Literature DB >> 2821492

Cleavage of methylated CCCGGG sequences containing either N4-methylcytosine or 5-methylcytosine with MspI, HpaII, SmaI, XmaI and Cfr9I restriction endonucleases.

V Butkus1, L Petrauskiene, Z Maneliene, S Klimasauskas, V Laucys, A Janulaitis.   

Abstract

The cleavage specificity of R.Cfr9I was determined to be C decreases CCGGG whereas the methylation specificity of M.Cfr9I was C4mCCGGG. The action of MspI, HpaII, SmaI, XmaI and Cfr9I restriction endonucleases on an unmethylated parent d(GGACCCGGGTCC) dodecanucleotide duplex and a set of oligonucleotide duplexes, containing all possible substitutions of either 4mC or 5mC for C in the CCCGGG sequence, was investigated. It was found that 4mC methylation, in contrast to 5mC, renders the CCCGGG site resistant to practically all the investigated endonucleases. The cleavage of methylated substrates with restriction endonucleases is discussed.

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Year:  1987        PMID： 2821492      PMCID： PMC306195          DOI： 10.1093/nar/15.17.7091

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  17 in total

1.  A third restriction endonuclease from Xanthomonas malvacearum.

Authors:  L M Kunkel; M Silberklang; B J McCarthy
Journal:  J Mol Biol       Date:  1979-07-25       Impact factor: 5.469

2.  N4-methylcytosine as a minor base in bacterial DNA.

Authors:  M Ehrlich; G G Wilson; K C Kuo; C W Gehrke
Journal:  J Bacteriol       Date:  1987-03       Impact factor: 3.490

3.  DNA adenine methylation of GATC sequences appeared recently in the Escherichia coli lineage.

Authors:  T Barbeyron; K Kean; P Forterre
Journal:  J Bacteriol       Date:  1984-11       Impact factor: 3.490

4.  The sequence GGCmCGG is resistant to MspI cleavage.

Authors:  M Busslinger; E deBoer; S Wright; F G Grosveld; R A Flavell
Journal:  Nucleic Acids Res       Date:  1983-06-11       Impact factor: 16.971

5.  Cloning of the MspI modification enzyme. The site of modification and its effects on cleavage by MspI and HpaII.

Authors:  R Y Walder; C J Langtimm; R Chatterjee; J A Walder
Journal:  J Biol Chem       Date:  1983-01-25       Impact factor: 5.157

Review 6.  DNA methyltransferases of Bacillus subtilis and its bacteriophages.

Authors:  U Günthert; T A Trautner
Journal:  Curr Top Microbiol Immunol       Date:  1984       Impact factor: 4.291

7.  Cytosine modification in DNA by BcnI methylase yields N4-methylcytosine.

Authors:  A Janulaitis; S Klimasauskas; M Petrusyte; V Butkus
Journal:  FEBS Lett       Date:  1983-09-05       Impact factor: 4.124

8.  DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors:  E Jay; R Bambara; R Padmanabhan; R Wu
Journal:  Nucleic Acids Res       Date:  1974-03       Impact factor: 16.971

9.  Methylated and unmethylated DNA compartments in the sea urchin genome.

Authors:  A P Bird; M H Taggart; B A Smith
Journal:  Cell       Date:  1979-08       Impact factor: 41.582

10.  Quantitative reversed-phase high-performance liquid chromatography of major and modified nucleosides in DNA.

Authors:  C W Gehrke; R A McCune; M A Gama-Sosa; M Ehrlich; K C Kuo
Journal:  J Chromatogr       Date:  1984-09-28
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  33 in total

1.  Recognition of native DNA methylation by the PvuII restriction endonuclease.

Authors:  M R Rice; R M Blumenthal
Journal:  Nucleic Acids Res       Date:  2000-08-15       Impact factor: 16.971

2.  Circular permutation of DNA cytosine-N4 methyltransferases: in vivo coexistence in the BcnI system and in vitro probing by hybrid formation.

Authors:  Giedrius Vilkaitis; Arvydas Lubys; Egle Merkiene; Albertas Timinskas; Arvydas Janulaitis; Saulius Klimasauskas
Journal:  Nucleic Acids Res       Date:  2002-04-01       Impact factor: 16.971

3.  Crystal structure of the Bse634I restriction endonuclease: comparison of two enzymes recognizing the same DNA sequence.

Authors:  Saulius Grazulis; Markus Deibert; Renata Rimseliene; Remigijus Skirgaila; Giedrius Sasnauskas; Arunas Lagunavicius; Vladimir Repin; Claus Urbanke; Robert Huber; Virginijus Siksnys
Journal:  Nucleic Acids Res       Date:  2002-02-15       Impact factor: 16.971

4.  Alw26I, Eco31I and Esp3I--type IIs methyltransferases modifying cytosine and adenine in complementary strands of the target DNA.

Authors:  J Bitinaite; Z Maneliene; S Menkevicius; S Klimasauskas; V Butkus; A Janulaitis
Journal:  Nucleic Acids Res       Date:  1992-10-11       Impact factor: 16.971

5.  Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors:  M McClelland; M Nelson
Journal:  Nucleic Acids Res       Date:  1992-05-11       Impact factor: 16.971

6.  Sequence-specific DNA binding by the MspI DNA methyltransferase.

Authors:  A K Dubey; R J Roberts
Journal:  Nucleic Acids Res       Date:  1992-06-25       Impact factor: 16.971

7.  Structure and evolution of the XcyI restriction-modification system.

Authors:  B E Withers; L A Ambroso; J C Dunbar
Journal:  Nucleic Acids Res       Date:  1992-12-11       Impact factor: 16.971

8.  M.Smal is an N4-methylcytosine specific DNA-methylase.

Authors:  S Klimasauskas; D Steponaviciene; Z Maneliene; M Petrusyte; V Butkus; A Janulaitis
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

9.  Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.

Authors:  M Nelson; M McClelland
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

10.  Determination of methylation specificity of DsaV methyltransferase by a simple biochemical method.

Authors:  J Gopal; A S Bhagwat
Journal:  Nucleic Acids Res       Date:  1995-01-11       Impact factor: 16.971
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