Literature DB >> 27499108

Implementation of high slurry concentration and sonication to pack high-efficiency, meter-long capillary ultrahigh pressure liquid chromatography columns.

Justin M Godinho1, Arved E Reising2, Ulrich Tallarek3, James W Jorgenson4.   

Abstract

Slurry packing capillary columns for ultrahigh pressure liquid chromatography is complicated by many interdependent experimental variables. Previous results have suggested that combination of high slurry concentration and sonication during packing would create homogeneous bed microstructures and yield highly efficient capillary columns. Herein, the effect of sonication while packing very high slurry concentrations is presented. A series of six, 1m×75μm internal diameter columns were packed with 200mg/mL slurries of 2.02μm bridged-ethyl hybrid silica particles. Three of the columns underwent sonication during packing and yielded highly efficient separations with reduced plate heights as low as 1.05.
Copyright © 2016 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  High efficiency; High slurry concentrations; Packed capillary columns; Sonication; UHPLC

Mesh：

Substances：

Year:  2016        PMID： 27499108      PMCID： PMC5501974          DOI： 10.1016/j.chroma.2016.08.002

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  25 in total

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Authors:  R A Shalliker; B S Broyles; G Guiochon
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2.  Hydrodynamic aspects of slurry packing processes in microcolumn liquid chromatography.

Authors:  J P Vissers; M A Hoeben; J Laven; H A Claessens; C A Cramers
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3.  Comparison of different packing methods for capillary electrochromatography columns.

Authors:  S Roulin; R Dmoch; R Carney; K D Bartle; P Myers; M R Euerby; C Johnson
Journal:  J Chromatogr A       Date:  2000-07-28       Impact factor: 4.759

4.  From random sphere packings to regular pillar arrays: effect of the macroscopic confinement on hydrodynamic dispersion.

Authors:  Anton Daneyko; Siarhei Khirevich; Alexandra Höltzel; Andreas Seidel-Morgenstern; Ulrich Tallarek
Journal:  J Chromatogr A       Date:  2011-09-21       Impact factor: 4.759

5.  Larger voids in mechanically stable, loose packings of 1.3μm frictional, cohesive particles: Their reconstruction, statistical analysis, and impact on separation efficiency.

Authors:  Arved E Reising; Justin M Godinho; Kristof Hormann; James W Jorgenson; Ulrich Tallarek
Journal:  J Chromatogr A       Date:  2016-01-30       Impact factor: 4.759

6.  How does column packing microstructure affect column efficiency in liquid chromatography?

Authors:  Mark R Schure; Robert S Maier
Journal:  J Chromatogr A       Date:  2006-06-27       Impact factor: 4.759

7.  Influence of viscous friction heating on the efficiency of columns operated under very high pressures.

Authors:  Fabrice Gritti; Michel Martin; Georges Guiochon
Journal:  Anal Chem       Date:  2009-05-01       Impact factor: 6.986

8.  Preparation and evaluation of slurry-packed liquid chromatography microcolumns with inner diameters from 12 to 33 microns.

Authors:  S Hsieh; J W Jorgenson
Journal:  Anal Chem       Date:  1996-04-01       Impact factor: 6.986

Review 9.  Consolidation of particle beds and packing of chromatographic columns.

Authors:  G Guiochon; T Farkas; H Guan-Sajonz; J H Koh; M Sarker; B J Stanley; T Yun
Journal:  J Chromatogr A       Date:  1997-02-21       Impact factor: 4.759

10.  In-depth characterization of slurry packed capillary columns with 1.0-microm nonporous particles using reversed-phase isocratic ultrahigh-pressure liquid chromatography.

Authors:  Kamlesh D Patel; Anton D Jerkovich; Jason C Link; James W Jorgenson
Journal:  Anal Chem       Date:  2004-10-01       Impact factor: 6.986
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4.  Ultrahigh-Performance capillary liquid chromatography-mass spectrometry at 35 kpsi for separation of lipids.

Authors:  Matthew J Sorensen; Kelsey E Miller; James W Jorgenson; Robert T Kennedy
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5.  In-column bonded phase polymerization for improved packing uniformity.

Authors:  Alexis G Huckabee; Charu Yerneni; Rachel E Jacobson; Edwin J Alzate; Tse-Hong Chen; Mary J Wirth
Journal:  J Sep Sci       Date:  2017-05-02       Impact factor: 3.645

6.  Bed morphological features associated with an optimal slurry concentration for reproducible preparation of efficient capillary ultrahigh pressure liquid chromatography columns.

Authors:  Arved E Reising; Justin M Godinho; James W Jorgenson; Ulrich Tallarek
Journal:  J Chromatogr A       Date:  2017-05-04       Impact factor: 4.759

Review 7.  Microfluidics for Peptidomics, Proteomics, and Cell Analysis.

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Journal:  Nanomaterials (Basel)       Date:  2021-04-26       Impact factor: 5.076

8.  Comparison of microcapillary column length and inner diameter investigated with gradient analysis of lipids by ultrahigh-pressure liquid chromatography-mass spectrometry.

Authors:  Kelsey E Miller; James W Jorgenson
Journal:  J Sep Sci       Date:  2020-10-07       Impact factor: 3.645
  8 in total

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