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Literature DB >> 2859593

Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs.

Abstract

We have isolated and sequenced full-length cDNA clones encoding the human basally expressed H3.3 histone from a human fibroblast cDNA library. Several features of this atypical cDNA distinguish it and its gene from the well-characterized cell-cycle regulated histone genes and their RNA transcripts. The H3.3 mRNA is approximately equal to 1200 bases long, contains unusually long 5' and 3' untranslated regions, and has a 3' polyadenylylated terminus. In addition, we have isolated and characterized a cDNA clone that is a precursor to the H3.3 mRNA and contains an intervening sequence interrupting its 5' untranslated region. Hybridization of subsegments of the cDNA to human genomic DNA reveals a complex multigene family. The differences in the structures of basal and cell-cycle histone genes suggest a model to explain the differences in their expression.

Entities: Gene Species

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Year: 1985 PMID： 2859593 PMCID： PMC397660 DOI： 10.1073/pnas.82.9.2834

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

26 in total

1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors: M Grunstein; D S Hogness
Journal: Proc Natl Acad Sci U S A Date: 1975-10 Impact factor: 11.205

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

3. Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland.

Authors: O Hagenbüchle; R Bovey; R A Young
Journal: Cell Date: 1980-08 Impact factor: 41.582

4. Non-allelic variants of histones 2a, 2b and 3 in mammals.

Authors: S G Franklin; A Zweidler
Journal: Nature Date: 1977-03-17 Impact factor: 49.962

5. Separation of basal histone synthesis from S-phase histone synthesis in dividing cells.

Authors: R S Wu; W M Bonner
Journal: Cell Date: 1981-12 Impact factor: 41.582

6. Histone genes are clustered but not tandemly repeated in the chicken genome.

Authors: J D Engel; J B Dodgson
Journal: Proc Natl Acad Sci U S A Date: 1981-05 Impact factor: 11.205

7. Isolation of two clusters of mouse histone genes.

Authors: D B Sittman; I M Chiu; C J Pan; R H Cohn; L H Kedes; W F Marzluff
Journal: Proc Natl Acad Sci U S A Date: 1981-07 Impact factor: 11.205

8. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

9. Human spleen histone H3. Isolation and amino acid sequence.

Authors: Y Ohe; K Iwai
Journal: J Biochem Date: 1981-10 Impact factor: 3.387

10. The structure of the human histone genes: clustered but not tandemly repeated.

Authors: N Heintz; M Zernik; R G Roeder
Journal: Cell Date: 1981-06 Impact factor: 41.582

72 in total

1. Replication-dependent histone gene expression is related to Cajal body (CB) association but does not require sustained CB contact.

Authors: L S Shopland; M Byron; J L Stein; J B Lian; G S Stein; J B Lawrence
Journal: Mol Biol Cell Date: 2001-03 Impact factor: 4.138

Review 2. Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.

Authors: J Zhao; L Hyman; C Moore
Journal: Microbiol Mol Biol Rev Date: 1999-06 Impact factor: 11.056

10. Analysis of histone gene expression in adult tissues of the sea urchins Strongylocentrotus purpuratus and Lytechinus pictus: tissue-specific expression of sperm histone genes.

Authors: T Lieber; K Weisser; G Childs
Journal: Mol Cell Biol Date: 1986-07 Impact factor: 4.272

Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs.

1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

3. Tissue-specific expression of mouse-alpha-amylase genes: nucleotide sequence of isoenzyme mRNAs from pancreas and salivary gland.

4. Non-allelic variants of histones 2a, 2b and 3 in mammals.

5. Separation of basal histone synthesis from S-phase histone synthesis in dividing cells.

6. Histone genes are clustered but not tandemly repeated in the chicken genome.

7. Isolation of two clusters of mouse histone genes.

8. DNA sequencing with chain-terminating inhibitors.

9. Human spleen histone H3. Isolation and amino acid sequence.

10. The structure of the human histone genes: clustered but not tandemly repeated.

1. Replication-dependent histone gene expression is related to Cajal body (CB) association but does not require sustained CB contact.

Review 2. Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.

Review 3. Growth regulation of human variant histone genes and acetylation of the encoded proteins.

4. A comprehensive compilation and alignment of histones and histone genes.

5. Dynamic regulation of histone modifications in Xenopus oocytes through histone exchange.

6. The mRNA coding for the nucleosomal protein H2A of Leishmania is polyadenylated and has stem-loops at the 3' end.

7. Nuclear export of metazoan replication-dependent histone mRNAs is dependent on RNA length and is mediated by TAP.

8. A genomic clone encoding a novel proliferation-dependent histone H2A.1 mRNA enriched in the poly(A)+ fraction.

9. A unique subspecies of histone H4 mRNA from rat myoblasts contains poly(A).

10. Analysis of histone gene expression in adult tissues of the sea urchins Strongylocentrotus purpuratus and Lytechinus pictus: tissue-specific expression of sperm histone genes.