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Literature DB >> 6457299

Isolation of two clusters of mouse histone genes.

D B Sittman, I M Chiu, C J Pan, R H Cohn, L H Kedes, W F Marzluff.

Abstract

Histone mRNA was partially purified from mouse myeloma cells synchronized in S phase by isoleucine starvation. A cDNA was prepared that contained sequences complementary to all five mouse histone genes. This cDNA was used to screen a library of mouse DNA in lambda phage. The positive clones were screened by hybridization with sea urchin histone gene-specific probes to identify those clones that contained histone genes. Confirmation of this identification was obtained by hybridization with Drosophila histone genes. Two independent clusters of histone genes were isolated. One, MM531, contains regions hybridizing specifically to H3, H4, and H1 and the other, MM221, contains two regions hybridizing specifically to H3 and single regions complementary to H4, H2b, and H2a. They are not part of a simple repeating structure. The nucleotide sequence of the coding region of the H3 gene in MM531 has been determined. This gene could code for a variant H3 protein that has several amino acid substitutions not reported in other H3 proteins.

Entities: Chemical Disease Gene Species

Mesh：

Substances：
Histones

Year: 1981 PMID： 6457299 PMCID： PMC319729 DOI： 10.1073/pnas.78.7.4078

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

35 in total

1. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

Authors: R A Laskey; A D Mills
Journal: Eur J Biochem Date: 1975-08-15

2. Regulation of cell cycle stage-specific transcription of histone genes from chromatin by non-histone chromosomal proteins.

Authors: G Stein; W Park; C Thrall; R Mans; J Stein
Journal: Nature Date: 1975-10-30 Impact factor: 49.962

3. Further evidence of transcriptional and translational control of histone messenger RNA during the HeLa S3 cycle.

Authors: T W Borun; F Gabrielli; K Ajiro; A Zweidler; C Baglioni
Journal: Cell Date: 1975-01 Impact factor: 41.582

4. Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.

Authors: A E Sippel
Journal: Eur J Biochem Date: 1973-08-01

5. In vivo effects of intercalating drugs on the superhelix density of mitochondrial DNA isolated from human and mouse cells in culture.

Authors: C A Smith; J M Jordan; J Vinograd
Journal: J Mol Biol Date: 1971-07-28 Impact factor: 5.469

6. On the translational control of histone synthesis. Quantitation of biologically active histone mRNA from synchronized HeLa cells and its translation in different cell-free systems.

Authors: M Breindl; D Gallwitz
Journal: Eur J Biochem Date: 1974-06-01

Isolation of two clusters of mouse histone genes.

1. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

2. Regulation of cell cycle stage-specific transcription of histone genes from chromatin by non-histone chromosomal proteins.

3. Further evidence of transcriptional and translational control of histone messenger RNA during the HeLa S3 cycle.

4. Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.

5. In vivo effects of intercalating drugs on the superhelix density of mitochondrial DNA isolated from human and mouse cells in culture.

6. On the translational control of histone synthesis. Quantitation of biologically active histone mRNA from synchronized HeLa cells and its translation in different cell-free systems.

7. High resolution acrylamide gel electrophoresis of histones.

8. Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger.

9. The resolution of four lysine-rich histones derived from calf thymus.

10. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

1. Production of transforming growth factor alpha by hamster eosinophils.

2. A comprehensive compilation and alignment of histones and histone genes.

3. Isolation and characterization of a Drosophila hydei histone DNA repeat unit.

4. tRNA derived insertion element in histone gene repeating unit of Drosophila melanogaster.

5. Cloning and characterization of a core histone gene tandem repeat in Urechis caupo.

6. Changes in the levels of three different classes of histone mRNA during murine erythroleukemia cell differentiation.

7. Sequences of four mouse histone H3 genes: implications for evolution of mouse histone genes.

8. Replacement variant histone genes contain intervening sequences.

9. Histone mRNA concentrations are regulated at the level of transcription and mRNA degradation.

10. Organization and expression of cloned histone gene clusters from Xenopus laevis and X. borealis.