| Literature DB >> 29406750 |
Pengfei Wang1, Mohammad Aminur Rahman2, Zhixiang Zhao2,3, Kristin Weiss1, Chao Zhang4, Zhengjia Chen4, Selwyn J Hurwitz5, Zhuo G Chen2, Dong M Shin1,2, Yonggang Ke1,6.
Abstract
DNA origami is a promising molecular delivery system for a variety of therapeutic applications including cancer therapy, given its capability to fabricate homogeneous nanostructures whose physicochemical properties (size, shape, surface chemistry) can be precisely tailored. However, the correlation between DNA-origami design and internalization efficiency in different cancer cell lines remains elusive. We investigated the cellular uptake of four DNA-origami nanostructures (DONs) with programmed sizes and shapes in multiple human cancer cell lines. The cellular uptake efficiency of DONs was influenced by size, shape, and cell line. Scavenger receptors were responsible for the internalization of DONs into cancer cells. We observed distinct stages of the internalization process of a gold nanoparticle (AuNP)-tagged rod-shape DON, using high-resolution transmission electron microscopy. This study provides detailed understanding of cellular uptake and intracellular trafficking of DONs in cancer cells, and offers new insights for future optimization of DON-based drug delivery systems for cancer treatment.Entities:
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Year: 2018 PMID： 29406750 PMCID： PMC7261494 DOI： 10.1021/jacs.7b09024
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419