Literature DB >> 2005864

Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase.

R Sànchez-Fernàndez1, S E Unkles, E I Campbell, J A Macro, E Cerdà-Olmedo, J R Kinghorn.   

Abstract

A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per microgram DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.

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Year:  1991        PMID： 2005864     DOI： 10.1007/bf00269853

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  7 in total

1.  Transformation of seven species of filamentous fungi using the nitrate reductase gene of Aspergillus nidulans.

Authors:  M J Daboussi; A Djeballi; C Gerlinger; P L Blaiseau; I Bouvier; M Cassan; M H Lebrun; D Parisot; Y Brygoo
Journal:  Curr Genet       Date:  1989-06       Impact factor: 3.886

Review 2.  Genetic studies of nitrate assimilation in Aspergillus nidulans.

Authors:  D J Cove
Journal:  Biol Rev Camb Philos Soc       Date:  1979-08

3.  Gibberella fujikuroi mutants obtained with UV radiation and N-methyl-N'-nitro-N-nitrosoguanidine.

Authors:  J Avalos; J Casadesús; E Cerdá-Olmedo
Journal:  Appl Environ Microbiol       Date:  1985-01       Impact factor: 4.792

4.  Homologous transformation of Cephalosporium acremonium with the nitrate reductase-encoding gene (niaD).

Authors:  M P Whitehead; S J Gurr; C Grieve; S E Unkles; D Spence; M Ramsden; J R Kinghorn
Journal:  Gene       Date:  1990-06-15       Impact factor: 3.688

5.  Nitrate reduction mutants of fusarium moniliforme (gibberella fujikuroi).

Authors:  C Klittich; J F Leslie
Journal:  Genetics       Date:  1988-03       Impact factor: 4.562

6.  Cloning of the nitrate reductase gene (niaD) of Aspergillus nidulans and its use for transformation of Fusarium oxysporum.

Authors:  L Malardier; M J Daboussi; J Julien; F Roussel; C Scazzocchio; Y Brygoo
Journal:  Gene       Date:  1989-05-15       Impact factor: 3.688

7.  Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase.

Authors:  E I Campbell; S E Unkles; J A Macro; C van den Hondel; R Contreras; J R Kinghorn
Journal:  Curr Genet       Date:  1989-07       Impact factor: 3.886
  7 in total
  9 in total

1.  Secondary metabolite production in filamentous fungi displayed.

Authors:  V C Appleyard; S E Unkles; M Legg; J R Kinghorn
Journal:  Mol Gen Genet       Date:  1995-05-10

Review 2.  Recent advances in metabolic regulation and bioengineering of gibberellic acid biosynthesis in Fusarium fujikuroi.

Authors:  Hao-Nan Wang; Xia Ke; Jun-Ping Zhou; Zhi-Qiang Liu; Yu-Guo Zheng
Journal:  World J Microbiol Biotechnol       Date:  2022-06-11       Impact factor: 3.312

3.  A homologous and self-replicating system for efficient transformation of Fusarium oxysporum.

Authors:  M D Garcia-Pedrajas; M I Roncero
Journal:  Curr Genet       Date:  1996-01       Impact factor: 3.886

4.  Nitrate reductase of the ascomycetous fungus, Leptosphaeria maculans: gene sequence and chromosomal location.

Authors:  R S Williams; M A Davis; B J Howlett
Journal:  Mol Gen Genet       Date:  1994-07-08

5.  Transformation of Gibberella fujikuroi: effect of the Aspergillus nidulans AMA1 sequence on frequency and integration.

Authors:  B Brückner; S E Unkles; K Weltring; J R Kinghorn
Journal:  Curr Genet       Date:  1992-10       Impact factor: 3.886

6.  The regulatory gene nit-2 of Neurospora crassa complements a nnu mutant of Gibberella zeae (Fusarium graminearum).

Authors:  M B Dickman; J F Leslie
Journal:  Mol Gen Genet       Date:  1992-11

Review 7.  Genetics and gibberellin production in Gibberella fujikuroi.

Authors:  E Cerdá-Olmedo; R Fernández-Martín; J Avalos
Journal:  Antonie Van Leeuwenhoek       Date:  1994       Impact factor: 2.271

8.  Heritability of fumonisin B1 production in Gibberella fujikuroi mating population A.

Authors:  A E Desjardins; R D Plattner; D D Shackelford; J F Leslie; P E Nelson
Journal:  Appl Environ Microbiol       Date:  1992-09       Impact factor: 4.792

Review 9.  The Gibberellin Producer Fusarium fujikuroi: Methods and Technologies in the Current Toolkit.

Authors:  Yu-Ke Cen; Jian-Guang Lin; You-Liang Wang; Jun-You Wang; Zhi-Qiang Liu; Yu-Guo Zheng
Journal:  Front Bioeng Biotechnol       Date:  2020-03-27
  9 in total

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