Literature DB >> 2901295

The oliC3 gene of Aspergillus niger: isolation, sequence and use as a selectable marker for transformation.

M Ward1, L J Wilson, C L Carmona, G Turner.   

Abstract

The oliC3 gene of Aspergillus niger has been isolated and sequenced. This gene encodes an oligomycin-resistant variant of the mitochondrial ATP synthase subunit 9. In transformation experiments the gene can serve as a semi-dominant selectable marker for A. niger. It was possible to recognize transformants in which oliC3 had integrated at the homologous oliC locus, as opposed to elsewhere in the genome, by observation of phenotypes on medium containing oligomycin. DNA sequencing has allowed comparison of the deduced amino acid sequence with subunit 9 proteins from other species and comparison of 5' untranslated sequences with those from other fungi.

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Year:  1988        PMID: 2901295     DOI: 10.1007/bf00405851

Source DB:  PubMed          Journal:  Curr Genet        ISSN: 0172-8083            Impact factor:   3.886


  22 in total

1.  Identification of two products of mitochondrial protein synthesis associated with mitochondrial adenosine triphosphatase from Neurospora crassa.

Authors:  G Jackl; W Sebald
Journal:  Eur J Biochem       Date:  1975-05

2.  Nuclear-extranuclear interactions affecting oligomycin resistance in Aspergillus nidulans.

Authors:  R T Rowlands; G Turner
Journal:  Mol Gen Genet       Date:  1977-09-09

3.  Lambda replacement vectors carrying polylinker sequences.

Authors:  A M Frischauf; H Lehrach; A Poustka; N Murray
Journal:  J Mol Biol       Date:  1983-11-15       Impact factor: 5.469

4.  Transformation of Aspergillus nidulans with a cloned, oligomycin-resistant ATP synthase subunit 9 gene.

Authors:  M Ward; B Wilkinson; G Turner
Journal:  Mol Gen Genet       Date:  1986-02

5.  A system for shotgun DNA sequencing.

Authors:  J Messing; R Crea; P H Seeburg
Journal:  Nucleic Acids Res       Date:  1981-01-24       Impact factor: 16.971

6.  Development of a homologous transformation system for Aspergillus niger based on the pyrG gene.

Authors:  W van Hartingsveldt; I E Mattern; C M van Zeijl; P H Pouwels; C A van den Hondel
Journal:  Mol Gen Genet       Date:  1987-01

7.  Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans.

Authors:  F P Buxton; D I Gwynne; R W Davies
Journal:  Gene       Date:  1985       Impact factor: 3.688

8.  Processing peptidase of Neurospora mitochondria. Two-step cleavage of imported ATPase subunit 9.

Authors:  B Schmidt; E Wachter; W Sebald; W Neupert
Journal:  Eur J Biochem       Date:  1984-11-02

9.  The imported preprotein of the proteolipid subunit of the mitochondrial ATP synthase from Neurospora crassa. Molecular cloning and sequencing of the mRNA.

Authors:  A Viebrock; A Perz; W Sebald
Journal:  EMBO J       Date:  1982       Impact factor: 11.598

10.  Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans.

Authors:  J M Kelly; M J Hynes
Journal:  EMBO J       Date:  1985-02       Impact factor: 11.598

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  11 in total

1.  Regulation of the glaA gene of Aspergillus niger.

Authors:  T Fowler; R M Berka; M Ward
Journal:  Curr Genet       Date:  1990-12       Impact factor: 3.886

2.  Aspergillus nidulans nuclear proteins bind to a CCAAT element and the adjacent upstream sequence in the promoter region of the starch-inducible Taka-amylase A gene.

Authors:  O Nagata; T Takashima; M Tanaka; N Tsukagoshi
Journal:  Mol Gen Genet       Date:  1993-02

3.  Purification and characterisation of an Aspergillus niger invertase and its DNA sequence.

Authors:  L M Boddy; T Bergès; C Barreau; M H Vainstein; M J Dobson; D J Ballance; J F Peberdy
Journal:  Curr Genet       Date:  1993 Jul-Aug       Impact factor: 3.886

4.  Transformation frequencies are enhanced and vector DNA is targeted during retransformation of Leptosphaeria maculans, a fungal plant pathogen.

Authors:  M L Farman; R P Oliver
Journal:  Mol Gen Genet       Date:  1992-01

5.  Screening of basidiomycetes for lignin peroxidase genes using a DNA probe.

Authors:  Y Kimura; Y Asada; M Kuwahara
Journal:  Appl Microbiol Biotechnol       Date:  1990-01       Impact factor: 4.813

6.  Improved transformation frequency and heterologous promoter recognition in Aspergillus niger.

Authors:  G Mohr; K Esser
Journal:  Appl Microbiol Biotechnol       Date:  1990-10       Impact factor: 4.813

7.  Identification of a minimal cre1 promoter sequence promoting glucose-dependent gene expression in the beta-lactam producer Acremonium chrysogenum.

Authors:  Danielle Janus; Peter Hortschansky; Ulrich Kück
Journal:  Curr Genet       Date:  2007-11-27       Impact factor: 3.886

8.  Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase.

Authors:  E I Campbell; S E Unkles; J A Macro; C van den Hondel; R Contreras; J R Kinghorn
Journal:  Curr Genet       Date:  1989-07       Impact factor: 3.886

Review 9.  Transformation in fungi.

Authors:  J R Fincham
Journal:  Microbiol Rev       Date:  1989-03

10.  Sequence of the nuclear ATP synthase subunit 9 gene of Podospora anserina: lack of similarity to the mitochondrial genome.

Authors:  R Ridder; K P Künkele; H D Osiewacz
Journal:  Curr Genet       Date:  1991-09       Impact factor: 3.886

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