New Biscembranoids Sardigitolides A-D and Known Cembranoid-Related Compounds from Sarcophyton digitatum: Isolation, Structure Elucidation, and Bioactivities.

Chemical examination from the cultured soft coral Sarcophyton digitatum resulted in the isolation and structural identification of four new biscembranoidal metabolites, sardigitolides A-D (1-4), along with three previously isolated biscembranoids, sarcophytolide L (5), glaucumolide A (6), glaucumolide B (7), and two known cembranoids (8 and 9). The chemical structures of all isolates were elucidated on the basis of 1D and 2D NMR spectroscopic analyses. Additionally, in order to discover bioactivity of marine natural products, 1-8 were examined in terms of their inhibitory potential against the upregulation of inflammatory factor production in lipopolysaccharide (LPS)-stimulated murine macrophage J774A.1 cells and their cytotoxicities against a limited panel of cancer cells. The anti-inflammatory results showed that at a concentration of 10 µg/mL, 6 and 8 inhibited the production of IL-1β to 68 ± 1 and 56 ± 1%, respectively, in LPS-stimulated murine macrophages J774A.1. Furthermore, sardigitolide B (2) displayed cytotoxicities toward MCF-7 and MDA-MB-231 cancer cell lines with the IC50 values of 9.6 ± 3.0 and 14.8 ± 4.0 µg/mL, respectively.

1. Introduction

Cembrane diterpenoids and their derivatives are an abundant and structurally diverse group of secondary metabolites of soft corals and gorgonians. The 14-membered ring structures of cembranoids are biosynthetically formed by cyclization of a geranylgeranyl pyrophosphate precursor between carbons 1 and 14 [1]. These compounds possess a defense mechanism against their natural predators and protect corals against viral infections [2]. Cembranoids exhibit various useful biological properties, such as antitumor [3,4,5,6,7,8] and anti-inflammatory activities [7,9,10,11,12].

Previous studies indicated that species of Sarcophyton are rich sources of compounds, such as cembranoids [13,14,15,16], biscembranoids [17,18,19,20,21], and steroids [22]. Until now, more than 500 marine natural objects have been found from the soft corals of the genus Sarcophyton [23]. It is worth mentioning that biscembranoid-type metabolites are mainly biosynthetically rationalized by a Diels–Alder reaction of two monocembranoidal members, a 1,3-diene cembranoid unit, and a dienophile cembranoid unit. The diene unit forms new trisubstituted conjugated double bonds at C-34/C-35. Normally, most biscembranoids are biosynthesized by the endo-type cyclization of a Diels–Alder reaction [21]. Although many specimens of Sarcophyton genus have been chemically studied, there is only one chemical investigation of the soft coral Sarcophyton digitatum that has been reported by Zeng et al. in 2000, which led to the isolation of a polyhydroxylated sterol, sardisterol, from this species [24]. Due to the structure diversity and attracting bioactivities of natural products from soft corals, it is well worth further investigating for the discovery of new metabolites from S. digitatum. As part of our continuing investigations of discovering bioactive compounds from soft corals, herein is reported the chemical examination of a cultured S. digitatum, which resulted in the isolation and structure identification of four new biscembranoidal metabolites, sardigitolides A–D (1–4), along with five known compounds, sarcophytolide L (5) [25], glaucumolide A (6) [18], glaucumolide B (7) [18], isosarcophytonolide D (8) [26], and (4Z,8S,9S,12Z,14E)-9-hydroxy-1-isopropyl-8,12-dimethyloxabicyclo [9.3.2]-hexadeca-4,12,14-trien-18-one (9) [27]. Lipopolysaccharide (LPS), a known endotoxin, is the main component of the outer membrane of gram-negative bacteria, which could induce an inflammatory response in mammalian tissues. In most cases, the inflammatory response helps the organism to clear the LPS or pathogen. However, the violent inflammation caused by overproduced LPS could lead to tissue damage [28]. In the present study, the inhibitory effect of isolates 1–8 against the enhanced production of inflammatory factors in LPS-treated murine macrophage J774A.1 cells was examined. Furthermore, the in vitro cytotoxicities toward MCF-7, MDA-MB-231, HepG2, and HeLa cells of compounds 1–8 were investigated.

2. Results and Discussion

Soft coral Sarcophyton digitatum was cultured in the National Museum of Marine Biology & Aquarium. A frozen sample was initially extracted with n-hexane and subsequently by ethyl acetate. The ethyl acetate layer was concentrated and repeatedly chromatographed to isolate compounds 1–9 (Figure 1), and structure of new compounds 1–4 were established based on spectroscopic analyses (Supplementary Materials S1–S40).

Figure 1

Structures of compounds 1–9.

Sardigitolide A (1) was isolated as a white amorphous powder. High-resolution electron spray ionization mass spectroscopy (HRESIMS) showed a molecular ion peak at m/z 721.4287 corresponding to the molecular formula and implying 11 degrees of unsaturation. In the IR spectrum, a characteristic absorption at 3433 cm−1 indicated the presence of a hydroxy group. The 13C spectrum (Table 1) showed 41 carbon signals, including 9 methyl groups, 11 methylene, 10 methines, and 11 quaternary carbons. Its 1H (Table 2) and 13C NMR spectra revealed signals of three olefinic methyl groups (δH 1.89, s; 1.86, s; 1.70, s and δC 26.6; 19.3; 17.9, respectively), two methyl groups linked to oxygen-bearing quaternary carbons (δH 1.16, s; 1.13, s and δC 23.7; 25.3, respectively), one methoxy group (δH 3.55, s; δC 51.3), an isopropyl group (δH 2.19, m; 0.95, d, J = 6.8 Hz; 0.76, d, J = 6.8 Hz and δC 29.1, CH; 21.1, C; 18.3, C, respectively), two trisubstituted double bond (δH 6.44, s; δC 126.0, CH; 160.9, C and δH 5.06, d, J = 10.0 Hz; 125.2, CH; 138.4, C, respectively), one tetrasubstituted double bond (δC 131.7, C and 130.6, C), three oxygen-bearing methines (δH 4.86, t, J = 6.4 Hz; 3.49, m; 3.44, dd, J = 4.0, 12.0 Hz and δC 67.8, CH; 81.7, CH; 73.8, CH, respectively), two oxygen-bearing quaternary carbons (δC 73.8, C and 70.4, C), and four carbonyl carbons (δC 214.2, C; 210.0, C; 202.5, C; and 174.9, C). All of the above evidence suggested that 1 contained a biscembranoid skeleton. The planar structure of 1 was further determined. The correlations spectroscopy (COSY) signals revealed eight different structural units from H-2 to H2-36; H2-6 via H2-7 and H2-8; H-9 to H3-18; isopropyl protons H3-16 and H3-17 via H-15, H-12, and H-11; H-21 to H-22; H2-24 via H2-25 and H2-26; H2-28 via H2-29 and H-30; and H2-32 to H-33. These units were assembled by heteronuclear multiple bond correlations (HMBCs) of H3-16 to C-12, C-15, and C-17; H3-18 to C-8, C-9, and C-10; H3-19 to C-4, C-5, and C-6; H3-37 to C-34, C-35, and C-36; H3-38 to C-22, C-23, and C-24; H3-39 to C-27, C-28, and C-29; H3-40 to C-30, C-31, and C-32; H-2 to C-1, C-3, and C-14; H2-11 to C-10; H-12 to C-13; H2-14 to C-1 and C-20; and H-33 to C-34 and C-35. Accordingly, compound 1 had an additional degree of unsaturation, which was suggested to be of an ether ring between C-26 and C-30 established via an HMBC from H-26 to C-30. The gross structure of 1 was thus confirmed as shown in Figure 2 which possesses a biscembranoid skeleton similar to ximaolides E and F [29].

Table 1

13C NMR spectroscopic data of 1–4.

Position	1 α	2	3	4 b
1	50.2 (C)	48.7 (C)	49.8 (C)	49.4 (C)
2	46.0 (CH) c	47.3 (CH)	45.8 (CH)	44.0 (CH)
3	202.5 (C)	203.8 (C)	210.3 (C)	201.5 (C)
4	126.0 (CH)	126.0 (CH)	51.0 (CH2)	124.7 (CH)
5	160.9 (C)	159.8 (C)	143.2 (C)	159.4 (C)
6	32.3 (CH2)	39.8 (CH2)	36.5 (CH2)	34.0 (CH2)
7	24.7 (CH2)	24.1 (CH2)	25.7 (CH2)	25.2 (CH2)
8	31.0 (CH2)	31.1 (CH2)	33.8 (CH2)	33.6 (CH2)
9	44.6 (CH)	45.7 (CH)	47.7 (CH)	44.7 (CH)
10	214.2 (C)	213.3 (C)	213.6 (C)	213.6 (C)
11	34.7 (CH2)	35.1 (CH2)	34.2 (CH2)	37.1 (CH2)
12	52.6 (CH)	52.1 (CH)	51.1 (CH)	52.3 (CH)
13	210.0 (C)	211.2 (C)	211.6 (C)	207.6 (C)
14	46.6 (CH2)	47.7 (CH2)	46.8 (CH2)	45.2 (CH2)
15	29.1 (CH)	29.4 (CH)	29.2 (CH)	28.3 (CH)
16	18.3 (CH3)	17.9 (CH3)	18.4 (CH3)	17.3 (CH3)
17	21.1 (CH3)	20.8 (CH3)	21.0 (CH3)	21.0 (CH3)
18	17.2 (CH3)	16.3 (CH3)	17.6 (CH3)	18.3 (CH3)
19	26.6 (CH3)	20.3 (CH3)	114.9 (CH2)	26.4 (CH3)
20	174.9 (C)	174.4 (C)	175.0 (C)	174.1 (C)
21	42.9 (CH)	40.8 (CH)	42.5 (CH)	41.7 (CH)
22	125.2 (CH)	124.0 (CH)	124.5 (CH)	126.8 (CH)
23	138.4 (C)	140.2 (C)	139.3 (C)	134.9 (C)
24	36.7 (CH2)	38.5 (CH2)	37.6 (CH2)	34.9 (CH2)
25	25.8 (CH2)	26.8 (CH2)	26.3 (CH2)	24.4 (CH2)
26	81.7 (CH)	84.8 (CH)	82.7 (CH)	79.8 (CH)
27	70.4 (C)	70.1 (C)	70.4 (C)	69.0 (C)
28	33.6 (CH2)	32.0 (CH2)	33.2 (CH2)	33.6 (CH2)
29	19.5 (CH2)	19.8 (CH2)	19.9 (CH2)	19.2 (CH2)
30	73.8 (CH)	74.0 (CH)	73.9 (CH)	72.8 (CH)
31	73.8 (C)	74.0 (C)	73.9 (C)	72.8 (C)
32	40.2 (CH2)	41.2 (CH2)	40.6 (CH2)	39.8 (CH2)
33	67.8 (CH)	69.9 (CH)	68.5 (CH)	64.9 (CH)
34	131.7 (C)	131.5 (C)	132.8 (C)	132.7 (C)
35	130.6 (C)	130.2 (C)	130.1 (C)	125.2 (C)
36	34.0 (CH2)	34.0 (CH2)	34.2 (CH2)	32.9 (CH2)
37	19.3 (CH3)	19.8 (CH3)	19.6 (CH3)	16.8 (CH3)
38	17.9 (CH3)	18.9 (CH3)	18.5 (CH3)	17.2 (CH3)
39	25.3 (CH3)	25.4 (CH3)	25.4 (CH3)	26.2 (CH3)
40	23.7 (CH3)	22.9 (CH3)	23.4 (CH3)	24.7 (CH3)
41	51.3 (CH3)	51.6 (CH3)	51.3 (CH3)	50.6 (CH3)

Spectroscopic data of 1–3 were recorded at 100 MHz in CDCl3. Spectroscopic data of 4 was recorded at 125 MHz in DMSO- d6. Attached protons were deduced by DEPT experiments.

Table 2

1H NMR spectroscopic data of 1–4.

Position	1 α	2	3	4 b
2	3.59, m	3.73, m	3.74, t (7.6) c	3.37, m
4	6.44, s	5.85, s	3.20, m; 3.64 m	6.30, s
6	1.68, m; 3.30, m	1.99, m; 2.21, m	1.80, m; 2.16 m	2.11, m
7	1.34, m; 1.52, m	1.51, m; 1.60, m	1.27, m	1.10, m; 1.30, m
8	1.34, m; 1.64, m	1.49, m	1.52, m; 1.63 m	1.23, m; 1.54, m
9	2.71, m	2.24, m	2.43, m	2.73, m
11	2.13, m; 2.76, m	2.09, m; 2.98, m	2.04, m; 3.0, m	2.05, m; 3.01, m
12	2.97, m	2.82, m	3.05, m	2.99, m
14	2.84, m; 3.11, m	2.58, m; 3.23, m	3.10, m	2.58, m; 2.82, m
15	2.19, m	2.07, m	2.03, m	2.03, m
16	0.76, d (6.8)	0.77, d (6.8)	0.79, d (6.8)	0.59, d (7.0)
17	0.95, d (6.8)	0.94, d (6.8)	0.95, d (6.4)	0.90, d (6.5)
18	1.08, d (7.2)	1.13, d (6.8)	1.13, d (7.2)	0.96, (7.0)
19	1.89, s	2.12, s	4.72, 4.88, brs	1.84, s
21	3.37, m	3.73, d (10.8)	3.50, m	3.36, m
22	5.06, d (10.0)	5.25, d (10.8)	5.17, d (10.4)	4.99, d (10.5)
24	1.98, m; 2.43, m	1.94, m; 2.52, m	1.94, m; 2.50 m	1.82, m; 2.24, m
25	1.64, m; 1.84, m	1.61, m; 1.99, m	1.63, m; 1.94 m	1.58, m; 1.64, m
26	3.49, m	3.60, m	3.54, d (9.6)	3.28, d (10.5)
28	1.52, m; 1.72, m	1.52, m; 1.71, m	1.58, m; 1.72 m	1.62, m; 2.16, m
29	1.57, m; 1.70, m	1.70, m;	1.63, m; 1.71 m	1.29, m; 1.64, m
30	3.44, dd (4.0; 12.0)	3.63, m	3.52, m	3.15, dd (4.0; 12.0)
32	1.55, m; 2.19 m	1.71, m; 1.92, m	1.60, m; 2.03 m	2.19, m
33	4.86, t (6.4)	4.80, t (6.4)	4.83, t (6.4)	4.78, d (10.5)
36	2.26, m	2.26 m; 2.56 m	2.15, m; 2.40, m	1.92, m
37	1.86, s	1.91, s	1.89, s	1.66, s
38	1.70, s	1.77, s	1.75, s	1.57, s
39	1.13, s	1.13, s	1.14, s	0.96, s
40	1.16, s	1.14, s	1.15, s	1.01, s
41	3.55, s	3.58, s	3.58, s	3.40, s

Spectroscopic data of 1–3 were recorded at 400 MHz in CDCl3. Spectroscopic data of 4 was recorded at 500 MHz in DMSO-d6. J values (in Hz) in parentheses.

Figure 2

Selected COSY and HMBC correlations of 1–3.

The relative configuration of 1 was determined by the analysis of correlations recorded by nuclear Overhauser effect spectroscopy (NOESY). As shown in Figure 3, the NOE correlations of H-4 (δH 6.44, s) with H3-19 (δH 1.89, s), together with the obviously downfield-shifted methyl group at C-19 (δC 26.6, CH3), suggested a cis geometry of C-4/C-5 trisubstituted double bond. Assuming the β-orientations of H3-41 as previously reported [30], NOE correlations of H3-41 with both of H3-16 (δH 0.76, d, J = 6.8 Hz) and H3-17 (δH 0.95, d, J = 6.8 Hz), and all of H3-16, H3-17 and H3-18 (δH 1.08, d, J = 7.2 Hz) with H-11 (δH 2.13, m) suggested the β-orientations of H3-18 and C-1 carbomethoxy groups, on the basis that metabolites of this type with absolute configurations determined previously all possessed 1S and 2S configurations [30]. Furthermore, the correlations of H-2 with H-22 (δH 5.06, d, J = 10.0 Hz); H-22 with H-24β (δH 2.43, m); and H-24β with H-25β (δH 1.84, m); H-25β with H3-39 (δH 1.13, s); H3-39 with H-28β (δH 1.52, m); H-28β with H-30 (δH 3.44, dd, J = 4.0, 12.0 Hz) reflected the β-orientation of H-30 and H3-39. In contrast, the α-orientations of H-9 and H-12 were confirmed by the NOE correlations of H-11α (δH 2.76, m) with both H-9 (δH 2.71, m) and H-12 (δH 2.97, m). The α-orientation of H-26 was determined by the NOE correlations of H-21 (δH 3.37, m) with H3-38 (δH 1.70, s); H3-38 with H-24α (δH 1.98, m); H-24α with H-25α (δH 1.64, m); H-25α with H-26 (δH 3.49, m). Also, the α-orientations of H-33 and H3-40 were determined by the NOE correlations of H-33 (δH 4.86, t, J = 6.4 Hz) with both H3-37 (δH 1.86, s) and H3-40 (δH 1.16, s). Moreover, the 13C NMR signals of C-37 (δC 19.3, CH3) and C-38 (δC 17.9, CH3) indicated the E geometry of the trisubstituted C-22/C-23 and tetrasubstituted C-34/C-35 double bonds. According to the above described evidence, and since compounds of this type are likely biosynthesized from the same or similar precursors and pathway, the absolute configuration of 1 was proposed as 1S,2S,9R,12S,21S,26S,27R,30R,31S,33S.

Figure 3

Key NOE correlations of compound 1.

The pseudomolecular ion peak of sardigitolide B (2) at m/z = 721.4283 indicated the same molecular formula of 2, C41H62O9, as that of 1. The 13C NMR spectrum of 2 showed similar signals to those of 1. Also, it displayed carbon signals of 9 methyls, 11 methylenes, 10 methines, and 11 quaternary carbons. By detailed analysis of the 2D NMR spectral data of 2, a similar gross structure to that of 1 was deduced. However, it was found that 2 possesses a remarkably upfield-shifted signal of H-4 (δH 5.85, s), while 1 displayed a downfield-shifted resonance for H-4 (δH 6.44, s). Also, the 13C NMR spectroscopy data were found to be quite similar to those of 1, with the exception of CH3-19 at δC 26.6 in 1 and δC 20.3 in 2, suggesting the presence of a trans geometry of 4,5-double bond.

The relative configurations of the stereogenic centers in 2 were determined from the NOESY spectrum, which also showed similar NOE correlations to those of 1. In contrast, H-4 (δH 5.85, s) showed an NOE correlation with H-2 (δH 3.73, m), but not with H3-19 (δH 2.12, s), confirming the E geometry of the double bond at C-4 and C-5 again. These results determined 2 to be the 4E isomer of 1.

Sardigitolide C (3) exhibited a protonated molecular peak at m/z 721.4281 in the HR-ESI-MS spectrum, establishing the same molecular formula, C41H62O9, and 11 degrees of unsaturation as those of 1 and 2. The 13C NMR spectroscopy data also showed 41 carbon signals, including 8 methyl groups, 13 methylene, 9 methines, and 11 quaternary carbons. Analyzing the 2D NMR spectral data of 3 in detail showed a similar gross structure of 3 to that of 1, except that the chemical shifts of CH2-19 (δH 4.72, 4.88, brs; δC 114.9) in 3 indicated the presence of 1,1-disubstituted double bond at C-5/C-19. The relative configuration of 3 was also determined from NOESY spectrum, which showed similar NOE correlations among the corresponding protons to those of 1. This is the first ximaolide-type compound containing a disubstituted double bond at C-5.

Sardigitolide D (4) was isolated as a white amorphous solid. The HR-ESI-MS of 4 showed a pseudomolecular ion peak at 721.4268 , indicating the molecular formula C41H62O9 and implying 11 degrees of unsaturation. In the 13C and DEPT-135 spectra, 4 showed 41 carbon signals resembling those of 1 including 9 methyls, 11 methylenes, 10 methines, and 11 quaternary carbons. Also, 4 had the same gross structure as 1 according to the detailed analyses of 1D and 2D NMR (COSY and HMBC) spectra. However, it was found that H-33 of 4 showed a different coupling constant (J = 10.5 Hz) to that of H-33 (J = 6.4 Hz) in 1. Furthermore, 4 did not show NOE correlation between H-22 (δH 4.99, d, J = 10.5 Hz) and H-33 (δH 4.78, d, J = 10.5 Hz), while 1–3 exhibited NOE interactions between H-22 and H-33, revealing the 33R configuration. On the basis of the above findings, the molecular structure of 4 was determined unambiguously to be the 33-epimer of 1.

It is known that IL-1β, the cytokine family member of interleukin-1 produced by activated macrophages, can lead to a strong immune response, and many studies have shown that IL-1β is related to various diseases, such as rheumatoid arthritis [31,32], neuropathic pain [33], and cardiovascular disease [34]. Furthermore, IL-1β has been suggested as a therapeutic target for the treatment of diabetic retinopathy [35,36]. In this study, we also attempted to screen the bioactivity of isolates with potential anti-inflammatory and cytotoxic abilities. Therefore, 1–8 were screened for their inhibitory potential against the upregulation of proinflammatory cytokine IL-1β in LPS-stimulated murine macrophage J774A.1 cells. The results showed that, at a concentration of 10 μg/mL, 6 and 8 potently inhibited LPS-induced IL-1β production to 68 ± 1 and 56 ± 1%, respectively, with IC50 values of 10.7 ± 2.7 and 14.9 ± 5.1 μg/mL, respectively. In addition, compounds 1–8 were assayed for their cytotoxicities toward MCF-7, MDA-MB-231, HepG2, and HeLa carcinoma cell lines. The results (Table 3) showed that sardigitolide B (2) exhibited inhibitory activity toward MCF-7and MDA-MB-231 cells with IC50 values of 9.6 ± 3.0 and 14.8 ± 4.0 μg/mL, respectively, and glacumolide A (6) showed cytotoxicity toward MCF-7, HepG2, and HeLa cell lines with IC50 values of 10.1 ± 3.3, 14.9 ± 3.5, and 17.1 ± 4.5 µg/mL, respectively. Glacumolide B (7) also was found to display cytotoxicity toward MCF-7, MDA-MB-231, and HepG2 cell lines, with IC50 values of 9.4 ± 3.0, 17.8 ± 4.5, and 14.9 ± 4.2 µg/mL, respectively. The monocembranoid 8 (molecular weight 374) showed cytotoxicity against the growth of MCF-7 cancer cell line with IC50 value of 10.9 ± 4.3 µg/mL (29.1 ± 11.4 µM). The remaining compounds did not show any effective cytotoxic and anti-inflammatory activities.

Table 3

Cytotoxicities of compounds 1–8. IC50 (µg/mL) values are the mean ± SEM (n = 3).

Compound	MCF-7	MDA-MB-231	HepG2	HeLa
1	– a	–	–	–
2	9.6 ± 3.0	14.8 ± 4.0	–	–
3	–	–	–	–
4	–	–	–	–
5	–	–	–	–
6	10.1 ± 3.3	–	14.9 ± 3.5	17.1 ± 4.5
7	9.4 ± 3.0	17.8 ± 4.5	14.9 ± 4.2	–
8	10.9 ± 4.3	–	–	–
Doxorubicin	0.7 ± 0.1	1.3 ± 0.2	1.2 ± 0.4	0.4 ± 0.1

a—> 20 µg/mL.

Methyl tortuosoate, also called methyl tetrahydrosarcoate (10) (Figure 4), has been frequently discovered to be the dienophile monomeric cembranoid in the Diels–Alder reaction to biosynthesize relevant biscembranoid, while methyl sarcoate (11) has been found to be the dienophile part of this series biscembranoids for only one time [37]. Biscembranoids 1 and 4 are the Diels–Alder reaction products using (7Z,8)-dehydromethyl tortuosoate (12), 2 using (7E,8)-dehydromethyl tortuosoate (13), and 3 using (7,19)-dehydromethyl tortuosoate (14), respectively, as the dienophiles in the biscembranoids biosynthesis. Cembranoids 12–14 are waiting for discovery in future investigations.

Figure 4

Structures of dienophiles 10–14.

Our previous investigation of secondary metabolites from the soft coral Sarcophyton glacum led to the discovery of two biscembranoid-type compounds, glaucumolide A (6) and glaucumolide B (7) [19]. The absolute configurations of 6, 7, and analogs have been confirmed in 2019 by Wen et al. from the X-ray single crystal diffraction of the same compound 6, isolated from the soft coral S. trocheliophorum [20]. Recently, with the development of molecular phylogenetic analysis technology, McFadden et al. suggested that S. glacum contained more than seven genetically distinct clades which might be the reason for the continuous discovery of high chemical diversity of secondary metabolites of the soft coral S. glacum [38]. For the same reason, it is possible that the corals of Sarcophyton genus could generate similar structure biscembranoids as our present study.

3. Materials and Methods

3.1. General Experimental Procedures

Optical rotations and IR spectra were recorded on a JASCO P-1020 polarimeter and FR/IR-4100 infrared spectrophotometer (Jasco Corporation, Tokyo, Japan), respectively. NMR spectra were acquired on a Varian Unity Inova 500 FT-NMR (Varian Inc., Palo Alto, CA, USA) at 500 and 125 MHz for 1H and 13C, respectively; or on Varian 400 MR FT-NMR instrument at 400 MHz and 100 MHz for 1H and 13C, respectively. The data of low-resolution electron spray ionization mass spectroscopy (LRESIMS) were measured by Bruker APEX II mass spectrometer. The data of HRESIMS were obtained by Bruker Apex-Qe 9.4T mass spectrometer. Silica gel 60 and reverse-phased silica gel (RP-18; 230–400 mesh) were used for normal-phase and reverse-phased column chromatography. Precoated silica gel plates (Kieselgel 60 F254, 0.2 mm) (Merck, Darmstadt, Germany) were used for analytical TLC. High-performance liquid chromatography was performed on a Hitachi L-2455 HPLC apparatus with a Supelco C18 column (ODS-3, 5 μm, 250 × 20 mm; Sciences Inc., Tokyo, Japan).

3.2. Soft Coral Material

The cultured soft coral Sarcophyton digitatum of this study was originally collected from the wild in an 80-ton cultivation tank (height 1.6 m) located in the National Museum of Marine Biology and Aquarium, Taiwan. The tank was designed to simulate the fringing reefs in southern Taiwan [39]. The bottom of the tank was covered by a 3–5 cm layer of coral sand, and artificial rocks were distributed on the sand. Sand-filtered seawater pumped directly from the adjacent reef coast and added to the tank at an exchange rate of 10% per day of the total volume. The tank was a semiclosed recirculating aquaculture system and did not require deliberate feeding [40]. The water temperatures were maintained between 24 and 27 °C via a heat-exchanger cooling system. The soft coral S. digitatum was harvested in the National Museum of Marine Biology & Aquarium, Pingtung, in 2014. The coral was identified by one of the authors of this study (C.-F.D.). The animal sample was stored at the Department of Marine Biotechnology and Marine Resources, National Sun Yat-Sen University.

3.3. Extraction and Isolation

A sample of S. digitatum (0.9 kg) was sliced and exhaustively extracted with ethyl acetate (1 L × 5), and the solvent-free residue was sequentially partitioned between water and n-hexane, ethyl acetate, and n-butanol to yield three samples from the above solvents. The resulted EtOAc extract (1.098 g) was purified over silica gel by column chromatography and eluted with acetone in n-hexane (10–100%, stepwise), and then with methanol in acetone (0–100%, stepwise) to yield 13 fractions. Fraction 2 was eluted with the solvent system of acetone–n-hexane (6:1) to obtain 4 subfractions (A–D). Subfraction 2-B was purified by RP-HPLC using MeOH/H2O (3:1) at a flow rate of 5 mL/min to afford 8 (2.1 mg). Fraction 3 was eluted with acetone–n-hexane (1:5) followed by isolation over a silica gel column to yield 5 subfractions (A–E). Subfraction 3-D was also purified by RP-HPLC with the solvent system of MeOH/H2O (4:1) at a flow rate of 5 mL/min to obtain 9 (3.4 mg). Fraction 10 was eluted with acetone–n-hexane (1:3) and rechromatographed over RP-18 gel to obtain 6 subfractions (A–F). Subfraction 10-F was exhaustively purified by RP-HPLC using MeOH/H2O (2:1) at a flow rate of 5 mL/min to yield 6 (20.2 mg) and 7 (13.5 mg). Fraction 11, eluting with acetone–n-hexane (1:2), was carefully isolated by a reverse-phase silica gel column to yield 6 subfractions (A−F). Subfraction 11-C was further purified by RP-HPLC using MeOH/H2O (2:1) at a flow rate of 5 mL/min to obtain 1 (8.6 mg), 2 (4.0 mg), 3 (2.5 mg), 4 (2.0 mg), and 5 (23.3 mg).

Compound 1: white powder; = +48 (c 1.3, CHCl3), IR (KBr) vmax 3433, 2958, 1705, 1603, 1436, 1373, 1205, 1135, 1061, 1029 cm−1, 13C and 1H NMR data, Table 1 and Table 2; ESIMS m/z 721 ; HRESIMS m/z 721.4287 (calcd for C41H62O9Na, 721.4292)

Compound 2: white powder; = +88 (c 0.1, CHCl3), IR (KBr) vmax 3398, 2927, 1704, 1607, 1372, 1204, 1135, 1070 cm−1, 13C and 1H NMR data, Table 1 and Table 2; ESIMS m/z 721 ; HRESIMS m/z 721.4283 (calcd for C41H62O9Na, 721.4292)

Compound 3: white powder; = +39 (c 0.8, CHCl3), IR (KBr) vmax 3445, 2959, 1703, 1435, 1372, 1206, 1061 cm−1, 13C and 1H NMR data, Table 1 and Table 2; ESIMS m/z 721 ; HRESIMS m/z 721.4281 (calcd for C41H62O9Na, 721.4292)

Compound 4: white powder; = +76 (c 0.5, CHCl3), IR (KBr) vmax 3446, 2957, 2925, 1706, 1603, 1435, 1375, 1206, 1061 cm−1, 13C and 1H NMR data, Table 1 and Table 2; ESIMS m/z 721 ; HRESIMS m/z 721.4268 (calcd for C41H62O9Na, 721.4292)

3.4. Cytotoxicity Assay

Cell lines were purchased from the American Type Culture Collection (Rockville, MD). Cytotoxicity assays were carried out using the MTT assays [41]. MCF-7, MDA-MB-231, and HepG2, and HeLa cell lines were cultured and then exposed to various concentrations of 1–8 in dimethyl sulfoxide (DMSO) for 72 h to screen cytotoxicity. Doxorubicin, the positive control of the MTT assay, showed cytotoxicity towards MCF-7, MDA-MB-231, HepG2, and HeLa cancer cell lines, with IC50 values of, 0.7 ± 0.1, 1.3 ± 0.2, 1.2 ± 0.4, and 0.4 ± 0.1 (µg/mL), respectively.

3.5. Anti-Inflammatory Assay

Murine macrophage cell line J774A.1 was purchased from the American Type Culture Collection (Rockville, MD). J774A.1 cells were cultured in 96-well plates at a concentration of 5 × 104 cells/mL and allowed to grow overnight. The cell cultures were added to the DMSO as control or different concentrations of compounds 1–8, followed by treatment with LPS 1 µg/mL for 24 h. Determination of the cytokine concentration was performed by ELISA according to the manufacturer’s protocol and previously reported method [42].

4. Conclusions

Investigation of Sarcophyton digitatum led to the purification of four new biscembranoids 1–4, along with five known compounds 5–9. With regards to biological activities, compounds 2, 6, and 7 displayed inhibitory activities against the proliferation of a limited panel of cancer cell lines in a cytotoxic assay. Furthermore, compounds 6 and 8 showed anti-inflammatory activity, which inhibited LPS-induced enhanced IL-1β production. The soft coral Sarcophyton sp. has been shown to produce various biscembranoid metabolites. Up to date, more than 60 biscembranoids biosynthesized via Diels–Alder reaction were isolated. Interestingly, these biscembranoids and their biosynthetic monocembranoid precursors are often found in Sarcophyton species [17,18,19,20,21,30,43,44,45,46]. The results of this study suggest that aquaculture of Sarcophyton species might produce structurally diversified bioactive compounds that could be beneficial for future drug development. Furthermore, the three dienophiles 12–14 are waiting for discovery as new cembranoids in future studies.