Literature DB >> 29143058

Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome b5.

Thirupathi Ravula1, Carlo Barnaba, Mukesh Mahajan, G M Anantharamaiah, Sang-Choul Im, Lucy Waskell, Ayyalusamy Ramamoorthy.   

Abstract

Heme's spin-multiplicity is key in determining the enzymatic function of cytochrome P450 (cytP450). The origin of the low-spin state in ferric P450 is still under debate. Here, we report the first experimental demonstration of P450's membrane interaction altering its spin equilibrium which is accompanied by a stronger affinity for cytochrome b5. These results highlight the importance of lipid membrane for the function of P450.

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Year:  2017        PMID： 29143058      PMCID： PMC5710748          DOI： 10.1039/c7cc07520k

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  22 in total

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Review 2.  Structure and chemistry of cytochrome P450.

Authors:  Ilia G Denisov; Thomas M Makris; Stephen G Sligar; Ilme Schlichting
Journal:  Chem Rev       Date:  2005-06       Impact factor: 60.622

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Journal:  Biochem Soc Trans       Date:  1979-12       Impact factor: 5.407

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Journal:  FEBS Lett       Date:  1977       Impact factor: 4.124

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Journal:  Biochem J       Date:  1996-11-01       Impact factor: 3.857

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Authors:  F Peter Guengerich; Martha V Martin; Christal D Sohl; Qian Cheng
Journal:  Nat Protoc       Date:  2009-08-06       Impact factor: 13.491

7.  The orientations of cytochrome c in the highly dynamic complex with cytochrome b5 visualized by NMR and docking using HADDOCK.

Authors:  Alexander N Volkov; Davide Ferrari; Jonathan A R Worrall; Alexandre M J J Bonvin; Marcellus Ubbink
Journal:  Protein Sci       Date:  2005-02-02       Impact factor: 6.725

8.  Membrane Fluidity Modulates Thermal Stability and Ligand Binding of Cytochrome P4503A4 in Lipid Nanodiscs.

Authors:  Wynton D McClary; John P Sumida; Michele Scian; Lorela Paço; William M Atkins
Journal:  Biochemistry       Date:  2016-11-01       Impact factor: 3.162

9.  Reconstitution premixes for assays using purified recombinant human cytochrome P450, NADPH-cytochrome P450 reductase, and cytochrome b5.

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Journal:  Arch Biochem Biophys       Date:  1997-12-01       Impact factor: 4.013

Review 10.  The catalytic function of cytochrome P450 is entwined with its membrane-bound nature.

Authors:  Carlo Barnaba; Katherine Gentry; Nirupama Sumangala; Ayyalusamy Ramamoorthy
Journal:  F1000Res       Date:  2017-05-09
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  18 in total

1.  Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs.

Authors:  Thirupathi Ravula; Nathaniel Z Hardin; Jia Bai; Sang-Choul Im; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  Chem Commun (Camb)       Date:  2018-08-23       Impact factor: 6.222

2.  Probing protein-protein and protein-substrate interactions in the dynamic membrane-associated ternary complex of cytochromes P450, b5, and reductase.

Authors:  Katherine A Gentry; G M Anantharamaiah; Ayyalusamy Ramamoorthy
Journal:  Chem Commun (Camb)       Date:  2019-10-22       Impact factor: 6.222

3.  Supramolecular Organization of Apolipoprotein-A-I-Derived Peptides within Disc-like Arrangements.

Authors:  Evgeniy S Salnikov; G M Anantharamaiah; Burkhard Bechinger
Journal:  Biophys J       Date:  2018-07-11       Impact factor: 4.033

4.  Nanodisc-Forming Scaffold Protein Promoted Retardation of Amyloid-Beta Aggregation.

Authors:  Bikash Ranjan Sahoo; Takuya Genjo; Sarah J Cox; Andrea K Stoddard; G M Anantharamaiah; Carol Fierke; Ayyalusamy Ramamoorthy
Journal:  J Mol Biol       Date:  2018-08-28       Impact factor: 5.469

5.  A Minimal Functional Complex of Cytochrome P450 and FBD of Cytochrome P450 Reductase in Nanodiscs.

Authors:  Elke Prade; Mukesh Mahajan; Sang-Choul Im; Meng Zhang; Katherine A Gentry; G M Anantharamaiah; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  Angew Chem Int Ed Engl       Date:  2018-06-14       Impact factor: 15.336

6.  Cytochrome-P450-Induced Ordering of Microsomal Membranes Modulates Affinity for Drugs.

Authors:  Carlo Barnaba; Bikash Ranjan Sahoo; Thirupathi Ravula; Ilce G Medina-Meza; Sang-Choul Im; G M Anantharamaiah; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  Angew Chem Int Ed Engl       Date:  2018-02-22       Impact factor: 15.336

7.  Two Distinct Structures of Membrane-Associated Homodimers of GTP- and GDP-Bound KRAS4B Revealed by Paramagnetic Relaxation Enhancement.

Authors:  Ki-Young Lee; Zhenhao Fang; Masahiro Enomoto; Genevieve Gasmi-Seabrook; Le Zheng; Shohei Koide; Mitsuhiko Ikura; Christopher B Marshall
Journal:  Angew Chem Int Ed Engl       Date:  2020-04-30       Impact factor: 15.336

8.  Magnetic Alignment of Polymer Nanodiscs Probed by Solid-State NMR Spectroscopy.

Authors:  Thirupathi Ravula; JaeWoong Kim; Dong-Kuk Lee; Ayyalusamy Ramamoorthy
Journal:  Langmuir       Date:  2020-01-30       Impact factor: 3.882

9.  Conformational turn triggers regio-selectivity in the bioactivation of thiophene-contained compounds mediated by cytochrome P450.

Authors:  Chun-Zhi Ai; Yong Liu; Du-Chu Chen; Yasmeen Saeed; Yi-Zhou Jiang
Journal:  J Biol Inorg Chem       Date:  2019-09-10       Impact factor: 3.358

10.  Expression, purification, and functional reconstitution of 19F-labeled cytochrome b5 in peptide nanodiscs for NMR studies.

Authors:  Jia Bai; Jian Wang; Thirupathi Ravula; Sang-Choul Im; G M Anantharamaiah; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  Biochim Biophys Acta Biomembr       Date:  2020-01-15       Impact factor: 3.747
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