven by observed NOE correlations (Figure 8). Different proton values were observed for H-2 (δH 5.28) and H-2′ (δH 5.50), indicating that H-2′ was on the same planar face as the peroxide group and was deshielded, and H-2 was on the same planar face as H-16 and was shielded. As compounds 1−7 were isolated from the same organism in this study, they are likely to possess the same absolute S,R,R-configurations at the chiral centers C-2, C-11 and C-12, respectively, as those of 1 and 3. A previous report also showed that different absolute configurations at C-2 of the related diasteromeric dihydrofuran ring-containing cembranoids could significantly influence the sign of the specific optical rotation [36,38]. For cembranoids with 2S configuration a significant positive and for those with 2R configuration a negative optical rotation were found. The of 6 was +41; thus, the absolute configuration of 6 was deduced to be 2S,11R,12R,16R, 2′S,11′R,12′R,16′S.

Figure 8

Selected NOESY correlations for 6.

Table 3

Selected 1H and 13C NMR data comparison with 6, (2S,11R,12R)-isosarcophytoxide (8) and (2R,11R,12R)-isosarcophytoxide (9).

Position	6	8 a	9 a
H-11	δH 2.51 (H-11, H-11′)	δH 2.50	δH 2.75
C-11	δC 62.1 (C-11)	δC 62.3	δC 61.2
	δC 62.2 (C-11′)
C-12	δC 61.2 (C-12)	δC 61.4	δC 60.7
	δC 61.3 (C-12′)
C-13	δC 37.3 (C-13)	δC 37.4	δC 35.4
	δC 37.4 (C-13′)
C-14	δC 22.6 (C-14)	δC 22.5	δC 20.4
	δC 22.7 (C-14′)
H3-18	δH 1.58 (H3-18)	δH 1.58	δH 1.70
	δH 1.59 (H3-18′)
H3-20	δH 1.27 (H3-20, H3-20′)	δH 1.28	δH 1.18
C-20	δC 15.7 (C-20, C-20′)	δC 15.7	δC 17.7

The selected 1H and 13C data were cited from ref. [36,37].

Figure 9

Structures of (2S,11R,12R)-isosarcophytoxide (8) and (2R,11R,12R)-isosarcophytoxide (9) [36].

The plausible biosynthesis of 6 might arise from the proton abstraction at C-16 of 8 by hydrogen peroxide radical HOO• to form a radical intermediate 10, which could react with O2 from one plane side of radical center C-16 to afford cembranoidal peroxide radical 11. Further reaction of 11 with 10 from another side could lead to the formation of 6 (Scheme 1). However, the possibility that 6 might be generated by autooxidation of 8 could not be neglected.

Scheme 1

Proposed biosynthetic pathway for 6.

It is known that the proteolytic enzymes and toxic reactive oxygen species produced by stimulated neutrophils might play a critical role in the pathogenesis of many inflammatory diseases [39,40]. By measuring the capability to inhibit N-formyl-methionyl-leucyl-phenylalanine/cytochalasin B (fMLF/CB)-induced superoxide anion generation and elastase release in human neutrophils, the in vitro anti-inflammatory effects for metabolites 1–7 were evaluated [41,42]. According to the results (shown in Table 4), compound 6 had a significant inhibitory effect (64.6 ± 0.8%), with an IC50 value of 26.2 ± 1.0 μM, on the generation of superoxide anions, and compounds 1 and 3 had moderate inhibitory effects (32.1 ± 4.3% and 44.5 ± 4.6%, respectively) at 30 μM. Compounds 1, 3 and 6 revealed moderate inhibitory effects (37.6 ± 5.0%, 35.6 ± 6.2% and 42.4 ± 5.1%, respectively) on elastase release at the same concentration. These results, obtained after stimualting the neutrophils with fMLF/CB, may suggest that 1, 3 and 6 have potential merits against inflammatory disorders.

Table 4

Inhibitory effects of compounds 1–7 on superoxide anion generation and elastase release in fMLF/CB-induced human neutrophils.

Compounds	Superoxide Anion		Elastase Release
	IC50 (μM) a	Inh b %	Inh b %
1	>30	32.1 ± 4.3 **	37.6 ± 5.0 **
2	>30	4.0 ± 6.7	23.5 ± 6.6 *
3	>30	44.5 ± 4.6 ***	35.6 ± 6.2 **
4	>30	6.4 ± 4.2	27.6 ± 6.4 **
5	>30	2.6 ± 6.2	30.5 ± 4.6 **
6	26.2 ± 1.0	64.6 ± 0.8 ***	42.4 ± 5.1 **
7	>30	3.5 ± 5.3	20.7 ± 4.1 **
Idelalisib	0.07 ± 0.01	102.8 ± 2.2 ***	99.6 ± 4.2

a Concentration necessary for 50% inhibition (IC50). b Percentage of inhibition (Inh %) at 30 μM. Data are presented as mean ± S.E.M. (n = 3–4); * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the control value.

In summary, examination of the chemical constituents of the soft coral Sarcophyton cherbonnieri led to the discovery of six new compounds 1–6, along with one known compound 7. Although a number of natural compounds possessing a peroxyl group, such as artemisinin [43], neovibsanin C [44], cardamom peroxide [45], plakortin [46] and chondrillin [47], have been discovered, compound 6 was discovered to be the first compound with a molecular skeleton consisting of two cembranoidal units connected by a peroxide group. Similar to the results of previous studies indicating that natural peroxides could possess promising biological activity [48], compound 6 was found to possess anti-inflammatory activity by exhibiting stronger ability on inhibition on the generation of superoxide anions and release of elastase in fMLF/CB-induced human neutrophils.

3. Materials and Methods

3.1. General Procedures

The values of optical rotation of the metabolites were determined with a JASCO P-1020 polarimeter (JASCO Corporation, Tokyo, Japan). Infrared absorptions were recorded using a JASCO FT/IR-4100 infrared spectrophotometer (JASCO Corporation, Tokyo, Japan). 1H and 13C NMR spectra were obtained on a Varian 400MR FT-NMR (or Varian Unity INOVA500 FT-NMR) instrument (Varian Inc., Palo Alto, CA, USA) at 400 MHz (or 500 MHz) and 100 MHz (or 125 MHz), respectively, in CDCl3 or C6D6. The data of LRESIMS and HRESIMS were measured using a Bruker APEX II (Bruker, Bremen, Germany) mass spectrometer. Silica gel (230–400 mesh) was used as adsorbent for column chromatography. TLC analyses were performed using precoated silica gel plates (Kieselgel 60 F-254, 0.2 mm) (Merck, Darmstadt, Germany). Further purification of impure fractions or compounds were performed by high-performance liquid chromatography on a Hitachi L-7100 HPLC instrument (Hitachi Ltd., Tokyo, Japan) with a Merck Hibar Si-60 column (250 mm × 21 mm, 7 μm; Merck, Darmstadt, Germany) and on a Hitachi L-2455 HPLC apparatus (Hitachi, Tokyo, Japan) with a Supelco C18 column (250 mm × 21.2 mm, 5 μm; Supelco, Bellefonte, PA, USA).

3.2. Animal Material

The soft coral S. cherbonnieri was collected by hand using scuba diving from Jihui Fish Port, Taiwan, in March 2013, at a depth of 10–15 m. Organisms of the marine animal were stored in a freezer until extraction.

3.3. Extraction and Isolation

The frozen marine organisms, S. cherbonnieri (1.2 kg, wet wt), were freeze-dried (yield: 207 g), minced to small pieces and then extracted thoroughly with EtOAc (1 L × 5). The combined EtOAc extract (10.2 g) was concentrated under reduced pressure to yield a residue, which was chromatographed over a silica gel column by eluting with acetone in n-hexane (0–100%, stepwise), and then with MeOH in acetone (0–100%, stepwise) to yield 19 fractions. Fraction 9, eluting with n-hexane–acetone (6:1), was repeatedly purified by column chromatography over silica gel to yield a solid which was immersed in cold MeOH (0 °C) to afford a white powder 6 (24.3 mg). Fraction 10, eluting with n-hexane–acetone (4:1), was further purified over silica gel using n-hexane–acetone (6:1) to afford seven subfractions (A1–A7) and afford 7 (320.4 mg). Subfraction A2 was further separated by reverse-phase HPLC using acetonitrile–H2O (1:1.3) to afford 1 (11.0 mg). Subfraction A3 was purified by reverse-phase HPLC using acetonitrile–H2O (1:1.1) to afford 2 (13.3 mg) and 5 (10.1 mg). Fraction 13, eluting with n-hexane–acetone (1:1), eluting with acetone by sephadex LH-20 to afford five subfractions (B1–B5). Subfraction B3 was purified by reverse-phase HPLC using acetonitrile–H2O (1:1.4) to afford 3 (10.6 mg) and 4 (9.4 mg).

3.3.1. Cherbonnolide A (1)

Colorless oil; +43 (c 1.00, CHCl3); IR (neat) νmax 3444, 1746, and 1003 cm−1; 13C and 1H NMR data see Table 1; HRMS (ESI-TOF) m/z: [M + Na]+ Calcd for C20H28O4Na 355.1880; Found 355.1879.

3.3.2. Cherbonnolide B (2)

Colorless oil; +59 (c 1.00, CHCl3); IR (neat) νmax 3363, 1741, 1678, and 1093 cm−1; 13C and 1H NMR data see Table 1; HRMS (ESI-TOF) m/z: [M + Na]+ Calcd for C20H28O5Na 371.1829; Found 371.1830.

3.3.3. Cherbonnolide C (3)

Colorless oil; +26 (c 1.00, CHCl3); IR (neat) νmax 3445, 1749, 1678, and 1094 cm−1; 13C and 1H NMR data see Table 1; HRMS (ESI-TOF) m/z: [M + Na]+ Calcd for C20H28O4Na 355.1880; Found 355.1882.

3.3.4. Cherbonnolide D (4)

White powder; +3 (c 1.00, CHCl3); IR (neat) νmax 3445, 1748, and 1096 cm−1; 13C and 1H NMR data see Table 1; HRMS (ESI-TOF) m/z: [M + Na]+ Calcd for C20H28O4Na 355.1880; Found 355.1883.

3.3.5. Cherbonnolide E (5)

Colorless oil; +8 (c 1.00, CHCl3); IR (neat) νmax 3389, 1748, 1678 and 1096 cm−1; 13C and 1H NMR data see Table 2; HRMS (ESI-TOF) m/z: [M + Na]+ Calcd for C20H28O5Na 371.1829; Found 371.1830.

3.3.6. Bischerbolide Peroxide (6)

White powder; +41 (c 1.00, CHCl3); IR (neat) νmax 3420, 1733, 1232, 1166 and 1040 cm−1; 13C and 1H NMR data see Table 2; HRMS (ESI-TOF) m/z: [M + Na]+ Calcd for C40H58O6Na 657.4125; Found 657.4124.

3.3.7. Reduction of Cherbonolides B and E (2 and 5)

In diethyl ether (5.0 mL), compound 2 (3.2 mg) was added followed by addition of excess amount of triphenylphosphine (2.9 mg) and the mixture was stirred at room temperature for 4 h. The solvent of the solution was evaporated under reduced pressure to afford a residue, which was purified by silica gel column chromatography using n-hexane–acetone (3:1) as an eluent to yield 1 (2.9 mg, 95%). Similarly, 5 (2.1 mg) was converted to 4 (1.7 mg) in 85% yield.

3.3.8. Preparation of (S)- and (R)- MTPA Esters of 1 and 3

Compound 1 (1.3 mg) was dissolved in pyridine 100 μL and added (R)-(−)-α-methoxy-α-(trifluoromethyl) phenylacetyl chloride (MTPA chloride) 10 μL. The mixture was permitted to stand at room temperature overnight and the reaction was found to complete by monitoring with normal-phase TLC plate. The solution was dried completely under the vacuum of an oil pump and the residue was purified by a short silica gel column using acetone to n-hexane (1:3) to yield the (S)-MTPA ester 1a (0.9 mg, 62.9%). The same procedure was applied to obtain the (R)-MTPA ester 1b (1.0 mg, 69.9%) from the reaction of (S)-(+)-α-methoxy-α-(trifluoromethyl) phenylacetyl chloride with 1 in pyridine. Selective 1H NMR (CDCl3, 400 MHz) data of 1a: δH 4.925 (1H, d, J = 10.0 Hz, H-3), 1.769 (3H, s, H3-18), 2.821 (1H, dd, J = 12.8, 4.8 Hz, H-5a), 2.376 (1H, m, H-5b), 5.107 (1H, d, J = 9.6 Hz, H-7), 1.880 (3H, s, H3-19), 2.460 (1H, m, H-9a), 1.989 (1H, m, H-9b); selective 1H NMR (CDCl3, 400 MHz) data of 1b: δH 4.905 (1H, d, J = 10.0 Hz, H-3), 1.763 (3H, s, H3-18), 2.739 (1H, dd, J = 12.8, 5.6 Hz, H-5a), 2.267 (1H, m, H-5b), 5.217 (1H, d, J = 9.6 Hz, H-7), 1.905 (3H, s, H3-19), 2.476 (1H, m, H-9a), 1.998 (1H, m, H-9b).

Preparation of (S)- and (R)- MTPA esters of 3 used the same reaction and purification procedures as the reduction of 1, the solution of 3 (1.1 mg) was converted to the (S)-MTPA ester 3a (0.8 mg) in 74% yield and (R)-MTPA ester 3b (0.9 mg) in 80% yield, respectively. Selective 1HNMR (C6D6, 400 MHz) data of 3a: δH 1.243 (3H, s, H3-18), 2.311 (1H, dd, J = 11.2, 2.4 Hz, H-5a), 1.930 (1H, dd, J = 11.2, 11.2 Hz, H-5b), 5.065 (1H, d, J = 9.2 Hz, H-7), 1.416 (3H, s, H3-19); selective 1H NMR (C6D6, 400 MHz) data of 3b: δH 1.244 (3H, s, H3-18), 2.363 (1H, dd, J = 12.0, 3.2 Hz, H-5a), 1.982 (1H, dd, J = 12.0, 11.6 Hz, H-5b), 4.9515 (1H, d, J = 10.0 Hz, H-7), 1.360 (3H, s, H3-19).

3.4. In Vitro Anti-Inflammatory Testing

3.4.1. Human Neutrophils

Blood was obtained from elbow vein of healthy adult volunteers (years 20–30). Neutrophils were enriched by dextran sedimentation, Ficoll-Hypaque centrifugation, and hypotonic lysis. Neutrophils were incubated in an ice-cold Ca2+-free HBSS buffer (pH 7.4) [42].

3.4.2. Superoxide Anion Generation

Neutrophils (6 × 105 cells mL−1) incubated in HBSS with ferricytochrome c (0.5 mg mL−1) and Ca2+ (1 mM) at 37 °C were treated with DMSO (as control) or compound for 5 min. Neutrophils were primed by cytochalasin B (CB, 1 μg mL−1) for 3 min before activating fMLF (100 nM) for 10 min (fMLF/CB) [40,49].

3.4.3. Elastase Release

Neutrophils (6 × 105 cells mL−1) incubated in HBSS with MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide (100 μM) and Ca2+ (1 mM) at 37 °C were treated with DMSO or compound for 5 min. Neutrophils were activated with fMLF (100 nM)/CB (0.5 μg mL−1) for 10 min [40].

3.4.4. Statistical Analysis

Data are displayed as the mean ± SEM and comparisons were performed by Student’s t-test. A probability value of 0.05 or less was considered to be significant. The software Sigma Plot (version 8.0, Systat Software, San Jose, CA, USA) was used for the statistical analysis.

4. Conclusions

Six new cembranoids, cherbonolides A−E (1–5) and a cembrane dimer (bischerbolide peroxide, 6), along with isosarcophine (7) were isolated from the Formosan soft coral Sarcophyton cherbonnieri. Bischerbolide peroxide (6) was discovered as the first example of cembranoid dimers possessing a peroxide group as a linking group. Compounds 1, 3 and 6 showed an anti-inflammatory activity through their inhibitory effects on the generation of superoxide anion in fMLF/CB-induced human neutrophils. Moreover, peroxide 6 was also shown to exhibit stronger activity in inhibiting the elastase release which supported its anti-inflammatory activity.