| Literature DB >> 28809392 |
Kai Xu1,2, Ying Yang3, Gui-Hai Feng1, Bao-Fa Sun3, Jun-Qing Chen1,4, Yu-Fei Li1,2, Yu-Sheng Chen3,2, Xin-Xin Zhang1,2, Chen-Xin Wang1,2, Li-Yuan Jiang1,2, Chao Liu1,2, Ze-Yu Zhang5,2, Xiu-Jie Wang5,2, Qi Zhou1,2, Yun-Gui Yang3,2, Wei Li1,2.
Abstract
METTL3 catalyzes the formation of N6-methyl-adenosine (m6A) which has important roles in regulating various biological processes. However, the in vivo function of Mettl3 remains largely unknown in mammals. Here we generated germ cell-specific Mettl3 knockout mice and demonstrated that Mettl3 was essential for male fertility and spermatogenesis. The ablation of Mettl3 in germ cells severely inhibited spermatogonial differentiation and blocked the initiation of meiosis. Transcriptome and m6A profiling analysis revealed that genes functioning in spermatogenesis had altered profiles of expression and alternative splicing. Our findings provide novel insights into the function and regulatory mechanisms of Mettl3-mediated m6A modification in spermatogenesis and reproduction in mammals.Entities:
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Year: 2017 PMID： 28809392 PMCID： PMC5587845 DOI： 10.1038/cr.2017.100
Source DB: PubMed Journal: Cell Res ISSN： 1001-0602 Impact factor: 25.617