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Literature DB >> 2504501

Expression of the Escherichia coli beta-glucuronidase gene in industrial and phytopathogenic filamentous fungi.

I N Roberts¹, R P Oliver, P J Punt, C A van den Hondel.

Abstract

A chimaeric beta-glucuronidase (GUS) gene has been created by ligating the Aspergillus nidulans glyceraldehyde 3-phosphate dehydrogenase promoter to the coding sequence of the E. coli uidA gene. Co-transformation of this vector into A. nidulans, A. niger and the tomato pathogen Fulvia fulva (syn. Cladosporium fulvum (Cooke] resulted in the expression of beta-glucuronidase. GUS activity was detected by growth on agar media containing X-gluc and by enzyme assays of mycelial extracts. Expression of the gene in F. fulva transformants was also easily detectable during growth in plants and did not affect pathogenicity. These results form the basis for a versatile and sensitive reporter gene system for industrial and phytopathogenic filamentous fungi.

Entities: Chemical Species

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Year: 1989 PMID： 2504501 DOI： 10.1007/bf00435503

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

14 in total

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Authors: R F van Gorcom; C A van den Hondel
Journal: Nucleic Acids Res Date: 1988-09-26 Impact factor: 16.971

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Journal: Mol Gen Genet Date: 1987-12

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Authors: R F van Gorcom; P J Punt; P H Pouwels; C A van den Hondel
Journal: Gene Date: 1986 Impact factor: 3.688

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Authors: K Wernars; T Goosen; B M Wennekes; K Swart; C A van den Hondel; H W van den Broek
Journal: Mol Gen Genet Date: 1987-08

5. beta-Glucuronidase from Escherichia coli as a gene-fusion marker.

Authors: R A Jefferson; S M Burgess; D Hirsh
Journal: Proc Natl Acad Sci U S A Date: 1986-11 Impact factor: 11.205

6. Development of a homologous transformation system for Aspergillus niger based on the pyrG gene.

Authors: W van Hartingsveldt; I E Mattern; C M van Zeijl; P H Pouwels; C A van den Hondel
Journal: Mol Gen Genet Date: 1987-01

7. Expression of chimeric genes in Caenorhabditis elegans.

Authors: R A Jefferson; M Klass; N Wolf; D Hirsh
Journal: J Mol Biol Date: 1987-01-05 Impact factor: 5.469

8. The Rhizobium leguminosarum nodulation gene nodF encodes a polypeptide similar to acyl-carrier protein and is regulated by nodD plus a factor in pea root exudate.

Authors: C A Shearman; L Rossen; A W Johnston; J A Downie
Journal: EMBO J Date: 1986-04 Impact factor: 11.598

9. Identification of plant-induced genes of the bacterial pathogen Xanthomonas campestris pathovar campestris using a promoter-probe plasmid.

Authors: A E Osbourn; C E Barber; M J Daniels
Journal: EMBO J Date: 1987-01 Impact factor: 11.598

10. GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.

Authors: R A Jefferson; T A Kavanagh; M W Bevan
Journal: EMBO J Date: 1987-12-20 Impact factor: 11.598

42 in total

1. Requirement for either a host- or pectin-induced pectate lyase for infection of Pisum sativum by Nectria hematococca.

Authors: L M Rogers; Y K Kim; W Guo; L González-Candelas; D Li; P E Kolattukudy
Journal: Proc Natl Acad Sci U S A Date: 2000-08-15 Impact factor: 11.205

Review 2. Biolistic transformation of arbuscular mycorrhizal fungi. Progress and perspectives.

Authors: L A Harrier; S Millam
Journal: Mol Biotechnol Date: 2001-05 Impact factor: 2.695

3. Cotransformation of Trichoderma harzianum with beta-glucuronidase and green fluorescent protein genes provides a useful tool for monitoring fungal growth and activity in natural soils.

Authors: Y S Bae; G R Knudsen
Journal: Appl Environ Microbiol Date: 2000-02 Impact factor: 4.792

4. Endophytic colonization of Vitis vinifera L. by plant growth-promoting bacterium Burkholderia sp. strain PsJN.

Authors: Stéphane Compant; Birgit Reiter; Angela Sessitsch; Jerzy Nowak; Christophe Clément; Essaïd Ait Barka
Journal: Appl Environ Microbiol Date: 2005-04 Impact factor: 4.792

5. Cloning and expression of a chitinase gene from the hyperparasitic fungus Aphanocladium album.

Authors: P L Blaiseau; C Kunz; R Grison; Y Bertheau; Y Brygoo
Journal: Curr Genet Date: 1992-01 Impact factor: 3.886

6. Design and production in Aspergillus niger of a chimeric protein associating a fungal feruloyl esterase and a clostridial dockerin domain.

Authors: Anthony Levasseur; Sandrine Pagès; Henri-Pierre Fierobe; David Navarro; Peter Punt; Jean-Pierre Belaïch; Marcel Asther; Eric Record
Journal: Appl Environ Microbiol Date: 2004-12 Impact factor: 4.792

7. Foreign DNA sequences are received by a wild-type strain of Aspergillus niger after co-culture with transgenic higher plants.

Authors: T Hoffmann; C Golz; O Schieder
Journal: Curr Genet Date: 1994-12 Impact factor: 3.886

8. Nitrogen limitation induces expression of the avirulence gene avr9 in the tomato pathogen Cladosporium fulvum.

Authors: G F Van den Ackerveken; R M Dunn; A J Cozijnsen; J P Vossen; H W Van den Broek; P J De Wit
Journal: Mol Gen Genet Date: 1994-05-10

9. The AVR9 race-specific elicitor of Cladosporium fulvum is processed by endogenous and plant proteases.

Authors: G F Van den Ackerveken; P Vossen; P J De Wit
Journal: Plant Physiol Date: 1993-09 Impact factor: 8.340

10. The GUS gene fusion system (Escherichia coli beta-D-glucuronidase gene), a useful tool in studies of root colonization by Fusarium oxysporum.

Authors: Y Couteaudier; M J Daboussi; A Eparvier; T Langin; J Orcival
Journal: Appl Environ Microbiol Date: 1993-06 Impact factor: 4.792