Literature DB >> 29751015

Effect of tRNA on the Maturation of HIV-1 Reverse Transcriptase.

Tatiana V Ilina1, Ryan L Slack2, John H Elder3, Stefan G Sarafianos4, Michael A Parniak5, Rieko Ishima6.   

Abstract

The mature HIV-1 reverse transcriptase is a heterodimer that comprises 66 kDa (p66) and 51 kDa (p51) subunits. The latter is formed by HIV-1 protease-catalyzed removal of a C-terminal ribonuclease H domain from a p66 subunit. This proteolytic processing is a critical step in virus maturation and essential for viral infectivity. Here, we report that tRNA significantly enhances in vitro processing even at a substoichiometric tRNA:p66/p66 ratio. Other double-stranded RNAs have considerably less pronounced effect. Our data support a model where interaction of p66/p66 with tRNA introduces conformational asymmetry in the two subunits, permitting specific proteolytic processing of one p66 to provide the mature RT p66/p51 heterodimer.
Copyright © 2018 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  HIV-1; RNase H; maturation; proteolysis; reverse transcriptase; tRNA

Mesh：

Substances：

Year:  2018        PMID： 29751015      PMCID： PMC5988984          DOI： 10.1016/j.jmb.2018.02.027

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  49 in total

1.  Factors affecting the dimerization of the p66 form of HIV-1 reverse transcriptase.

Authors:  J F Cabodevilla; L Odriozola; E Santiago; J J Martínez-Irujo
Journal:  Eur J Biochem       Date:  2001-03

2.  Profiling non-lysyl tRNAs in HIV-1.

Authors:  Mariana Pavon-Eternod; Min Wei; Tao Pan; Lawrence Kleiman
Journal:  RNA       Date:  2009-12-09       Impact factor: 4.942

Review 3.  Understanding nucleic acid-ion interactions.

Authors:  Jan Lipfert; Sebastian Doniach; Rhiju Das; Daniel Herschlag
Journal:  Annu Rev Biochem       Date:  2014-03-05       Impact factor: 23.643

4.  Interaction of HIV-1 reverse transcriptase ribonuclease H with an acylhydrazone inhibitor.

Authors:  Qingguo Gong; Lakshmi Menon; Tatiana Ilina; Lena G Miller; Jinwoo Ahn; Michael A Parniak; Rieko Ishima
Journal:  Chem Biol Drug Des       Date:  2010-11-29       Impact factor: 2.817

5.  Alterations to the primer grip of p66 HIV-1 reverse transcriptase and their consequences for template-primer utilization.

Authors:  M Ghosh; P S Jacques; D W Rodgers; M Ottman; J L Darlix; S F Le Grice
Journal:  Biochemistry       Date:  1996-07-02       Impact factor: 3.162

6.  Analysis of the S3 and S3' subsite specificities of feline immunodeficiency virus (FIV) protease: development of a broad-based protease inhibitor efficacious against FIV, SIV, and HIV in vitro and ex vivo.

Authors:  T Lee; G S Laco; B E Torbett; H S Fox; D L Lerner; J H Elder; C H Wong
Journal:  Proc Natl Acad Sci U S A       Date:  1998-02-03       Impact factor: 11.205

7.  The p66 immature precursor of HIV-1 reverse transcriptase.

Authors:  Naima G Sharaf; Eric Poliner; Ryan L Slack; Martin T Christen; In-Ja L Byeon; Michael A Parniak; Angela M Gronenborn; Rieko Ishima
Journal:  Proteins       Date:  2014-05-12

8.  Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA.

Authors:  A Jacobo-Molina; J Ding; R G Nanni; A D Clark; X Lu; C Tantillo; R L Williams; G Kamer; A L Ferris; P Clark
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-01       Impact factor: 11.205

9.  In vivo processing of Pr160gag-pol from human immunodeficiency virus type 1 (HIV) in acutely infected, cultured human T-lymphocytes.

Authors:  H Lindhofer; K von der Helm; H Nitschko
Journal:  Virology       Date:  1995-12-20       Impact factor: 3.616

10.  Selective unfolding of one Ribonuclease H domain of HIV reverse transcriptase is linked to homodimer formation.

Authors:  Xunhai Zheng; Lars C Pedersen; Scott A Gabel; Geoffrey A Mueller; Matthew J Cuneo; Eugene F DeRose; Juno M Krahn; Robert E London
Journal:  Nucleic Acids Res       Date:  2014-02-25       Impact factor: 16.971
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  7 in total

Review 1.  HIV-1 Reverse Transcriptase: A Metamorphic Protein with Three Stable States.

Authors:  Robert E London
Journal:  Structure       Date:  2019-01-10       Impact factor: 5.006

2.  Conformational Changes in HIV-1 Reverse Transcriptase that Facilitate Its Maturation.

Authors:  Ryan L Slack; Tatiana V Ilina; Zhaoyong Xi; Nicholas S Giacobbi; Gota Kawai; Michael A Parniak; Stefan G Sarafianos; Nicolas Sluis Cremer; Rieko Ishima
Journal:  Structure       Date:  2019-08-27       Impact factor: 5.006

Review 3.  Evolving understanding of HIV-1 reverse transcriptase structure, function, inhibition, and resistance.

Authors:  Francesc Xavier Ruiz; Eddy Arnold
Journal:  Curr Opin Struct Biol       Date:  2020-01-11       Impact factor: 6.809

4.  Effect of Lysyl-tRNA Synthetase on the Maturation of HIV-1 Reverse Transcriptase.

Authors:  Tatiana V Ilina; Ryan L Slack; Michel Guerrero; Rieko Ishima
Journal:  ACS Omega       Date:  2020-06-30

Review 5.  Large Multidomain Protein NMR: HIV-1 Reverse Transcriptase Precursor in Solution.

Authors:  Tatiana V Ilina; Zhaoyong Xi; Teresa Brosenitsch; Nicolas Sluis-Cremer; Rieko Ishima
Journal:  Int J Mol Sci       Date:  2020-12-15       Impact factor: 5.923

6.  Binding interface and impact on protease cleavage for an RNA aptamer to HIV-1 reverse transcriptase.

Authors:  Phuong D M Nguyen; Jie Zheng; Thomas J Gremminger; Liming Qiu; Dong Zhang; Steve Tuske; Margaret J Lange; Patrick R Griffin; Eddy Arnold; Shi-Jie Chen; Xiaoqin Zou; Xiao Heng; Donald H Burke
Journal:  Nucleic Acids Res       Date:  2020-03-18       Impact factor: 16.971

Review 7.  Interplay between Host tRNAs and HIV-1: A Structural Perspective.

Authors:  Jinwei Zhang
Journal:  Viruses       Date:  2021-09-13       Impact factor: 5.048
  7 in total

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