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Literature DB >> 2881276

Isolation of polymorphic DNA fragments from human chromosome 4.

T C Gilliam, S T Healey, M E MacDonald, G D Stewart, J J Wasmuth, R E Tanzi, J C Roy, J F Gusella.

Abstract

We have identified and characterized 40 DNA probes detecting restriction fragment length polymorphism (RFLP) on human chromosome 4. Single copy human clones were isolated from a bacteriophage library enriched for chromosome 4 sequences. Each clone was hybridized to somatic cell hybrid DNAs for verification of its species and chromosomal origin and for regional localization. Sequences specific for chromosome 4 were tested for their ability to detect RFLPs in human DNA and their potential utility as genetic markers was assessed. Approximately 263,000 base pairs or 0.13% of the chromosome was screened for sequence variation. The estimate of heterozygosity calculated from this large body of data, H = 0.0021, indicates that the degree of sequence variation on chromosome 4 is comparable to other autosomes. The characterization of these 40 markers has tripled the number of polymorphic loci available for linkage studies on chromosome 4, making it feasible to begin construction of a detailed linkage map that will span the entire chromosome.

Entities: Species

Mesh：

Substances：
DNA Restriction Enzymes

Year: 1987 PMID： 2881276 PMCID： PMC340560 DOI： 10.1093/nar/15.4.1445

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

30 in total

1. Precise localization of human beta-globin gene complex on chromosome 11.

Authors: J Gusella; A Varsanyi-Breiner; F T Kao; C Jones; T T Puck; C Keys; S Orkin; D Housman
Journal: Proc Natl Acad Sci U S A Date: 1979-10 Impact factor: 11.205

2. Polymorphic DNA region adjacent to the 5' end of the human insulin gene.

Authors: G I Bell; J H Karam; W J Rutter
Journal: Proc Natl Acad Sci U S A Date: 1981-09 Impact factor: 11.205

Review 3. DNA restriction fragment length polymorphisms and heterozygosity in the human genome.

Authors: D N Cooper; J Schmidtke
Journal: Hum Genet Date: 1984 Impact factor: 4.132

4. Linkage disequilibrium and evolutionary relationships of DNA variants (restriction enzyme fragment length polymorphisms) at the serum albumin locus.

Authors: J C Murray; K A Mills; C M Demopulos; S Hornung; A G Motulsky
Journal: Proc Natl Acad Sci U S A Date: 1984-06 Impact factor: 11.205

5. A polymorphic DNA marker genetically linked to Huntington's disease.

Authors: J F Gusella; N S Wexler; P M Conneally; S L Naylor; M A Anderson; R E Tanzi; P C Watkins; K Ottina; M R Wallace; A Y Sakaguchi
Journal: Nature Date: 1983 Nov 17-23 Impact factor: 49.962

6. A highly polymorphic locus in human DNA.

Authors: A R Wyman; R White
Journal: Proc Natl Acad Sci U S A Date: 1980-11 Impact factor: 11.205

7. Isolation and localization of DNA segments from specific human chromosomes.

Authors: J F Gusella; C Keys; A VarsanyiBreiner; F T Kao; C Jones; T T Puck; D Housman
Journal: Proc Natl Acad Sci U S A Date: 1980-05 Impact factor: 11.205

8. Restriction sites containing CpG show a higher frequency of polymorphism in human DNA.

Authors: D Barker; M Schafer; R White
Journal: Cell Date: 1984-01 Impact factor: 41.582

Review 9. Construction of a genetic linkage map in man using restriction fragment length polymorphisms.

Authors: D Botstein; R L White; M Skolnick; R W Davis
Journal: Am J Hum Genet Date: 1980-05 Impact factor: 11.025

10. A cell hybrid and recombinant DNA library that facilitate identification of polymorphic loci in the vicinity of the Huntington disease gene.

Authors: J J Wasmuth; L R Carlock; B Smith; L L Immken
Journal: Am J Hum Genet Date: 1986-09 Impact factor: 11.025

9 in total

1. Multiplex genotype determination at a large number of gene loci.

Authors: Z Lin; X Cui; H Li
Journal: Proc Natl Acad Sci U S A Date: 1996-03-19 Impact factor: 11.205

2. Chromosome jumping from D4S10 (G8) toward the Huntington disease gene.

Authors: J E Richards; T C Gilliam; J L Cole; M L Drumm; J J Wasmuth; J F Gusella; F S Collins
Journal: Proc Natl Acad Sci U S A Date: 1988-09 Impact factor: 11.205

3. Mapping of D4S98/S114/S113 confines the Huntington's defect to a reduced physical region at the telomere of chromosome 4.

Authors: W L Whaley; F Michiels; M E MacDonald; D Romano; M Zimmer; B Smith; J Leavitt; M Bucan; J L Haines; T C Gilliam
Journal: Nucleic Acids Res Date: 1988-12-23 Impact factor: 16.971

4. Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction.

Authors: J L Weber; P E May
Journal: Am J Hum Genet Date: 1989-03 Impact factor: 11.025

5. Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.

Authors: D Pinkel; J Landegent; C Collins; J Fuscoe; R Segraves; J Lucas; J Gray
Journal: Proc Natl Acad Sci U S A Date: 1988-12 Impact factor: 11.205

6. Isolation and regional localization of a large collection (2,000) of single-copy DNA fragments on human chromosome 3 for mapping and cloning tumor suppressor genes.

Authors: M I Lerman; F Latif; G M Glenn; L N Daniel; H Brauch; S Hosoe; K Hampsch; J Delisio; M L Orcutt; O W McBride
Journal: Hum Genet Date: 1991-04 Impact factor: 4.132