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Literature DB >> 2793165

Probe walking: development of novel probes for DNA fingerprinting.

Abstract

The tandem repeat of a 28-base-pair (bp) sequence downstream of the human c-Ha-ras-1 oncogene was studied as a probe for DNA fingerprinting. Multiple hypervariable patterns were observed by Southern hybridization at low stringency. The patterns were specific to individuals, indicating the availability of the 28-bp repeat as a probe for DNA fingerprinting. Moreover, we cloned the tandem repeat of a 33-bp sequence, which cross-hybridized with the 28-bp repeat. This 33-bp repeat detected another set of hypervariable restriction fragments by Southern hybridization at the same stringency. These results suggests that 'probe walking' can be employed to develop novel probes that provide different DNA fingerprints.

Entities: Gene Species

Mesh：

Substances：
DNA Probes

Year: 1989 PMID： 2793165 DOI： 10.1007/bf00285160

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

25 in total

1. Cloning and complete nucleotide sequence of mouse immunoglobulin gamma 1 chain gene.

Authors: T Honjo; M Obata; Y Yamawaki-Katoaka; T Kataoka; T Kawakami; N Takahashi; Y Mano
Journal: Cell Date: 1979-10 Impact factor: 41.582

2. Variable number of tandem repeat (VNTR) markers for human gene mapping.

Authors: Y Nakamura; M Leppert; P O'Connell; R Wolff; T Holm; M Culver; C Martin; E Fujimoto; M Hoff; E Kumlin
Journal: Science Date: 1987-03-27 Impact factor: 47.728

3. Unique allelic restriction fragments of the human Ha-ras locus in leukocyte and tumour DNAs of cancer patients.

Authors: T G Krontiris; N A DiMartino; M Colb; D R Parkinson
Journal: Nature Date: 1985 Jan 31-Feb 6 Impact factor: 49.962

4. Hypervariable 'minisatellite' regions in human DNA.

Authors: A J Jeffreys; V Wilson; S L Thein
Journal: Nature Date: 1985 Mar 7-13 Impact factor: 49.962

5. A hypervariable repeated sequence on human chromosome 1p36.

Authors: N Buroker; R Bestwick; G Haight; R E Magenis; M Litt
Journal: Hum Genet Date: 1987-10 Impact factor: 4.132

6. A variable tandem repeat locus mapped to chromosome band 10q26 is amplified and rearranged in leukocyte DNAs of two cancer patients.

Authors: M Colb; T Yang-Feng; U Francke; B Mermer; D R Parkinson; T G Krontiris
Journal: Nucleic Acids Res Date: 1986-10-24 Impact factor: 16.971

Probe walking: development of novel probes for DNA fingerprinting.

1. Cloning and complete nucleotide sequence of mouse immunoglobulin gamma 1 chain gene.

2. Variable number of tandem repeat (VNTR) markers for human gene mapping.

3. Unique allelic restriction fragments of the human Ha-ras locus in leukocyte and tumour DNAs of cancer patients.

4. Hypervariable 'minisatellite' regions in human DNA.

5. A hypervariable repeated sequence on human chromosome 1p36.

6. A variable tandem repeat locus mapped to chromosome band 10q26 is amplified and rearranged in leukocyte DNAs of two cancer patients.

7. Positive identification of an immigration test-case using human DNA fingerprints.

8. DNA sequencing with chain-terminating inhibitors.

9. Demographic study of a wild house sparrow population by DNA fingerprinting.

10. Two highly polymorphic minisatellites from the pseudoautosomal region of the human sex chromosomes.

1. Non-radioactive in situ hybridization pattern of the M13 minisatellite sequences on human metaphase chromosomes.