Literature DB >> 2791035

Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase.

E I Campbell1, S E Unkles, J A Macro, C van den Hondel, R Contreras, J R Kinghorn.   

Abstract

Aspergillus niger transformation frequencies of up to 1,176 transformants per micrograms DNA were achieved using the plasmid vector pSTA10 containing the A. niger nitrate reductase structural gene. Analysis of genomic endonuclease cleaved DNA from nitrate utilising transformants by DNA hybridisation, showed that most integration events are as a result of homologous recombination. The niaD transformation system was used successfully for the introduction of the unselected Escherichia coli fusion genes lacZ, encoding beta-galactosidase, and uidA, for beta-glucuronidase, as well as the Neurospora crassa tub-2 gene, for beta-tubulin. pSTA10 was also capable of transforming niaD mutants of other filamentous fungi such as A.nidulans, A. oryzae and Penicillium chrysogenum.

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Year:  1989        PMID： 2791035     DOI： 10.1007/bf00411084

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  13 in total

1.  Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli.

Authors:  P J Punt; R P Oliver; M A Dingemanse; P H Pouwels; C A van den Hondel
Journal:  Gene       Date:  1987       Impact factor: 3.688

2.  The oliC3 gene of Aspergillus niger: isolation, sequence and use as a selectable marker for transformation.

Authors:  M Ward; L J Wilson; C L Carmona; G Turner
Journal:  Curr Genet       Date:  1988-07       Impact factor: 3.886

3.  Expression of an Escherichia coli beta-galactosidase fusion gene in Aspergillus nidulans.

Authors:  R F van Gorcom; P H Pouwels; T Goosen; J Visser; H W van den Broek; J E Hamer; W E Timberlake; C A van den Hondel
Journal:  Gene       Date:  1985       Impact factor: 3.688

4.  Expression of the Escherichia coli beta-glucuronidase gene in industrial and phytopathogenic filamentous fungi.

Authors:  I N Roberts; R P Oliver; P J Punt; C A van den Hondel
Journal:  Curr Genet       Date:  1989-03       Impact factor: 3.886

5.  Transformation of Aspergillus nidulans by using a trpC plasmid.

Authors:  M M Yelton; J E Hamer; W E Timberlake
Journal:  Proc Natl Acad Sci U S A       Date:  1984-03       Impact factor: 11.205

6.  Development of a homologous transformation system for Aspergillus niger based on the pyrG gene.

Authors:  W van Hartingsveldt; I E Mattern; C M van Zeijl; P H Pouwels; C A van den Hondel
Journal:  Mol Gen Genet       Date:  1987-01

7.  Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans.

Authors:  F P Buxton; D I Gwynne; R W Davies
Journal:  Gene       Date:  1985       Impact factor: 3.688

8.  Transformation of Aspergillus niger with the homologous nitrate reductase gene.

Authors:  S E Unkles; E I Campbell; D Carrez; C Grieve; R Contreras; W Fiers; C A Van den Hondel; J R Kinghorn
Journal:  Gene       Date:  1989-05-15       Impact factor: 3.688

9.  Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker.

Authors:  M J Orbach; E B Porro; C Yanofsky
Journal:  Mol Cell Biol       Date:  1986-07       Impact factor: 4.272

10.  Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans.

Authors:  J M Kelly; M J Hynes
Journal:  EMBO J       Date:  1985-02       Impact factor: 11.598
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  8 in total

1.  Ku80 gene is related to non-homologous end-joining and genome stability in Aspergillus niger.

Authors:  Jinxiang Zhang; Zhihui Mao; Wei Xue; Ying Li; Guomin Tang; Aoquan Wang; Yingjiu Zhang; Huaming Wang
Journal:  Curr Microbiol       Date:  2011-01-12       Impact factor: 2.188

2.  Penicillopepsin-JT2, a recombinant enzyme from Penicillium janthinellum and the contribution of a hydrogen bond in subsite S3 to k(cat).

Authors:  Q N Cao; M Stubbs; K Q Ngo; M Ward; A Cunningham; E F Pai; G C Tu; T Hofmann
Journal:  Protein Sci       Date:  2000-05       Impact factor: 6.725

3.  Genetic analysis of Aspergillus niger: isolation of chlorate resistance mutants, their use in mitotic mapping and evidence for an eighth linkage group.

Authors:  A J Debets; K Swart; C J Bos
Journal:  Mol Gen Genet       Date:  1990-05

4.  Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase.

Authors:  R Sànchez-Fernàndez; S E Unkles; E I Campbell; J A Macro; E Cerdà-Olmedo; J R Kinghorn
Journal:  Mol Gen Genet       Date:  1991-02

5.  Use of Aspergillus overproducing mutants, cured for integrated plasmid, to overproduce heterologous proteins.

Authors:  M Ward; L J Wilson; K H Kodama
Journal:  Appl Microbiol Biotechnol       Date:  1993-08       Impact factor: 4.813

6.  Transformation frequencies are enhanced and vector DNA is targeted during retransformation of Leptosphaeria maculans, a fungal plant pathogen.

Authors:  M L Farman; R P Oliver
Journal:  Mol Gen Genet       Date:  1992-01

7.  Characterization of humanized antibodies secreted by Aspergillus niger.

Authors:  Michael Ward; Cherry Lin; Doreen C Victoria; Bryan P Fox; Judith A Fox; David L Wong; Hendrik J Meerman; Jeff P Pucci; Robin B Fong; Meng H Heng; Naoya Tsurushita; Christine Gieswein; Minha Park; Huaming Wang
Journal:  Appl Environ Microbiol       Date:  2004-05       Impact factor: 4.792

Review 8.  Genetic Manipulation in Mucorales and New Developments to Study Mucormycosis.

Authors:  Carlos Lax; José Tomás Cánovas-Márquez; Ghizlane Tahiri; Eusebio Navarro; Victoriano Garre; Francisco Esteban Nicolás
Journal:  Int J Mol Sci       Date:  2022-03-22       Impact factor: 5.923
  8 in total

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