Preparation and evaluation of slurry-packed liquid chromatography microcolumns with inner diameters from 12 to 33 microns.

Fused silica capillary liquid chromatography columns with inner diameters between 12 and 33 microns were slurry packed with 5 microns octadecylsilane-modified silica particles. Column efficiencies and van Deemter coefficients were compared. A linear decrease of the A term as column diameter was decreased was the most significant contributor to a lower overall plate height at the optimum velocity.