Literature DB >> 19661994

Measurement of cytochrome P450 and NADPH-cytochrome P450 reductase.

F Peter Guengerich1, Martha V Martin, Christal D Sohl, Qian Cheng.   

Abstract

Cytochrome P450 (P450) enzymes are important in the metabolism of steroids, vitamins, carcinogens, drugs and other compounds. Two of the commonly used assays in this field are the measurements of total P450 and NADPH-P450 reductase in biological preparations. A detailed protocol is presented for the measurement of P450 by its spectral properties, along with a protocol for measuring NADPH-P450 reductase by its NADPH-cytochrome c reduction activity. Each assay can be completed in 5-10 min. Detailed explanations for the rationale of particular sequences in the protocols are provided, along with potential confounding problems.

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Year:  2009        PMID： 19661994      PMCID： PMC3843963          DOI： 10.1038/nprot.2009.121

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  27 in total

1.  Quantitative determination of cytochrome P-450 in rat liver homogenate.

Authors:  T Matsubara; M Koike; A Touchi; Y Tochino; K Sugeno
Journal:  Anal Biochem       Date:  1976-10       Impact factor: 3.365

2.  Destruction of heme and hemoproteins mediated by liver microsomal reduced nicotinamide adenine dinucleotide phosphate-cytochrome P-450 reductase.

Authors:  F P Guengerich
Journal:  Biochemistry       Date:  1978-08-22       Impact factor: 3.162

3.  Identification of the high and low potential flavins of liver microsomal NADPH-cytochrome P-450 reductase.

Authors:  J L Vermilion; M J Coon
Journal:  J Biol Chem       Date:  1978-12-25       Impact factor: 5.157

4.  Microsomal electron transport: tetrazolium reduction by rat liver microsomal NADPH-cytochrome c reductase.

Authors:  D L Roerig; L Mascaro; S D Aust
Journal:  Arch Biochem Biophys       Date:  1972-12       Impact factor: 4.013

5.  Purification of cytochrome P-450, NADPH-cytochrome P-450 reductase, and epoxide hydratase from a single preparation of rat liver microsomes.

Authors:  F P Guengerich; M V Martin
Journal:  Arch Biochem Biophys       Date:  1980-12       Impact factor: 4.013

6.  Measurement of cytochrome P-450 in the presence of large amounts of contaminating hemoglobin and methemoglobin.

Authors:  K A Johannesen; J W DePierre
Journal:  Anal Biochem       Date:  1978-06-01       Impact factor: 3.365

7.  Some properties of a detergent-solubilized NADPH-cytochrome c(cytochrome P-450) reductase purified by biospecific affinity chromatography.

Authors:  Y Yasukochi; B S Masters
Journal:  J Biol Chem       Date:  1976-09-10       Impact factor: 5.157

Review 8.  Drug-drug interactions for UDP-glucuronosyltransferase substrates: a pharmacokinetic explanation for typically observed low exposure (AUCi/AUC) ratios.

Authors:  J Andrew Williams; Ruth Hyland; Barry C Jones; Dennis A Smith; Susan Hurst; Theunis C Goosen; Vincent Peterkin; Jeffrey R Koup; Simon E Ball
Journal:  Drug Metab Dispos       Date:  2004-08-10       Impact factor: 3.922

9.  Purified liver microsomal NADPH-cytochrome P-450 reductase. Spectral characterization of oxidation-reduction states.

Authors:  J L Vermilion; M J Coon
Journal:  J Biol Chem       Date:  1978-04-25       Impact factor: 5.157

10.  Novel properties of human placental aromatase as cytochrome P-450: purification and characterization of a unique form of aromatase.

Authors:  N Harada
Journal:  J Biochem       Date:  1988-01       Impact factor: 3.387
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  100 in total

1.  Metabolic capabilities of cytochrome P450 enzymes in Chinese liver microsomes compared with those in Caucasian liver microsomes.

Authors:  Junling Yang; Minxia M He; Wei Niu; Steven A Wrighton; Li Li; Yang Liu; Chuan Li
Journal:  Br J Clin Pharmacol       Date:  2012-02       Impact factor: 4.335

2.  Absolute Quantitation of Drug-Metabolizing Cytochrome P450 Enzymes and Accessory Proteins in Dog Liver Microsomes Using Label-Free Standard-Free Analysis Reveals Interbreed Variability.

Authors:  Stephanie E Martinez; Jian Shi; Hao-Jie Zhu; Tania E Perez Jimenez; Zhaohui Zhu; Michael H Court
Journal:  Drug Metab Dispos       Date:  2019-08-19       Impact factor: 3.922

3.  Human recombinant cytochrome P450 enzymes display distinct hydrogen peroxide generating activities during substrate independent NADPH oxidase reactions.

Authors:  Vladimir Mishin; Diane E Heck; Debra L Laskin; Jeffrey D Laskin
Journal:  Toxicol Sci       Date:  2014-07-24       Impact factor: 4.849

4.  Parameterization of Microsomal and Cytosolic Scaling Factors: Methodological and Biological Considerations for Scalar Derivation and Validation.

Authors:  Michael J Doerksen; Robert S Jones; Michael W H Coughtrie; Abby C Collier
Journal:  Eur J Drug Metab Pharmacokinet       Date:  2020-12-19       Impact factor: 2.441

Review 5.  Spectroscopic studies of the cytochrome P450 reaction mechanisms.

Authors:  Piotr J Mak; Ilia G Denisov
Journal:  Biochim Biophys Acta Proteins Proteom       Date:  2017-06-28       Impact factor: 3.036

6.  Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7.

Authors:  Yisheng Wu; Matthew L Hillwig; Qiang Wang; Reuben J Peters
Journal:  FEBS Lett       Date:  2011-10-05       Impact factor: 4.124

7.  Altered CYP2C9 activity following modulation of CYP3A4 levels in human hepatocytes: an example of protein-protein interactions.

Authors:  Diane Ramsden; Donald J Tweedie; Tom S Chan; Timothy S Tracy
Journal:  Drug Metab Dispos       Date:  2014-08-25       Impact factor: 3.922

8.  Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome b5.

Authors:  Thirupathi Ravula; Carlo Barnaba; Mukesh Mahajan; G M Anantharamaiah; Sang-Choul Im; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  Chem Commun (Camb)       Date:  2017-11-28       Impact factor: 6.222

9.  Carbon-carbon bond cleavage in activation of the prodrug nabumetone.

Authors:  Fatbardha Varfaj; Siti N A Zulkifli; Hyoung-Goo Park; Victoria L Challinor; James J De Voss; Paul R Ortiz de Montellano
Journal:  Drug Metab Dispos       Date:  2014-02-28       Impact factor: 3.922

10.  Zinc finger nuclease knock-out of NADPH:cytochrome P450 oxidoreductase (POR) in human tumor cell lines demonstrates that hypoxia-activated prodrugs differ in POR dependence.

Authors:  Jiechuang Su; Yongchuan Gu; Frederik B Pruijn; Jeff B Smaill; Adam V Patterson; Christopher P Guise; William R Wilson
Journal:  J Biol Chem       Date:  2013-11-06       Impact factor: 5.157
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