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Literature DB >> 2748598

Shuttle vectors for the archaebacterium Halobacterium volcanii.

Abstract

Progress in archaebacterial molecular biology requires tools for genetic analysis. We describe vectors that can be selected and maintained in either Halobacterium volcanii or Escherichia coli. A genetic determinant for resistance to the 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor mevinolin was isolated by "shotgun cloning" into a derivative of the endogenous H. volcanii plasmid pHV2, to form pWL2, which transforms sensitive H. volcanii to mevinolin resistance at high frequency. The resistance determinant, portions of pHV2, and an ampicillin- and tetracycline-resistance-conferring pBR322 derivative, pAT153, were ligated together to form the shuttle vectors pWL101 and pWL102. We describe conditions for the use of these vectors and provide preliminary definition of regions essential for drug resistance and for plasmid replication and maintenance.

Entities: Chemical Species

Mesh：

Substances：
Lovastatin

Year: 1989 PMID： 2748598 PMCID： PMC297646 DOI： 10.1073/pnas.86.14.5478

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

18 in total

1. Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium.

Authors: R L Charlebois; W L Lam; S W Cline; W F Doolittle
Journal: Proc Natl Acad Sci U S A Date: 1987-12 Impact factor: 11.205

Review 2. Bacterial evolution.

Authors: C R Woese
Journal: Microbiol Rev Date: 1987-06

3. An archaebacterial promoter element for stable RNA genes with homology to the TATA box of higher eukaryotes.

Authors: M Thomm; G Wich
Journal: Nucleic Acids Res Date: 1988-01-11 Impact factor: 16.971

4. Phylogenetic structure of the prokaryotic domain: the primary kingdoms.

Authors: C R Woese; G E Fox
Journal: Proc Natl Acad Sci U S A Date: 1977-11 Impact factor: 11.205

5. Genetic transformation system in the archaebacterium Methanobacterium thermoautotrophicum Marburg.

Authors: V E Worrell; D P Nagle; D McCarthy; A Eisenbraun
Journal: J Bacteriol Date: 1988-02 Impact factor: 3.490

6. The complete nucleotide sequence of the arcaebacterial plasmid pHSB from Halobacterium, strain SB3.

Authors: V K Kagramanova; N I Derckacheva; A S Mankin
Journal: Nucleic Acids Res Date: 1988-05-11 Impact factor: 16.971

7. RNA polymerase-binding and transcription initiation sites upstream of the methyl reductase operon of Methanococcus vannielii.

Authors: M Thomm; B A Sherf; J N Reeve
Journal: J Bacteriol Date: 1988-04 Impact factor: 3.490

8. ISH51: a large, degenerate family of insertion sequence-like elements in the genome of the archaebacterium, Halobacterium volcanii.

Authors: J D Hofman; L C Schalkwyk; W F Doolittle
Journal: Nucleic Acids Res Date: 1986-09-11 Impact factor: 16.971

9. Characterization of the small endogenous plasmid of Halobacterium strain SB3 and its use in transformation of H. halobium.

Authors: N R Hackett; S DasSarma
Journal: Can J Microbiol Date: 1989-01 Impact factor: 2.419

10. Efficient transfection of the archaebacterium Halobacterium halobium.

Authors: S W Cline; W F Doolittle
Journal: J Bacteriol Date: 1987-03 Impact factor: 3.490

83 in total

1. An improved transposon for the halophilic archaeon Haloarcula hispanica.

Authors: W G Woods; K Ngui; M L Dyall-Smith
Journal: J Bacteriol Date: 1999-11 Impact factor: 3.490

2. Primary structure and functional analysis of the soluble transducer protein HtrXI in the archaeon Halobacterium salinarium.

Authors: A Brooun; W Zhang; M Alam
Journal: J Bacteriol Date: 1997-05 Impact factor: 3.490

3. Development of a gene knockout system for the halophilic archaeon Haloferax volcanii by use of the pyrE gene.

Authors: Gili Bitan-Banin; Ron Ortenberg; Moshe Mevarech
Journal: J Bacteriol Date: 2003-02 Impact factor: 3.490

4. Localizing genes on the map of the genome of Haloferax volcanii, one of the Archaea.

Authors: A Cohen; W L Lam; R L Charlebois; W F Doolittle; L C Schalkwyk
Journal: Proc Natl Acad Sci U S A Date: 1992-03-01 Impact factor: 11.205

5. Genes for tryptophan biosynthesis in the archaebacterium Haloferax volcanii.

Authors: W L Lam; A Cohen; D Tsouluhas; W F Doolittle
Journal: Proc Natl Acad Sci U S A Date: 1990-09 Impact factor: 11.205

6. Protonatable residues at the cytoplasmic end of transmembrane helix-2 in the signal transducer HtrI control photochemistry and function of sensory rhodopsin I.

Authors: K H Jung; J L Spudich
Journal: Proc Natl Acad Sci U S A Date: 1996-06-25 Impact factor: 11.205

7. Molecular characterization of the phaECHm genes, required for biosynthesis of poly(3-hydroxybutyrate) in the extremely halophilic archaeon Haloarcula marismortui.

Authors: Jing Han; Qiuhe Lu; Ligang Zhou; Jian Zhou; Hua Xiang
Journal: Appl Environ Microbiol Date: 2007-08-03 Impact factor: 4.792

8. Membrane assembly of bacterio-opsin mutants expressed in halobacteria and incorporation of the proteins into phospholipid bilayers.

Authors: M Teintze; Z J Xu
Journal: Biophys J Date: 1992-04 Impact factor: 4.033

9. Transformation of Halobacterium halobium: development of vectors and investigation of gas vesicle synthesis.

Authors: U Blaseio; F Pfeifer
Journal: Proc Natl Acad Sci U S A Date: 1990-09 Impact factor: 11.205

10. Precise determination, cross-recognition, and functional analysis of the double-strand origins of the rolling-circle replication plasmids in haloarchaea.

Authors: Ligang Zhou; Meixian Zhou; Chaomin Sun; Jing Han; Qiuhe Lu; Jian Zhou; Hua Xiang
Journal: J Bacteriol Date: 2008-06-20 Impact factor: 3.490